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Micromolecule DNA purification reagent

A small molecule and reagent technology, applied in the field of small molecule DNA purification reagents, can solve the problem of low efficiency of small molecule DNA purification

Pending Publication Date: 2019-06-18
WUHAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is: how to provide a small molecule DNA purification reagent to solve the problem of low purification efficiency of small molecule DNA by existing PCR purification kits and DNA gel extraction kits

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Embodiment Construction

[0024] 2.2.1) Reagent formula and preparation method of the two solutions

[0025] Reagents for efficient purification of small molecule DNA include saturated sodium iodide solution (NaI) and DNA washing solution.

[0026] a) Formula and preparation of saturated sodium iodide solution

[0027] Recipe: NaI,Na 2 SO 3 ,H 2 0.

[0028] Preparation method:

[0029] (1) Weigh 210 grams of NaI in an electronic balance, and place the weighed NaI in a 500 milliliter beaker;

[0030] (2) Take 100 ml of distilled water, heat it in a water bath at 50°C, add NaI into the beaker, stir well

[0031] Dissolve NaI to the greatest extent until there is still a small amount of NaI solid that has not been completely dissolved, and stand at room temperature for 1 hour;

[0032] (3) Adjust the pH value of the solution to 6.0 with concentrated hydrochloric acid, let it stand overnight, and transfer the supernatant to a sterile reagent bottle;

[0033] (4) Add 0.5 grams of sodium sulfite (Na ...

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Abstract

The invention discloses a micromolecule DNA purification reagent. The micromolecule DNA purification reagent is prepared from two independent reagents of the reagent A and the reagent B, wherein the reagent A is a saturated sodium iodide solution (pH 6.0) and contains Na2SO3 with the mass concentration of 0.5%, and the reagent B is a DNA washing solution prepared from 20 mM of Tris.HCl with the pHof 7.2, 50 mM of KCl and 85% ethyl alcohol. The micromolecule DNA purification reagent can simultaneously replace reagents in a PCR product purification kit and a DNA glue extraction kit for purifying DNA or extracting DNA from sepharose gel, particularly has a great purification effect on micromolecule DNA, and has great application value and market prospects.

Description

technical field [0001] The invention belongs to the field of chemical reagents, in particular to a small molecule DNA purification reagent. Background technique [0002] Gene cloning is one of the most frequently used techniques in modern molecular biology, widely used in agriculture, medicine, biological research, biopharmaceuticals and other fields. Gene cloning is the process of ligating the digested target gene (or DNA fragment) with the vector digested by the same enzyme under the action of DNA ligase, and then introducing the recombinant DNA into a bacterial host for replication to obtain a large amount of recombinant DNA. This process includes a series of DNA manipulation techniques, one of the key steps is the purification of DNA, which plays a vital role in the efficiency of gene cloning. For example, the DNA obtained by the polymerase chain reaction (PCR) needs to be purified, and the digested product also needs to be purified after agarose gel electrophoresis. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 邓文生彭飞霞吴自辉
Owner WUHAN UNIV OF SCI & TECH
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