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A kind of preparation method of allogeneic platelet-rich gelatin factor

A platelet-rich, gel factor technology, used in prostheses, fluid delivery, medical raw materials derived from mammals, etc., can solve the problem of low platelet-rich factor concentration, reduce the risk of infection, optimize centrifugal force and centrifugation time, Pain-reducing effect

Active Publication Date: 2021-02-02
HEILONGJIANG HENGSHENG STEM CELL ENG +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In view of the above-mentioned prior art, in order to solve the limitation that the platelet-rich factor extracted and prepared in the prior art is only used for autologous treatment, and to solve the defect of the low concentration of the platelet-rich factor extracted and prepared in the prior art, the present invention provides a Preparation method of allogeneic platelet-rich gel factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of platelet-rich gel factor

[0027] Proceed as follows:

[0028] (1) Cord blood collection:

[0029] 1) After the fetus is delivered, the midwife clamps the umbilical cord with two vascular clamps, cuts the umbilical cord with scissors from between the two clamps, and takes the newborn away.

[0030] 2) Sampling the umbilical cord immediately for disinfection: lay a sterile towel on the bottom, place it on the fixed sterile towel, and hang it down naturally; use sterile gauze to scrub evenly from bottom to top; use 2% iodophor gauze from top to bottom Disinfect evenly three times.

[0031] 3) Fix the umbilical cord with the left hand and prepare the sample book for puncture; at the upper end of the Venturi forceps 2 to 3 cm, make the needle tip and the umbilical cord plane form an angle of 15 degrees, and quickly penetrate the umbilical vein with the inclined surface downward, stretch the umbilical cord, and let the sample collection bag natural...

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Abstract

The invention discloses a preparation method of allogeneic platelet-rich gelatin factor: (1) collection of umbilical cord blood; (2) first centrifugation: centrifuging the blood sample with the centrifugation parameter of 1500r / min for 10 minutes; absorbing the supernatant after centrifugation 0.5-1 mm above the red blood cell layer, the middle layer, and the upper end of the erythrocyte layer, and perform the second centrifugation; (3) The second centrifugation: the centrifugation parameter is 2800r / min, 10min; Platelet-rich factor; (4) Mix platelet-rich factor and calcium chloride-bovine thrombin solution at a volume ratio of 1:8-10, mix well, and let stand for more than 2 minutes to obtain platelet-rich factor gel. The allogeneic platelet-rich gel factor prepared by the method of the present invention can be used for allogeneic treatment, and the concentration can reach 9 times of the original whole blood concentration, can be used for acute and chronic wound repair, and can be used as a composite artificial bone for the treatment of fracture nonunion .

Description

technical field [0001] The invention relates to a preparation method of allogeneic platelet-rich gelatin factor, which belongs to the technical field of blood products. Background technique [0002] Platelet-rich factor is a platelet concentrate obtained by gradient centrifugation and separation of autologous whole blood, and is rich in platelets. The normal platelet concentration in human blood is 100,000 μl to 350,000 μl, with an average of 200,000 μl. The platelet concentration in platelet-rich factor is generally 3 to 10 times that of whole blood. When platelets are activated, they can release a variety of growth factors, which play an important role in promoting the proliferation, growth, differentiation and tissue formation of bone cells and osteoblasts. Since Marx et al first used platelet-rich factor composite bone graft to repair mandibular defects in 1998, platelet-rich factor has been gradually applied to tissue repair in the fields of stomatology, plastic surger...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/19A61K9/06A61P17/02A61P19/08A61L27/54
Inventor 初相伍王峰徐峰波孙禄伟张晓东
Owner HEILONGJIANG HENGSHENG STEM CELL ENG
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