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Environment-friendly process for preparing monosodium glutamate

A process, monosodium glutamate technology, applied in the field of environmental protection process for preparing monosodium glutamate, can solve problems such as high acid production, and achieve the effects of improving acid production efficiency, recovering cell enzyme activity, and reducing residual sugar.

Inactive Publication Date: 2019-05-24
卢松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the late stage of acid production by glutamic acid fermentation, a large amount of pyruvic acid or lactic acid will accumulate. At this time, it is necessary to stop the fermentation, remove the bacteria by filtration or centrifugation, and then purify the fermentation broth to obtain the product; however, the discarded bacteria still have With a certain acid production capacity, how to reuse the waste bacteria to facilitate high acid production is a technical problem that we need to solve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] An environmentally friendly process for preparing monosodium glutamate, which comprises the steps of:

[0029] Corynebacterium glutamicum seed liquid (1 × 10 8 cfu / ml) according to the inoculum amount of 8%, inoculated in the fermenter with fermentation medium for fermentation, the culture temperature was 35°C, the dissolved oxygen level was maintained at 20% by adjusting the stirring speed and the ventilation rate, and the dissolved oxygen level was kept at 20% by automatic flow of ammonia water. The pH value is maintained at 6.8 (0-24 h), 6.5 (24-48 h); and the residual sugar is controlled at not less than 1.0% by feeding a glucose solution with a concentration of 100g / L, and the fermentation is stopped at 48h to obtain grain Amino acid fermentation broth; wherein, the fermentation medium is (mass percentage): glucose 9%, molasses 2%, corn steep liquor 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01% , pH6.8;

[003...

Embodiment 2

[0033] An environmentally friendly process for preparing monosodium glutamate, which comprises the steps of:

[0034] Corynebacterium glutamicum seed liquid (1 × 10 8 cfu / ml) according to the inoculum amount of 8%, inoculated in the fermenter with fermentation medium for fermentation, the culture temperature was 35°C, the dissolved oxygen level was maintained at 20% by adjusting the stirring speed and the ventilation rate, and the dissolved oxygen level was kept at 20% by automatic flow of ammonia water. The pH value is maintained at 6.8 (0-24 h), 6.5 (24-48 h); and the residual sugar is controlled at not less than 1.0% by feeding a glucose solution with a concentration of 100g / L, and the fermentation is stopped at 48h to obtain grain Amino acid fermentation broth; wherein, the fermentation medium is (mass percentage): glucose 9%, molasses 2%, corn steep liquor 3%, urea 0.5%, ferrous sulfate 0.01%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01% , pH6.8;

[003...

Embodiment 3

[0053] Comparative example 1-2 and the comparative test of the amount of acid produced in Example 1, taking the mixed solution A with a volume of 100 L as an example, the content of glutamic acid was detected, see Table 1 for details:

[0054] Table 1

[0055] group

[0056] As shown in Table 1, compared with Comparative Examples 1-3, the output of glutamic acid in Example 1 of the present invention is 1.15, 1.05 and 1.09 times that of Comparative Examples 1-3 respectively, suggesting that the treatment method of the present invention can effectively improve glutamic acid. Acid production.

[0057] 2. Effect of heat treatment temperature on acid production (g / L):

[0058] Set the temperature at 40°C, 45°C, 50°C, 55°C and 60°C respectively, the effect on the glutamic acid production of the strain under temperature gradient conditions, and the rest of the operation steps are the same as in Example 1. The specific results are shown in Table 2:

[0059] Table 2

[00...

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Abstract

The invention belongs to the technical field of monosodium glutamate preparation, and discloses an environment-friendly process for preparing monosodium glutamate. The process includes the following steps: step (1) preparing a glutamic acid fermentation liquid; step (2) reusing strains; and step (3) preparing the monosodium glutamate. According to the method, the yield of the monosodium glutamateis improved by preforming acid regeneration on waste thalli.

Description

technical field [0001] The invention belongs to the technical field of monosodium glutamate preparation, and specifically provides an environmentally friendly process for preparing monosodium glutamate. Background technique [0002] Monosodium glutamate, the scientific name is sodium glutamate, and the chemical name is monosodium α-aminoglutaric acid. It is a salt formed by sodium ions and glutamate ions. Among them, glutamic acid is an amino acid and sodium is a metal element. . The main ingredient of seasoning MSG commonly used in life is sodium glutamate. Monosodium glutamate is a commonly used flavoring agent in daily life, which can increase the umami taste of food and help improve the digestibility of food by the human body. In addition, sodium glutamate has very important functions and is widely used in food, medicine, industry and agriculture. [0003] At present, the main method of preparing monosodium glutamate is to adopt microbial fermentation technology. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12N1/20C12N1/38C12R1/15
Inventor 卢松伏广好徐淑伟周豹李文全吴明殷高雷
Owner 卢松
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