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A kind of tobacco yellow soil-derived bacteria

A technology of yellow and tobacco, applied in the direction of tobacco, tobacco preparation, bacteria, etc., can solve the problem of unsatisfactory aroma enhancement effect, achieve the effect of reducing smoke green miscellaneous gas, reducing irritation, and increasing the richness of smoke aroma

Active Publication Date: 2022-07-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few microorganisms applied to cigarettes, and the flavoring effect is not ideal

Method used

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  • A kind of tobacco yellow soil-derived bacteria
  • A kind of tobacco yellow soil-derived bacteria
  • A kind of tobacco yellow soil-derived bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Isolation, culture and identification of tobacco yellow soil-derived bacteria X7X

[0030] 1.1 Isolation of tobacco yellow soil-derived bacteria X7X

[0031] Soil samples were taken from the tobacco rhizosphere of the tobacco production base in Kunming City, Yunnan Province, sealed in plastic bags, and stored at 4°C until use. Accurately weigh 10g soil sample, add 100mL sterile water, shake at 30°C, 200rpm for 30min; dilute the concentration to 10 with sterile water -3 , 10 -4w / v times, 0.1 mL of each concentration of bacterial liquid was evenly spread on the R2A agar plate, and three parallel experiments were set up for each concentration of bacterial liquid. Pick up single colonies of different shapes, continue to use plate streak method to isolate and purify strains, inoculate on R2A slant medium after numbering, or prepare into glycerol suspension (20%, w / v), and store at -80 °C .

[0032] The isolated microorganisms were inoculated in R2A liquid medium, 30 °...

Embodiment 2

[0049] Preparation of Bio-flavor from Tobacco Yellow Soil-derived Bacteria X7X Fermentation Broth

[0050] 1. Cultivation of tobacco yellow soil-derived bacteria X7X

[0051] (1) Test tube slope culture

[0052] The slant preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: glucose 0.5g, yeast extract 0.5g, peptone 0.5g, acid hydrolyzed casein 0.5g, soluble starch 0.5g, sodium pyruvate 0.3g , 0.3g of dipotassium hydrogen phosphate, 0.05g of magnesium sulfate, 15g of agar, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on an inclined plane, inoculate tobacco yellow soil-derived bacteria X7X, and culture at 30°C for 2 weeks to obtain test-tube strains;

[0053] (2) Seed cultivation

[0054] The seed culture medium is R2A liquid medium, the formula is: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; hydrogen phosphat...

Embodiment 3

[0068] In order to investigate the uniqueness of tobacco yellow soil source bacteria X7X for preparing fermentation broth biological flavor, embodiment 3 makes the following adjustments:

[0069] The difference between Example 3 and Example 2 is that the relative strain Flavisolibacterswuensis.SR2-4-2 (purchased from the Japanese Collection of Microorganisms (JCM)), which is a relative of tobacco yellow soil-derived bacteria X7X, is used instead of tobacco yellow soil-derived bacteria X7X to prepare fermentation broth. Bio-flavor, other operation steps are the same as step 1 and step 2(1) in Example 2, and a light yellow liquid is obtained, which is the fermentation broth to be prepared. It was found by smelling that the fermentation broth had no fragrance. The fermented liquid was evenly sprayed on the cut tobacco with a micro sprayer in an amount of 0.3‰, rolled into cigarettes, put into a constant temperature and humidity box, and equilibrated for 24h under the conditions o...

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Abstract

The invention provides a tobacco yellow soil-derived bacteria, a separation method and application thereof, and the microbiological classification of the bacteria is named tobacco yellow soil-derived bacteria Flavisolibacter sp.X7X, which has been preserved in China Microbial Culture Collection on September 10, 2018 Deposited by the General Microbiology Center of the Management Committee, the deposit number is CGMCC 16451. The tobacco yellow soil-derived bacteria X7X isolated from the soil can be used for fermentation to prepare biological flavors. The biological flavor, when added to the cigarette cut tobacco, can make the cigarette have a special aroma, and when the cigarette is smoked, it can enrich the aroma of the cigarette, reduce the green and miscellaneous gas in the smoke, increase the fineness and reduce the irritation, and is suitable for cigarette flavoring.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a tobacco yellow soil source bacteria and a separation method and application thereof. Background technique [0002] Flavoring is a key link in the cigarette process, a decisive factor for improving product style and one of the effective means to improve the quality of cigarette aroma. At present, tobacco flavors are divided into three categories according to their sources, namely natural plant essential oils and extracts from non-tobacco; natural plant flavors from non-tobacco, such as flowers, fruits, roots, stems and leaves of various plants Extracted essential oil extracts, tinctures, etc.; synthetic fragrances, such as monomer fragrances such as alcohols, aldehydes, and ketones. According to the different production methods of spices, it is mainly divided into two types, namely natural spices extracted directly from tobacco or other plants by various separation mean...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12P1/04A24B3/12C12R1/01
CPCY02A50/30
Inventor 高莉段焰青李源栋者为王坚李文均陈兴
Owner CHINA TOBACCO YUNNAN IND
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