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Self-healing hydrogel entrapping exosome as well as preparation method and application thereof

An exosome, self-healing technology, applied in the field of medical engineering, can solve the problems of gangrene, increase the risk of amputation and mortality, reduce the quality of life of patients, etc. injectable effect

Active Publication Date: 2019-06-21
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, some complications caused by diabetes have brought great suffering to patients, thereby reducing the quality of life of patients.
For example, delayed wound healing in diabetic patients is one of the most challenging problems as it leads to gangrene, increased risk of amputation and mortality
At present, the main methods used include suturing in physical methods to reduce the area of ​​the wound, or injecting cells or growth factors to accelerate wound healing, but there is a problem of high fluidity and low efficiency.

Method used

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  • Self-healing hydrogel entrapping exosome as well as preparation method and application thereof
  • Self-healing hydrogel entrapping exosome as well as preparation method and application thereof
  • Self-healing hydrogel entrapping exosome as well as preparation method and application thereof

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Experimental program
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preparation example Construction

[0050]

[0051] Such as figure 1 As shown, the preparation method of the self-healing hydrogel encapsulating exosomes of the present invention comprises the following steps:

[0052] The phosphate buffer solution of formhylated methylcellulose (MC-CHO) and the phosphate buffer solution of pegylated chitosan (chitosan-g-PEG) were mixed to obtain a mixture; the placental mesenchymal stem cells The exosomes were added to the mixture and stirred evenly, and the transformation from solution to gel was realized within 5 minutes, that is, the self-healing hydrogel containing exosomes was obtained;

[0053]

[0054] Wherein, the volume ratio of the phosphate buffer saline of aldehylated methylcellulose and the phosphate buffer saline of pegylated chitosan is 1:1; the number of placental mesenchymal stem cell exosomes is 1× 10 10 individual / mL.

[0055] The content of formylated methylcellulose in the phosphate buffer of formylated methylcellulose can be 10 ± 1wt%, preferably 1...

Embodiment 1

[0087] The preparation method of the self-healing hydrogel encapsulating exosomes of this embodiment comprises the following steps:

[0088] (1) Dissolve 5g of methylcellulose (MC) in N,N-dimethylformamide to obtain the first mixture, 0.149g, 1mmol of p-aldehyde benzoic acid, 0.178g, 1.2mmol of dicyclohexylcarbodi Imine (DCC) and 14.66mg, 0.12mmol 4-dimethylaminopyridine (DMAP) were dissolved in 70mL of the first mixture, and the system was subjected to three rounds of freezing-vacuumizing-argon filling-freezing, and magnetically stirred at room temperature React for 12 hours to obtain the second mixture, which was precipitated in frozen ether, redissolved in N,N-dimethylformamide, dialyzed for 3 days and lyophilized to constant weight to obtain white solid formylated methylcellulose prime (MC-CHO);

[0089] (2) Dissolve 5.2g, 1.04mmol methyl polyethylene glycol (mPEG), 1.56g, 1.56mmol succinic anhydride, 0.1442mL, 1.04mmol triethylamine and 127mg, 1.04mmol 4-dimethylaminopyr...

Embodiment 2

[0094] The preparation method of the self-healing hydrogel encapsulating exosomes of this embodiment comprises the following steps:

[0095] (1) Dissolve 5g of methylcellulose (MC) in N,N-dimethylformamide to obtain the first mixture, 0.149g, 1mmol of p-aldehyde benzoic acid, 0.178g, 1.2mmol of dicyclohexylcarbodi Imine (DCC) and 14.66mg, 0.12mmol 4-dimethylaminopyridine (DMAP) were dissolved in 70mL of the first mixture, and the system was subjected to three rounds of freezing-vacuumizing-argon filling-freezing, and magnetically stirred at room temperature After 24 hours of reaction, the second mixture was obtained. After the second mixture was precipitated in frozen cyclohexane, it was redissolved in N,N-dimethylformamide, dialyzed for 2 days and lyophilized to constant weight to obtain a white solid formaldehyde Cellulose-based (MC-CHO);

[0096] (2) Dissolve 5.2g, 1.04mmol methyl polyethylene glycol (mPEG), 1.56g, 1.56mmol succinic anhydride, 0.1442mL, 1.04mmol triethylam...

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Abstract

The invention provides self-healing hydrogel entrapping exosome as well as a preparation method and application thereof. The preparation method comprises the following steps: performing reaction modification on methylcellulose and p-aldehyde benzoic acid to obtain aldehyde methyl cellulose; performing amidation reaction on chitosan and methyl polyethylene glycol to obtain pegylated chitosan whichis dissoluble under the neutral condition; and separating from placental mesenchymal stem cell supernatant by a centrifugal method to obtain the exosome, mixing the exosome, the aldehyde methyl cellulose and the pegylated chitosan to form a crosslinked network structure of dynamic reversible Schiff base through the aldehyde on the aldehyde methyl cellulose and the amino in the pegylated chitosan molecule. The self-healing hydrogel can be applied to skin trauma repair at exercise site. The gel has antibacterial property and the exosome shows sustained-release effect in the gel, so that the self-healing hydrogel entrapping the exosome has high biocompatibility, high self-healing property and degradation rate.

Description

technical field [0001] The invention belongs to the technical field of medical engineering, in particular to polymer materials in intelligent bionic materials, and more specifically relates to a self-healing hydrogel containing exosomes and a preparation method and application thereof. Background technique [0002] In recent years, with the improvement of living standards, people pay more and more attention to medicine and health. From 2006 to 2016, the absolute number of deaths and years of life lost (YLLs) due to diabetes increased by 31.1% and 25.3%, respectively. At the same time, some complications caused by diabetes have brought great suffering to patients, thus reducing the quality of life of patients. Delayed wound healing in diabetic patients, for example, is one of the most challenging problems as it can lead to gangrene, increasing the risk of amputation and mortality. At present, the main methods used include suturing in physical methods to reduce the area of ​...

Claims

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Application Information

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IPC IPC(8): C08L1/28C08L5/08C08J3/075C08B11/20C08G65/332C08B37/08C08G81/00A61L26/00
Inventor 袁伟忠王春堯汪芮谢晓云刘阳
Owner TONGJI UNIV
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