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Bletilla striata polysaccharide extract and preparation method thereof

The technology of Bletilla striata polysaccharide and extract is applied to the field of Bletilla striata polysaccharide extract and its preparation, which can solve the problems of difficulty in removing impurities such as protein and starch, and reduce the extraction purity of Bletilla striata polysaccharide, and achieves low destructiveness, low protein content and high efficiency. Effect

Active Publication Date: 2019-07-05
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the problem that impurities such as protein and starch are difficult to remove in the extraction process of bletilla striata polysaccharides in the prior art and reduce the extraction purity of bletilla striata polysaccharides, and provides a method for extracting bletilla striata polysaccharides, which can reduce the protein and starch content in the purified bletilla striata polysaccharides. Starch content, improve the purity of bletilla striata polysaccharide

Method used

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  • Bletilla striata polysaccharide extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The Bletilla striata medicinal material was crushed through a 40-mesh sieve, 50 g of the powdered Bletilla striata powder was weighed, added into 3500 mL of water under stirring, and ultrasonically extracted for 9 hours at a stirring speed of 27 r / min and an ultrasonic frequency of 28 kW, centrifuged, Filter to obtain the water extract of Bletilla striata.

[0045] Concentrate the bletilla striata water extract obtained above to a crude drug concentration of 0.1 g / mL, add absolute ethanol to 60% ethanol concentration under unidirectional stirring, let stand at 4°C for 12 hours, filter with suction, and successively wash with absolute ethanol , acetone and ether, and then vacuum-dried at 20°C to obtain the crude polysaccharide of Bletilla striata.

[0046] Add the above-mentioned Bletilla striata crude polysaccharide into deionized water to dissolve, centrifuge, take the supernatant and filter it with a microfilter (0.22 μm), take the microfiltrate at a pressure of 0.10 ...

Embodiment 2

[0048] The bletilla striata medicinal material was crushed through an 80-mesh sieve, 50 g of the pulverized bletilla striata powder was weighed, added into 4500 mL of water under stirring, and ultrasonically extracted for 5 hours at a stirring speed of 27 r / min and an ultrasonic frequency of 28 kW, centrifuged, Filter to obtain the water extract of Bletilla striata.

[0049] After concentrating the bletilla striata water extract obtained above to a crude drug concentration of 0.1 g / ml, add absolute ethanol under unidirectional stirring until the ethanol concentration is 40%, place it at 10° C. for 24 hours, filter with suction, and successively wash with absolute ethanol , acetone and ether, and then vacuum-dried at 50°C to obtain the crude polysaccharide of Bletilla striata.

[0050] Add the above-mentioned crude polysaccharide of Bletilla striata into deionized water to dissolve, centrifuge at high speed, take the supernatant and filter it with a microfilter (0.45 μm), take ...

Embodiment 3

[0052] The bletilla striata medicinal material was crushed through a 100-mesh sieve, and 50 g of the pulverized bletilla striata powder was weighed, added into 250 mL of water under stirring, and ultrasonically extracted for 0.5 hours under the conditions of a stirring speed of 27 r / min and an ultrasonic frequency of 28 kW, centrifuged, Filter to obtain the water extract of Bletilla striata.

[0053] Concentrate the bletilla striata water extract obtained above to a crude drug concentration of 0.1 g / ml, add absolute ethanol to an ethanol concentration of 80% under unidirectional stirring, place it at 30°C for 72 hours, filter with suction, and successively wash with absolute ethanol , acetone and ether, and then vacuum-dried at 40°C to obtain the crude polysaccharide of Bletilla striata.

[0054] The above-mentioned crude polysaccharide of Bletilla striata was dissolved in deionized water, centrifuged at high speed, and the supernatant was filtered with a microfilter (0.8 μm)....

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Abstract

The invention relates to the field of pharmacy, and discloses a Bletilla striata polysaccharide extract and a preparation method thereof. The preparation method comprises the following steps: crushingand sieving a Bletilla striata medicinal material to prepare Bletilla striata powder; adding the Bletilla striata powder to water, and carrying out ultrasonic extraction, centrifuging and filtering to prepare Bletilla striata water extract; concentrating the Bletilla striata water extract, adding ethanol to precipitate, taking the obtained precipitate, and washing and drying the precipitate to obtain crude Bletilla striata polysaccharides; and adding distilled water to dissolve the crude Bletilla striata polysaccharides, centrifuging the obtained solution, taking the obtained supernatant, sequentially carrying out microfiltration and ultrafiltration, concentrating the filtered supernatant, precipitating the obtained concentrate with alcohol, and taking, washing and drying the obtained precipitate in order to obtain the Bletilla striata polysaccharide extract. The ultrasonic extraction process used to destroy cell walls of the Bletilla striata powder facilitates the full dissolving ofthe Bletilla striata polysaccharides, and microfiltration-ultrafiltration combined purification is carried out based on the preparation of the crude Bletilla striata polysaccharides through an ultrasonic water extraction and alcohol precipitation technology to prepare the Bletilla striata polysaccharide extract, so the method can effectively remove proteins, starch and other impurities, and the prepared Bletilla striata polysaccharide extract has a high purity and a low protein content, and is free from starch and inactive polysaccharides.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a bletilla striata polysaccharide extract and a preparation method thereof. Background technique [0002] Bletilla striata is the dried tuber of Bletilla striata (Thunb.) Reichb.f., a perennial herbaceous plant in the family Orchidaceae. It is sticky and astringent in taste. It is an important medicine for astringent and hemostasis. It is mostly used clinically for lung and stomach bleeding. Its main medicinal part is bletilla striata gum (Bletilla striata polysaccharide), which has anti-inflammatory, blood-promoting, anti-oxidation and other medicinal effects. At the same time, Bletilla striata polysaccharide has good tissue compatibility, bioadhesion, biodegradability, non-irritating to mucous membranes, and is rich in resources, cheap and easy to obtain, and has the characteristics of bioadhesive carrier materials. Bletilla striata polysaccharide is a water-soluble heteropolysaccharid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 石森林何三民吴瑾瑾徐菲拉冯超胡锦祥陈烨来银芳
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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