Virus-free potato minituber propagation technology

Abstract

The invention relates to the field of plant cultivation, in particular to a virus-free potato minituber propagation technology. The virus-free potato minituber propagation technology comprises the following steps that seedlings subjected to tissue culture are cut into segments and are subjected to cuttage in a culture system containing a matrix and a sugar-free culture solution for illumination culture, ventilation is carried out in the illumination cultivation process, gas containing carbon dioxide is introduced into the culture system in the ventilation process, so that potato test-tube plantlets can grow continuously and healthily under the sugar-free condition, the seedling pollution probability is lowered, and the production cost is saved; and then seedlings with the height of 10-12 cm are directly transferred into a greenhouse along withe hole trays for minituber production so as to form a one-step minituber propagation technology.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a technique for breeding virus-free potato miniature tubers. Background technique [0002] Potato (Solanum tuberosum L.) is the fourth largest food crop in my country. Because of its short growth period, high yield, strong adaptability, and wide application, it has become the main crop with the fastest growing cultivated area in my country in the past 20 years. my country is a veritable potato producing country with a planting area of ​​more than 6 million hectares and an annual output of over 100 million tons, ranking first in the world. However, the potato yield per unit area is lower than the world average level, which is 2-3 times lower than that of developed countries. The main reason is determined by the reproductive characteristics of potatoes. Potatoes have a low reproductive coefficient and a large amount of seeds. The long-term seed potato propagation process lacks eff...

AI Technical Summary

Problems solved by technology

The main reason is determined by the reproductive characteristics of potatoes. The reproductive coefficient of potatoes is low and the amount of seeds used is large. The long-term seed potato propagation process lacks effective quality control and detection technology, which can easily lead to virus re-infection and seed degradation.

Breeding of virus-free miniature tubers is an important technology to solve this problem. At present, the production of miniature tubers is mostly based on test-tube plantlets, while in the prior art, sucrose or other sugar substances are used to cultivate potato tuber plants. During the process, due to the existence of sugar substances, the pollution rate is high, and when the sugar substances are used for cultivation, the culture system needs to be sealed, which in turn leads to poor ventilation, slow growth of test tube plantlets, and some abnormal forms of leaves, such as leaf surface Poor structural development, abnormal regulation of stomata, small leaves, low chlorophyll content, etc.

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