Fluorescent indicator combination, fluorescent array sensor, preparation method and application thereof

Abstract

The invention discloses a fluorescent indicator combination, a fluorescent array sensor, a preparation method and application thereof. The fluorescent indicator combination includes various carbon quantum dot-metal ion complexes formed by full-color fluorescent carbon quantum dots and various metal ions under different pH conditions. The fluorescent array sensor includes the fluorescent indicator, and the sensor includes multiple groups of response points, each group of response points includes more than three independent response points respectively corresponding to excitation wavelengths of 360nm, 450nm, and 540nm, and each response point includes At least one carbon quantum dot-metal ion composite. The fluorescence array sensor of the present invention can simultaneously realize the qualitative and semi-quantitative detection of tetracyclines, quinolones, β-lactams and aminoglycosamines, etc. four major antibiotics; and its production process is simple, long storage time and detection sensitivity Preferably, the simultaneous differentiation and detection of multiple types of antibiotics can be realized.

Description

Fluorescent indicator combination, fluorescent array sensor, preparation method and application thereof technical field The present invention relates to a kind of array sensor that is used for the detection of antibiotics and distinguish, relate in particular to a kind of detection and distinguish Fluorescent indicator combination of multi-class antibiotics, multi-channel fluorescent array sensor and preparation method thereof, and its application in realizing multi-class The application of the simultaneous detection of antibiotics belongs to the technical field of food safety detection. Background technique Antibiotics are capable of killing or inhibiting the ability of bacteria to grow and have been mass-produced worldwide over the past few decades. and is used to treat various human and veterinary diseases. At present, more than the standard is found in milk, vegetables, grains, surface water and wastewater of antibiotics. Among them, three types of antibiotics ...

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Problems solved by technology

However, the biggest problem with these methods is that the cost of the required detection equipment is extremely high, and even professional operators are required to complete it, and the sample pretreatment process is complicated, etc., which are not suitable for immediate on-site detection.

[0004] At present, there are many common antibiotic detection methods, among which D.Vega (Analytical and BioanalyticalChemistry 2007, 389, 951–958.) et al. used an electrochemical method to detect four tetracycline antibiotics using electrodes improved by multi-walled carbon nanotubes, and the detection limit was 0.44μM, unable to detect and distinguish multiple antibiotics

Lu Haitao (Chromatographia 2004,60,259–264.) et al. used high-performance liquid chromatography to detect a variety of antibiotics. The detection range was between 0.21-104μM, and the sensitivity was not enough. At the same time, high-performance liquid chromatography was used , requires expensive instruments, professional operators, and complicated sample pretreatment, which is not cost-effective for practical applications

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