The invention relates to the technical field of
tissue engineering, and particularly discloses a pilose
antler tissue cell culture method which comprises the following steps: S1,
cell in-vitro culture; s2, directional induced differentiation; step S3,
ossification induction; the induction culture medium 1, the induction culture medium 2 and the
ossification induction culture medium are added,
amphotericin B capable of covering the fungal
pollution risk is added into the induction culture medium 1, fetal calf serum contained in the induction culture medium 1 can neutralize
trypsin, recombinant human HGF contained in the induction culture medium 2 can improve the migration ability of cells, and meanwhile, the
ossification induction culture medium can be used for preparing the ossification culture medium. The recombinant human bFGF can synergistically promote proliferation, so that emigrated cells are quickly accumulated, and the situation that the
cell density is too low due to insufficient proliferation after emigration is avoided;
ascorbic acid-2-
phosphate contained in the ossification induction culture medium can avoid oxidation failure of
ascorbic acid, and the
cell emigration efficiency is improved through the synergistic effect of the substances.