A compound bactericide of perylenequinone compound
A technology of compounding fungicides and compounds, applied in the directions of fungicides, biocides, biocides, etc., can solve the problem of high toxicity of traditional pesticides, and achieve the effect of improving disease resistance, wide bactericidal spectrum, and promoting absorption and conduction.
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Embodiment 1
[0017] Study on the effect of the mass ratio of various perylenequinone compounds on the antibacterial activity of compound fungicides
[0018] 1. Experimental materials and methods
[0019] 1.1 Source of material
[0020] The representative pathogenic bacteria Fusarium solani (Fusarium solani) and Bacillus subtilis (Bacillus subtilis) in root rot are both preserved in the Department of Biopharmaceuticals, Binzhou University. PDA solid medium, PD liquid medium, common broth liquid medium and common broth solid medium, etc. are prepared according to conventional methods, and then sterilized.
[0021] 1.2 Preparation of paper sheets
[0022] Soak the filter paper piece (8 mm) in the dimethyl sulfoxide solution containing different perylenequinone compounds for a while, then store it in the dark for future use.
[0023] 1.3 Measurement of compound fungicide activity
[0024] According to the aseptic operation procedures, draw 100 μL of the test bacterial suspension respective...
Embodiment 2
[0029] The optimization experiment results of the metal ions and concentration of the factors related to the antibacterial activity of compound fungicides
[0030] 1. Experimental materials and methods
[0031] 1.1 Source of material
[0032] The representative pathogenic bacteria Fusarium solani (Fusarium solani) and Bacillus subtilis (Bacillus subtilis) in root rot are both preserved in the Department of Biopharmaceuticals, Binzhou University.
[0033] PDA solid medium, PD liquid medium, common broth liquid medium and common broth solid medium, etc. are prepared according to conventional methods, and then sterilized.
[0034] 1.2 Preparation of paper sheets
[0035] Soak the filter paper piece (8 mm) in the dimethyl sulfoxide solution containing different perylenequinone compounds for a while, then store it in the dark for future use.
[0036] 1.3 Measurement of compound fungicide activity
[0037] According to the aseptic operation procedures, draw 100 μL of the test ba...
Embodiment 3
[0054] field experiment
[0055] A compound fungicide containing perylenequinone compounds, including hypocrellin A, hypocrellin B, and estrocystin in a mass ratio of 9:1.5:3, 0.055mol / L Ca 2+ , 0.035mol / L Mg 2+ , 0.06g / L extract of the ethyl acetate part of bamboo yellow fungus. The application method is to mix and stir the compound fungicide evenly, then dissolve it in water and dilute it. The effective content of the compound fungicide is 3%, and the fungicide is directly sprayed on the common wheat field, which can effectively prevent and control various fungicides in the wheat field. plant pathogenic bacteria, and the sterilization rate is controlled above 98%.
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