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Test strip for testing residual spiramycin

A technology of spiramycin and test strips, which is applied in the field of colloidal gold enzyme-linked immunosorbent rapid detection, can solve the problems of residue, consumer and environmental impact, and achieve the effect of low cost, simple storage and high sensitivity

Inactive Publication Date: 2019-05-28
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a veterinary drug, if the dosage and withdrawal period are not well controlled, residues will appear in the final products such as tissue and milk of edible live animals, thus causing adverse effects on consumers and the environment who eat such products

Method used

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  • Test strip for testing residual spiramycin
  • Test strip for testing residual spiramycin
  • Test strip for testing residual spiramycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of spiramycin detection test paper card

[0042] 1. Hapten synthesis

[0043] Weigh 5g of spiramycin and 6-8g of succinic anhydride.

[0044] (1) Dissolve 5g of spiramycin in an appropriate amount of pyridine, add 6-8g of succinic anhydride, and react at 75°C for 6-12h;

[0045] (2) TLC thin-layer plate detection reaction, until there is no raw material or the raw material point is very shallow, stop the reaction;

[0046] (3) Silica gel column purification and concentration to obtain the product, which is a hapten, and 2.3 g of the product was obtained, with a yield of 46%. The reaction technical route is as follows:

[0047]

[0048] The molecular structural formula of the hapten obtained by the technical route is shown in Formula I:

[0049] (Formula I).

[0050] 2. Synthesis and identification of spiramycin hapten-carrier protein conjugates

[0051] (1) Synthesis of spiramycin hapten-ovalbumin conjugate

[0052]35 mg of spiramycin ha...

Embodiment 2

[0076] Example 2 Detection of Spiramycin Residues in Samples

[0077] 1. Sample pretreatment

[0078] Weigh 2.0±0.05g of homogenized tissue and feed samples into a centrifuge tube, add 1mL of deionized water, and tightly cap the bottle. Shake for 5 minutes, draw more than three drops of the solution and pour it into a 1.5mL centrifuge tube. If there is obvious yellow turbidity, please centrifuge, and then use the supernatant as the sample solution to be tested.

[0079] 2, detect with the test paper card of the present invention

[0080] Use a dropper to drop 3 drops of sample into the hole of the reagent card, and observe the result after 5-8 minutes. If it exceeds 10 minutes, the test result of the sample will be invalid.

[0081] 3. Analysis of test results

[0082] When the concentration of spiramycin in the sample is higher than 5ng / mL, the colloidal gold antibody will fully bind to spiramycin, so that no red band will appear in the (T) area because the competition r...

Embodiment 3

[0083] Example 3 Example of sample detection

[0084] Take 20 samples each of pork, chicken, premix and batch materials and 20 samples of various negative samples with known spiramycin residue concentration greater than 5ng / g, and calculate the negative and positive rates. The results are shown in Table 1-Table 4.

[0085] batch

Positive Pork Samples (20)

Negative Pork Samples (20)

1

20 positives

20 negative

2

20 positives

20 negative

3

20 positives

20 negative

[0086] batch

Positive Chicken Samples (20)

Negative Chicken Samples (20)

1

20 positives

19 negative, 1 positive

2

20 positives

20 negative

3

20 positives

19 negative, 1 positive

[0087] batch

Positive premix samples (20 parts)

Negative premix samples (20 parts)

1

20 positives

20 negative

2

20 positives

19 negative, 1 positive

3

20 posit...

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Abstract

The invention provides an immune colloidal gold test paper card for testing spiramycin. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water absorption pad and aPVC sheet, the reaction membrane has a testing area coated with a spiramycin hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, a conjugate releasing pad iscoated with a monoclonal spiramycin antibody-colloidal gold marker. The invention further provides a method utilizing the above-mentioned test paper card for testing spiramycin. The method comprises the steps that firstly, a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the testing result is analyzed. The test paper card can be used for testing theamount of residual spiramycin medicine in animal-derived food such as chicken and feed, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting the requirements for screening a large number of samples.

Description

technical field [0001] The invention relates to a rapid detection of colloidal gold ELISA, in particular to a colloidal gold test strip for detecting spiramycin drug residues, which can realize rapid detection of spiramycin drug residues. Background technique [0002] Spiramycin (SPM for short) is a macrocyclic lactone antibiotic containing three components and three sugar groups, namely mycosyl, fulrasam, and mycosyl. Spiramycin is a macrolide antibiotic, which has antibacterial effect on Staphylococcus, Streptococcus pyogenes, Streptococcus pneumoniae, etc., and has bactericidal effect at high concentrations. The targeted flora is mainly bacteria, and it does not have the antibacterial effect on eukaryotic microorganisms. Further studies have shown that low-dose macrolides also have a good growth-promoting effect, so macrolides are also an important drug additive in animal husbandry production, and even some macrolides have been Become a special drug for livestock and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577G01N33/544
Inventor 何方洋万宇平冯才伟扶胜袁光宇罗维超钟新敏吴紫洁陈吉香
Owner 贵州勤邦食品安全科学技术有限公司
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