Bacillus amyloliquefaciens y1711, culture method and application thereof

A technology of amylolytic spores and Y1711, which is applied in the field of microorganisms and can solve problems such as inability to promote the growth of beneficial green algae

Active Publication Date: 2022-05-27
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Bacillus amyloliquefaciens disclosed in patent CN106479921A can degrade ammonia nitrogen and nitrite in aquaculture water, it cannot promote the growth of beneficial green algae

Method used

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  • Bacillus amyloliquefaciens y1711, culture method and application thereof
  • Bacillus amyloliquefaciens y1711, culture method and application thereof
  • Bacillus amyloliquefaciens y1711, culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and screening of Bacillus amyloliquefaciens Y1711

[0021] Sampling: Select 3 different sampling points in the high-ammonia-nitrogen culture pond to take the sediment, put it into a sterile ziplock bag, seal it, and bring it back.

[0022] Separation: Dilute the bottom mud with sterile water at a ratio of 1g:10mL, and after treatment in a water bath at 80°C for 10min, draw 200μl of bacterial liquid and spread it on a starch medium plate, and cultivate at 37°C.

[0023] Purification: After 24 hours of plate culture, pick the strains that produce hydrolysis circles and streak them on the nutrient agar medium, and then select single bacteria with irregular colony edges and dry and wrinkled surfaces to continue streaking and purifying until a pure culture is obtained.

[0024] Screening: The purified strains were inoculated on the starch medium plate again, and the colony diameter (C) and the hydrolysis circle diameter (H) were measured. The strain with...

Embodiment 2

[0025] Example 2 Identification of Bacillus amyloliquefaciens Y1711

[0026] 1. Morphological identification and detection of physiological and biochemical characteristics

[0027] Bacillus amyloliquefaciens Y1711 was inoculated on a nutrient agar plate, cultured at 37°C for 24 hours, and the colony growth was observed. Physiological and biochemical properties were detected according to Berger's Handbook of Bacteria.

[0028] On the nutrient agar plate, the edges of the strains are irregular, the surface is dry and wrinkled; Gram-positive, the cells are rod-shaped, the spores are mesenchymal, and the cysts are enlarged. The strain nitrate test, contact enzyme test, starch hydrolysis, D mannitol acid production test were all positive.

[0029] 2. Molecular biological identification

[0030] A single colony of Bacillus amyloliquefaciens Y1711 was picked and inoculated into a nutrient broth medium at 37°C and 200rpm for 6h shaking culture, and the bacterial genome was extracte...

Embodiment 3

[0035] Example 3 Determination of laboratory water purification function of Bacillus amyloliquefaciens Y1711

[0036] The simulated aquaculture water is prepared indoors. The simulated aquaculture water is prepared by using shrimp bait soaking solution, ammonium nitrate, dipotassium hydrogen phosphate, and sodium nitrite, and then adjust the salinity to 15 and pH 7.8±0.5 with sea crystal. The total content of ammonia nitrogen in the simulated aquaculture water was 7.79 mg / L, and the total content of nitrite was 1.03 mg / L.

[0037] Add Bacillus amyloliquefaciens Y1711 bacterial liquid to the simulated breeding water, so that the bacterial content in it is 10 6 cfu / mu, before adding the bacteria solution and on the 1st, 2nd, 3rd, 5th, and 7th days after adding the bacteria solution, take water to measure the ammonia nitrogen and nitrite content in the water. The data are shown in Table 1 below.

[0038] The bacterial culture method of Bacillus amyloliquefaciens Y1711 is as foll...

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PUM

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus amyloliquefaciens Y1711, a cultivation method and an application thereof. The Bacillus amyloliquefaciens Y1711 is Bacillus amyloliquefaciens Y1711, and its preservation number in the China Type Culture Collection Center is CCTCC NO:M 2018356, the deposit date is June 11, 2018. The Bacillus amyloliquefaciens Y1711 disclosed by the present invention can significantly reduce ammonia nitrogen and nitrite in aquaculture water, significantly increase the growth rate of beneficial green algae, thereby forming a virtuous cycle, and is harmless to humans and animals, and can be scaled up Production is used to improve the aquaculture environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus amyloliquefaciens Y1711, a culture method and applications thereof. Background technique [0002] With the continuous increase of aquaculture area in my country, the discharge of aquaculture wastewater and the accumulation of organic sludge formed by residual bait, feces, and biological debris have made the organic pollution of aquaculture water continue to increase, and the quality of aquaculture ecological environment has deteriorated year by year. The water quality of some aquaculture water bodies is seriously aging, and the aquaculture parameters such as ammonia nitrogen and nitrite in the water bodies continue to deteriorate; Bacillus is a common probiotic that uses microorganisms to repair the breeding environment. Among them, Bacillus amyloliquefaciens secretes a large amount of amylase, protease and lipase during the reproduction process, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/38C12N1/12C02F3/34C12R1/07C02F101/16
CPCY02W10/10
Inventor 王启烁吴晓娇张善夫张景龙孙家凯
Owner INST OF AQUATIC LIFE ACAD SINICA
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