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Rooibos tea and content determination method of effective components in product of Rooibos tea

The technology of a line-leaf gorse and determination method, which is applied in the field of food quality control, can solve problems such as inaccurate and comprehensive quality assessment, single detection method measurement results, and complicated extraction operations, and achieves simplified pretreatment steps and extraction methods Simple, highly specific effects

Inactive Publication Date: 2019-06-25
完美(广东)日用品有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is that the detection method of the prior art has a single measurement result, complex extraction operation, low resolution, poor peak shape, long running time, and cannot accurately and comprehensively evaluate its quality. A fast, accurate, convenient, and cheap method for determining the content of isoorientin, orientin and aspartin in gorse laceae and gorse laceae extracts and gorse laceae tea, and then comprehensively and Accurately control the quality of gorse and its products

Method used

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  • Rooibos tea and content determination method of effective components in product of Rooibos tea
  • Rooibos tea and content determination method of effective components in product of Rooibos tea
  • Rooibos tea and content determination method of effective components in product of Rooibos tea

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preparation example Construction

[0048] Preparation of mixed reference substance solution: as shown in Table 1, accurately weigh the appropriate amount of isoorientin reference substance, orientin reference substance and aspartame reference substance, add 60vt% ethanol solution containing 0.2wt% vitamin C to dissolve Prepare the mixed standard stock solution containing 476.016 μg isoorientin reference substance and 444.074 μg orientin reference substance per 1 ml and the aspartame standard stock solution B containing 182.662 μg aspartame reference substance respectively, and then respectively Precisely draw 1ml of each of the two groups of standard stock solutions, put them in a 10ml measuring bottle and mix them, then add 60vt% ethanol solution containing 0.2wt% vitamin C to dilute to the mark, shake well, and then get the mixed reference solution;

[0049] Preparation of isoorientin reference substance solution: Accurately weigh an appropriate amount of isoorientin reference substance, add 60vt% ethanol solu...

Embodiment 1

[0128] The method for assaying the content of active ingredients in the Gorse fruticosa extract described in the present embodiment may further comprise the steps:

[0129] Preparation of mixed reference substance solution: Accurately weigh the appropriate amount of isoorientin reference substance, orientin reference substance and aspartame reference substance, add 60vt% ethanol solution to dissolve, and make 476.016μg isoorientin reference substance per 1ml respectively product and mixed standard stock solution containing 444.074 μg orientin reference substance and aspartame standard stock solution B containing 182.662 μg aspartame reference substance, and then accurately draw 1ml of each of the two sets of standard stock solutions and place them in a 10ml volume Mix in the bottle, then add 60vt% ethanol solution to dilute to the volume, shake well to get the mixed reference solution;

[0130] Preparation of the test solution: Get 0.2 g of the fermented gorse extract, accurat...

Embodiment 2

[0135] The method for assaying the content of active ingredients in the Gorse fruticosa extract described in the present embodiment may further comprise the steps:

[0136] Preparation of mixed reference substance solution: Accurately weigh the appropriate amount of isoorientin reference substance, orientin reference substance and aspartame reference substance, add 60vt% ethanol solution containing 0.2wt% vitamin C to dissolve and make each 1ml containing 476.016 μg isoorientin reference substance and mixed standard stock solution containing 444.074 μg orientin reference substance and aspartame standard stock solution B containing 182.662 μg aspartame reference substance, and then accurately pipette two sets of standard stock solutions 1ml each, put in a 10ml measuring bottle and mix, then add 60vt% ethanol solution containing 0.2wt% vitamin C to dilute to the volume, shake well to obtain the mixed reference solution;

[0137] Preparation of the test solution: Take 0.2 g of th...

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Abstract

The present invention belongs to the technical field of food quality control, and especially provides a Rooibos tea and a content determination method of effective components in a product of Rooibos tea. The method comprises the following content determination steps of isoorientin: A: content determination of isoorientin; preparation of an isoorientin reference solution; preparation of a test solution; and chromatographic condition and system suitability test: performing determination according to the high-performance liquid chromatography by employing a Waters Symmetry C18 (4.6mm*250mm, 5[mu]m) chromatographic column or a Diamonsil C18 (4.6mm*250mm, 5[mu]m) chromatographic column, taking acetonitrile as a mobile phase A, taking an acetum with a volume concentration of 0.8-1.5% or formic acid with a volume concentration of 0.1-0.5% as a mobile phase B, performing chromatographic analysis according to a gradient elution program, controlling the flow speeds of the mobile phases in a range of 0.8-1.2ml / min, and controlling the detection wavelengths of the mobile phases in ranges of 275-295nm and 340-360nm, and determining and calculating the content of the isoorientin in the test solution. The Rooibos tea and the content determination method of effective components in a product of Rooibos tea are fast, accurate and convenient for determination of the content of the effective components in a product of Rooibos tea.

Description

technical field [0001] The invention belongs to the technical field of food quality control, and in particular relates to a method for measuring the content of active ingredients in gorse fruticosa and its products. Background technique [0002] Gorse linearis is a South African leguminous plant (Latin scientific name: Aspalathus Linearis (Brum.f.) R.Dahlgren), and it is a new food raw material announced by the National Health and Family Planning Commission. The extract of gorse is the water extract of gorse, and the tea of ​​gorse is a common drink containing the extract of gorse. Line-leaf gorse is rich in antioxidant flavonoids, which have a significant effect on eliminating free radicals, regulating human body functions, preventing and controlling various diseases. Line-leaf gorse does not contain caffeine and oxalic acid, and has a calming center Nervous system effect, suitable for people who suffer from allergies, headaches, sleep disorders, insomnia, nervous tension,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/74G01N30/04G01N30/86
Inventor 梁淑明李晓敏郁晓艺欧阳道福陈晶吴键梅卜闪闪曾瑜魏毅凡
Owner 完美(广东)日用品有限公司
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