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Systemic lupus erythematosus detection reagent containing lncRNA and usage thereof

A systemic technology for lupus erythematosus, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/testing, etc., can solve the problem of less attention to non-coding RNA, the number of diagnostic markers that cannot reach clinical applications, lupus erythematosus Problems such as protracted and repeated disease course

Inactive Publication Date: 2019-05-14
石家庄市第一医院
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Problems solved by technology

The course of systemic lupus erythematosus is protracted and repeated. It is a complex disease with multiple factors, and its etiology and pathogenesis have not yet been clarified.
In the past, it was believed that autoimmune-related protein-coding genes played an important role in the occurrence and development of diseases, while less attention was paid to non-coding RNAs in the genome. The latest research shows that 1ncRNAs may play an important role in the progression of autoimmune diseases Many reports have preliminarily disclosed the correlation between the 1ncRNAs studied and SLE. Among them, GAS5, lnc-DC, linc0597 and linc0949 in the long non-coding RNAs may be involved in the pathogenesis of SLE and are expected to become biomarkers for SLE disease diagnosis , but the existing diagnostic markers related to SLE disease are far from reaching the number of clinical applications

Method used

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  • Systemic lupus erythematosus detection reagent containing lncRNA and usage thereof
  • Systemic lupus erythematosus detection reagent containing lncRNA and usage thereof
  • Systemic lupus erythematosus detection reagent containing lncRNA and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Integrated analysis based on high-throughput transcriptome data

[0031] 1. Data retrieval

[0032] The GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information), and the GEO database is the largest database of gene expression data. Data retrieval is carried out by searching keywords ("lupuserythematosus,systemic"[MeSH Terms]OR systemic lupus erythematosus[AllFields]) AND "Homo sapiens"[porgn]AND"gse"[Filter], and the retrieved results are screened. The following standard datasets will be included in our research:

[0033] ①The selected data set must be the transcriptome data of the whole genome;

[0034] ② These data come from the blood of systemic lupus erythematosus case group and healthy control group;

[0035] ③ This study considers standardized or original data sets.

[0036] After screening, 6 sets of mRNA data sets and 2 sets of lncRNA data sets were obtained (see Table 1).

[0037] ...

Embodiment 2

[0045] Example 2 Fluorescent quantitative PCR verification of differentially expressed ENSG00000249309 and its adjacent gene TLR2

[0046] 1. Sample collection

[0047] For sample collection, 23 cases of peripheral blood from patients with systemic lupus erythematosus diagnosed in our hospital were selected. The cases all met the SLE diagnostic criteria revised by the American Rheumatology Association. 1mg / Kg / d) completed blood sample collection before. The normal control group consisted of 18 healthy subjects. Infection, tumor, hypertension, diabetes and other systemic diseases were excluded. There were no statistically significant differences in gender and age among the groups. Explain the purpose of the study to the research subjects, and with their consent, use EDTA anticoagulant blood collection tubes to extract 5ml of peripheral venous blood from SLE cases and healthy controls respectively, conduct plasma separation and PBMCs extraction as soon as possible, and put th...

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Abstract

The invention discloses a systemic lupus erythematosus detection reagent containing lncRNA and usage thereof. The lncRNA can regulate the expression of genes at various levels such as an epigenetic level, a transcription level, a post-transcriptional level, protein metabolism and the like, and the expression disorder is closely related to the occurrence and development of many human diseases. In order to discover novel molecular markers related to systemic lupus erythematosus, the reagent performs multi-center and multi-sample data integration analysis on existing high-throughput transcriptomesequencing data, and discovers the novel lncRNA and related genes, and the novel lncRNA provided for the systemic lupus erythematosus by the reagent has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the field of disease molecular diagnosis, and in particular relates to markers for auxiliary diagnosis of systemic lupus erythematosus and applications thereof. Background technique [0002] 1ncRNA is a research hotspot in recent years. It is a type of non-coding RNA with a length greater than 200 nucleotides. Its structure is similar to mRNA, but there is no open reading frame. According to the positional relationship between 1ncRNA and coding gene, we divided it into five types: sense 1ncRNA; antisense 1ncRNA; bidirectional 1ncRNA; intergenic 1ncRNA; intragenic 1ncRNA. 1ncRNA molecules have a specific and complex secondary space structure, can interact with proteins, DNA and RNA, and can participate in X chromatin silencing as signal molecules, decoy molecules, guide molecules, backbone molecules and competing endogenous RNAs , genome imprinting, cell differentiation, cell proliferation and other important biological activit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/6883
Inventor 曹海育李海滨
Owner 石家庄市第一医院
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