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A kind of microbial marker and application thereof for assessing the risk of type 2 diabetes

A diabetes and microorganism technology, applied in the field of microorganisms, can solve the problems of complex metagenomic sequencing process, high time cost, low specificity of markers, etc., and achieve the effect of predicting disease risk, high sensitivity and good specificity

Active Publication Date: 2020-05-05
SHANGHAI BIOTECAN PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the metagenomic sequencing process is complicated and time-consuming, and the sequencing targets many bacterial genera, and the specificity of markers is not high

Method used

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  • A kind of microbial marker and application thereof for assessing the risk of type 2 diabetes
  • A kind of microbial marker and application thereof for assessing the risk of type 2 diabetes
  • A kind of microbial marker and application thereof for assessing the risk of type 2 diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 Determination of relative content of microbial markers

[0054] 1. DNA extraction of intestinal microbial markers

[0055] method of extraction:

[0056] (1) Bacteria enrichment step:

[0057] Weigh 0.2 g of feces sample, add it to 1 ml of PBS (or normal saline), shake vigorously and then centrifuge at 500 rpm for 5 minutes to collect the supernatant; repeat this step twice; centrifuge all the collected supernatant at 5000 rpm for 10 minutes, discard supernatant.

[0058] (2) Pre-extraction treatment steps (note: if there is precipitation in the bacterial digestive juice, it can be re-dissolved in a water bath at 37°C, and shake well before use):

[0059] Gram-positive bacteria and unknown bacteria extraction pre-treatment steps: Add 180 μl lysozyme solution and 20 μl proteinase K solution to the above-mentioned different sample types of processed products, vortex and mix well, and then bathe in water at 50°C for more than 30 minutes (water bath time is ...

Embodiment 2

[0078] Embodiment 2 model analysis

[0079] Bifidobacterium, Prevotella, Faecalibaterium prausnitzii, Roseburia, Enterococcus and Veillonella were selected as assays For the combination of markers, the relative content of the detected six kinds of bacteria is used as an input variable, and the logistic regression model is used to analyze the risk of type II diabetes, and the best regression model is trained.

Embodiment 3

[0081] Compared with Example 2, except that the input variable is the relative content of Prevotella (Prevotella), Praustridium prausnitzii (Faecalibaterium prausnitzii), Roseburia (Roseburia) and Enterococcus (Enterococcus), other conditions are the same Example 2.

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Abstract

The invention provides a microbial marker for assessing the risk of type II diabetes and application of the microbial marker. The microbial marker comprises a combination of any two or more of bifidobacterium, prevotella, clostridium, roseburia, enterococcus and veillonella. The microbial marker has high sensitivity in predication of the risk of the type II diabetes; a risk warning is given through model calculation only after the relative content of the microbial marker is acquired; the microbial marker can be used for auxiliary diagnosis to guide adjustment of the intestinal microbial environment, and accordingly, the risk of suffering from diabetes is reduced.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a microbial marker and its application, in particular to a microbial marker for assessing the risk of type II diabetes and its application. Background technique [0002] Type II diabetes is an endocrine and metabolic disease based on insulin resistance and insufficient insulin secretion. Its pathogenesis is complex and may be related to genes, age, overweight or obesity, and unhealthy lifestyle. The incidence of type II diabetes is increasing year by year worldwide, and has become a serious public health problem. The "Guidelines for the Prevention and Treatment of Type 2 Diabetes in China" (2017 Edition) pointed out that the prevalence of type 2 diabetes in Chinese adults is 10.9%, and the incidence is younger: the prevalence rate under 40 years old is as high as 5.9%. Diabetes and its complications, including heart disease, kidney failure, and stroke, seriously endanger human life a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04G16H50/30G16B20/00G16B25/00C12R1/01C12R1/145
Inventor 李翠珍许骋王丽君甄林青高军晖龚建兵
Owner SHANGHAI BIOTECAN PHARMA
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