Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A recognition of lysine decarboxylase 129 xe"r 2 "Relaxation switch type" magnetic resonance molecular probe

A lysine decarboxylase and molecular probe technology, which is applied in the field of 129Xe "r2 relaxation efficiency switch type" magnetic resonance molecular probes to achieve high-sensitivity identification and strong penetrability.

Active Publication Date: 2021-05-25
WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The determination method is simple and the determination result is accurate, but it needs to use the toxic organic solvent toluene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A recognition of lysine decarboxylase <sup>129</sup> xe"r <sub>2</sub> "Relaxation switch type" magnetic resonance molecular probe
  • A recognition of lysine decarboxylase <sup>129</sup> xe"r <sub>2</sub> "Relaxation switch type" magnetic resonance molecular probe
  • A recognition of lysine decarboxylase <sup>129</sup> xe"r <sub>2</sub> "Relaxation switch type" magnetic resonance molecular probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 measures the relaxation efficiency of ammonium acetate buffer

[0036] (1) Ammonium acetate is dissolved in ultrapure water, and the solutions that are configured into ammonium acetate concentration as 0mM, 2mM, 4mM, 6mM, 8mM, and 10mM are respectively recorded as solution 1, solution 2, solution 3, solution 4, solution 5, Solution 6;

[0037] (2) Each 2 mL of solution 1, solution 2, solution 3, solution 4, solution 5 and solution 6 prepared in the present embodiment was obtained, and the 400MHz magnetic resonance spectrometer was used to 129 In Xe spectrum mode, it is used in conjunction with a hyperpolarized device (the gas heating temperature is 135°C, the gas flow rate is 0.12 standard liters / minute, and the gas composition is 2% Xe, 8% N in volume percentage 2 and 90% He, of which Xe is 26.4% of natural abundance 129 Xe), the experimental temperature is 298K, the test solution 129 T of Xe in solution 1, solution 2, solution 3, solution 4, solution 5...

Embodiment 2

[0038] Embodiment 2 tests the relaxation efficiency of lysine solution

[0039] (1) Get ammonium acetate and be dissolved in ultrapure water, be configured into the solution that ammonium acetate concentration is 10mM, be recorded as solution 1;

[0040] (2) Take 5 parts of lysine and dissolve them in solution 1 respectively, sonicate until the lysine is completely dissolved, and configure the concentration of lysine to be 0.2μM, 0.4μM, 0.6μM, 0.8μM, 1.0μM, and use acetic acid and Ammonia liquor adjusts the pH of solution respectively, makes the pH of solution be 6.0, is recorded as solution 2, solution 3, solution 4, solution 5, solution 6 respectively;

[0041] (3) 2 mL of solution 1, solution 2, solution 3, solution 4, solution 5, and solution 6 prepared in this embodiment are each taken in 2 mL with a 400 MHz magnetic resonance spectrometer 129 In Xe spectrum mode, it is used in conjunction with a hyperpolarized device (the gas heating temperature is 135°C, the gas flow r...

Embodiment 3

[0042] Embodiment 3 measures the relaxation efficiency of lysine decarboxylase solution

[0043] (1) Get ammonium acetate and be dissolved in ultrapure water, be configured into the solution that ammonium acetate concentration is 10mM, be recorded as solution 1;

[0044] (2) Take 4 parts of lysine decarboxylase, dissolve them in solution 1 respectively, configure the concentration of lysine decarboxylase as 0.5μg / mL, 1μg / mL, 1.5μg / mL, 2μg / mL, add acetic acid and ammonia water Adjust the pH of the solution respectively, so that the pH of the solution is 6.0, which are respectively recorded as solution 2, solution 3, solution 4, and solution 5;

[0045] (3) Each 2 mL of solution 1, solution 2, solution 3, solution 4 and solution 5 prepared in the present embodiment was obtained, and the 400MHz magnetic resonance spectrometer was used to 129 In Xe spectrum mode, it is used in conjunction with a hyperpolarization device (the gas heating temperature is 135°C, the gas flow rate is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biochemical analysis, in particular to a method for recognizing lysine decarboxylase 129 Xe"r 2 Relaxation efficiency switch type "magnetic resonance molecular probe, which contains the following components: cucurbit [6] urea and lysine. The principle of this molecular probe is as follows: a substrate with weak cucurbit [6] urea binding force (lysine acid) is converted by lysine decarboxylase into a strong cucurbit[6]urea-bound product (cadaverine).Before the enzymatic reaction, due to the cucurbit[6]urea 129 Xe and dissolved state 129 The chemical exchange of Xe, the relaxation efficiency of the molecular probe is larger. After adding lysine decarboxylase, lysine was gradually converted to cadaverine by lysine decarboxylase, and cucurbit[6]urea was occupied by cadaverine. Eventually the relaxation efficiency of the molecular probe becomes almost zero. Recognition of lysine decarboxylase by switch of molecular probe relaxation efficiency.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a method for recognizing lysine decarboxylase 129 Xe"r 2 Relaxation Efficiency Switching" Magnetic Resonance Molecular Probes. Background technique [0002] Lysine decarboxylase can catalyze the decarboxylation of L-lysine to generate cadaverine (also known as "1,5-pentanediamine") and carbon dioxide, and is a key enzyme in the biological cadaverine synthesis pathway. The cadaverine produced by its decarboxylation reacts with oxygen free radicals to help eliminate the damage of free radicals in organisms to the body, thereby protecting microbial cells from oxygen poisoning. (Igarashi K, Kashiwagi K. Modulation of cellular function by polyamines. Int J Biochem Cell Biol. 2010, 42(1):39-51.). Endogenous decarboxylation by lysine decarboxylase and accumulation of high intracellular concentrations of pentamethylenediamine can lead to decreased outer membrane permeabil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/527G01N24/08
Inventor 周欣张斌郭茜旎孙献平刘买利叶朝辉
Owner WUHAN INST OF PHYSICS & MATHEMATICS CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com