Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting total protein of momordica grosvenori leaf for proteomic analysis

A technology of proteomics and extraction method, which is applied in the field of extraction of total protein from Luo Han Guo leaves, can solve the problem of inability to effectively remove polyphenols and salts, and achieve the effects of less pollution, improved extraction efficiency, and high-efficiency extraction

Inactive Publication Date: 2019-05-10
ZHEJIANG SCI-TECH UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a method for extracting the total protein of Luo Han Guo leaves for proteomic analysis. The method of the present invention can solve the problem that the traditional phenol extraction method cannot effectively remove polyphenols and Due to the problem of salt substances, the efficient extraction of total protein from the leaves of Luo Han Guo has been realized, and the obtained protein has high purity, which can be applied to proteomic analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] S1: Cut the leaves of Luo Han Guo into pieces, add liquid nitrogen and polyvinyl pyrrolidone, and grind; grind the leaves of Luo Han Guo and mix them with supercritical carbon dioxide at a mass ratio of 1:16-20, put them into a supercritical extraction tank, and the extraction pressure is 18-22MPa, the extraction temperature is 30-33°C, take out the powder after extracting the lipid and mix it with water at a mass ratio of 1:80-120, stir for 5-15min, add sodium bicarbonate with a molar concentration of 0.4-0.6mol / L The solution was left to stand at 20-25° C. for 2-4 hours until the pH value was 9.2-9.6, washed with water until the pH value was 6.8-7.1, filtered, and freeze-dried to obtain Luo Han Guo leaf powder.

[0030] Mix the pretreated Luo Han Guo leaf powder with the protein extract, let it stand at -20°C for 10h, centrifuge at 4°C for 30min at a speed of 12000r / min, and remove the lower layer of sediment, wherein the protein extract is added in an amount of Add 2...

Embodiment 2

[0037] S1: Cut the leaves of Luo Han Guo into pieces, add liquid nitrogen and polyvinyl pyrrolidone, and grind; grind the leaves of Luo Han Guo and mix them with supercritical carbon dioxide at a mass ratio of 1:16, put them into a supercritical extraction tank, and the extraction pressure is 18MPa. The extraction temperature is 30°C, take out the extracted lipid powder and mix it with water at a mass ratio of 1:80, stir for 15 minutes, add a sodium bicarbonate solution with a molar concentration of 0.4mol / L until the pH value is 9.2, and let stand at 20°C After 4 hours, wash with water until the pH value is 6.8, filter, and freeze-dry to obtain the powder of Luo Han Guo leaves.

[0038] Mix the pretreated Luo Han Guo leaf powder with the protein extract, let it stand at -20°C for 10h, centrifuge at 4°C for 30min at a speed of 12000r / min, and remove the lower layer of sediment, wherein the protein extract is added in an amount of Add 3ml per 1g of Luo Han Guo leaf powder.

[...

Embodiment 3

[0045] S1: Cut the leaves of Luo Han Guo into pieces, add liquid nitrogen and polyvinyl pyrrolidone, and grind; grind the leaves of Luo Han Guo and mix them with supercritical carbon dioxide at a mass ratio of 1:20, put them into a supercritical extraction tank, and the extraction pressure is 22MPa. The extraction temperature is 33°C, take out the extracted lipid powder and mix it with water at a mass ratio of 1:120, stir for 5 minutes, add a sodium bicarbonate solution with a molar concentration of 0.6mol / L until the pH value is 9.6, and let stand at 25°C For 2 hours, wash with water until the pH value is 7.1, filter, and freeze-dry to obtain the powder of Luo Han Guo leaves.

[0046] Mix the pretreated Luo Han Guo leaf powder with the protein extract, let it stand at -20°C for 10h, centrifuge at 4°C for 30min at a speed of 12000r / min, and remove the lower layer of sediment, wherein the protein extract is added in an amount of Add 4ml per 1g of Luo Han Guo leaf powder.

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biology, and discloses a method for extracting a total protein of a momordica grosvenori leaf for proteomic analysis. The method comprises steps of S1 mixing pretreated momordica grosvenori leaf powder with a protein extract, standing, centrifuging, and taking a lower layer precipitate; S2 freezing and drying the precipitate, pulverizing to obtain precipitatepowder, and storing for prepare use; S3 mixing the precipitate powder with a protein lysis solution, and conducting pyrolysis to obtain the momordica grosvenori leaf lysis solution; S4 centrifuging the momordica grosvenori leaf lysis solution, taking a supernatant, and drying to obtain the total protein of the momordica grosvenori leaf. The method can solve the problem that a traditional phenol extraction method cannot effectively remove polyphenols and salt substances when a plant leaf protein is produced, the high-efficiency extraction of the total protein of the momordica grosvenori leaf isachieved, the protein purity is high, and the method can be applied to the proteomics analysis.

Description

technical field [0001] The present invention relates to the field of biology, in particular to a method for extracting total protein from Luo Han Guo leaves for proteomics analysis Background technique [0002] Luo Han Guo belongs to Cucurbitaceae and is a dioecious plant of the genus Luo Han Guo, which has important medical and health value. Luo Han Guo fruit has high nutritional value and is rich in fructose, glucose, protein and multivitamins. It is cool in nature and sweet in taste. Luo Han Guo is high in sweetness, low in calories, and non-toxic. It can be used as an ideal food additive for obese patients, diabetics, and hypertensive patients. It has been recorded in the previous "Chinese Pharmacopoeia" that it has the effects of clearing away heat and moistening the lungs, smoothing the intestines and laxatives, and is used to treat dry cough due to lung fire. According to modern pharmacological research, its pharmacological active ingredient is mainly Monk Fruit Sap...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/14
CPCY02P20/54
Inventor 朱诚胡铭周杨慧周莲王秉
Owner ZHEJIANG SCI-TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com