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A kind of custard apple seed extract for treating choriocarcinoma and its preparation method and application

A choriocarcinoma and extract technology, which is applied in the field of tumor treatment and biomedicine, can solve the problems of shortened survival period, decreased patient's physical fitness and resistance, etc.

Active Publication Date: 2021-08-03
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemotherapy drugs generally have the problem of excessive toxicity and side effects, which often lead to killing one thousand enemies and self-injury eight hundred, sharply reducing the patient's physical fitness and resistance, and greatly shortening the survival period

Method used

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  • A kind of custard apple seed extract for treating choriocarcinoma and its preparation method and application
  • A kind of custard apple seed extract for treating choriocarcinoma and its preparation method and application
  • A kind of custard apple seed extract for treating choriocarcinoma and its preparation method and application

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preparation example Construction

[0043] The invention provides a kind of preparation method of custard apple seed extract, comprises the steps:

[0044] (1) pulverizing the custard apple seeds to obtain granules;

[0045] (2) mixing the particulate matter with a degreasing solvent, and separating the solid and liquid after degreasing to obtain a filter residue;

[0046](3) mixing the filter residue with the extraction solvent, separating the solid and liquid after extraction to obtain a liquid phase;

[0047] (4) recovering the solvent in the liquid phase to obtain the annuus apple seed extract.

[0048] In the invention, the seeds of the custard apple are first pulverized to obtain granules. In the present invention, the varieties of the custard apple seeds preferably include custard apple, soursop and / or custard apple. The crushed particle size is preferably 2-25 mesh, more preferably 10-18 mesh.

[0049] After the particle is obtained, the present invention mixes the particle with a degreasing solvent,...

Embodiment 1

[0066] Sweet Apple, Ethanol Ultrasonic Extraction (Round-Ethanol Ultrasonic Extraction)

[0067] Take 20 grams of dried custard apple seeds, crush them into 15-mesh particles, add n-hexane to soak and degrease twice (use 200mL of n-hexane each time, shake intermittently, for 5 hours), filter with filter paper, and add 200ml of absolute ethanol to the filter residue , 25°C and 250W ultrasonic extraction twice, each time 30min, filter paper suction filtration, combined two filtrates, 55°C heating and rotary evaporation under reduced pressure to remove the organic solvent, vacuum drying to obtain the ethanol ultrasonic extract.

Embodiment 2

[0069] Annus apple, ultrasonically extracted with ethanol (soil-alcohol ultrasonically extracted)

[0070] Take 20 grams of dried custard apple (commonly known as custard apple) seeds, crush them into 18-mesh particles, add n-hexane to soak and degrease twice (use 200mL of n-hexane each time, shake intermittently, for 5 hours), filter paper suction, and filter residue Add 200ml of absolute ethanol, ultrasonically extract twice at 25°C and 250W for 30min each time, filter with filter paper, combine the two filtrates, heat at 60°C under reduced pressure and rotary evaporate to remove the organic solvent, and dry in vacuo to obtain the ethanol ultrasonic extract.

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Abstract

The invention provides the application of any one or more compounds of formula I, formula II and formula III in the preparation of medicines for treating choriocarcinoma, which belongs to the technical field of tumor medicines. The compounds represented by formula I, formula II and formula III in the present invention are the monomer components in the total lactone extract of Annuus apple. The invention also provides a preparation method of the annuus apple seed extract for treating choriocarcinoma and its product. The IC of the compound represented by formula I, formula II and formula III and the extract (including the effective part) prepared by the preparation method of the present invention on choriocarcinoma JEG‑3 50 The value is less than 10ng / ml. Among them, the IC of the extract on choriocarcinoma JEG‑3 50 The lowest value is 1.269mg / mL; the IC of the effective part on choriocarcinoma JEG‑3 50 The value is 0.4277nM; especially the IC of the compound shown in formula I to choriocarcinoma JEG‑3 50 The value is only 0.1302nM, while the IC50 for most other tumors is often between 1uM‑100uM, with a difference of three orders of magnitude, showing a selective and efficient killing effect on choriocarcinoma JEG‑3.

Description

technical field [0001] The invention belongs to the technical fields of tumor treatment and biomedicine, and in particular relates to an anemone apple seed extract for treating choriocarcinoma, a preparation method and application thereof. Background technique [0002] Malignant tumor is a major disease that seriously endangers human life and health, and has become one of the main causes of death from diseases today. The incidence and mortality of cancer in my country continue to rise, and the situation is grim. At present, the main treatment methods for malignant tumors include surgery, radiotherapy and chemotherapy. Chemotherapy is still the main way to deal with tumors because the recurrence and metastasis of tumors after surgery still requires chemotherapy. Clinicians can optimize the maximum curative effect and minimum toxicity by adjusting the dosage of drugs, helping patients prolong survival time and improve the prognosis of life quality. However, chemotherapy dru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61K36/185A61P35/00C07D307/58
CPCA61K31/365A61K36/185A61P35/00C07D307/58
Inventor 王向涛敖惠李好文王一安郭一飞韩美华
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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