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Bradyrhizobium liquid culture medium and preparation and culture methods thereof

A brachyrhizobia and liquid culture medium technology is applied in the field of culture, brachyrhizobium liquid culture medium and its preparation, which can solve the problems of multiple fermentations, long fermentation time, feeding in the middle, etc., so as to achieve high bacterial density. , the preparation method is simple, and the growth efficiency is improved.

Inactive Publication Date: 2019-07-09
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of bradyrhizobium liquid culture medium and its preparation and cultivation method, solve the problems of multiple fermentations, midway feeding and long fermentation time in the cultivation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 bradyrhizobium substratum

[0022] The bradyrhizobium culture medium described in the present embodiment is made of fulvic acid, mannitol, yeast extract, K 2 HPO 4 、KH 2 PO 4 , MgSO 4 , NaCl, CaCl 2 , FeCl 3 、H 3 BO 3 、Na 2 MnO 4 and biotin dissolved in water and mixed evenly, wherein the mass concentrations of the components are 0.1g / L, 10g / L, 1g / L, 0.5g / L, 0.5g / L, 0.2g / L, 0.1g / L L, 0.04g / L, 0.03g / L, 0.025g / L, 0.025g / L, 0.1g / L, the pH of the prepared solution is 6.8.

Embodiment 2

[0023] Embodiment 2 bradyrhizobium substratum

[0024] The bradyrhizobium culture medium described in the present embodiment is made of fulvic acid, mannitol, yeast extract, K 2 HPO 4 、KH 2 PO 4 , MgSO 4 , NaCl, CaCl 2 , FeCl 3 、H 3 BO 3 、Na 2 MnO 4 and biotin dissolved in water and mixed evenly, wherein the mass concentrations of the components are 0.1g / L, 10g / L, 1g / L, 0.5g / L, 0.5g / L, 0.2g / L, 0.1g / L L, 0.04g / L, 0.03g / L, 0.025g / L, 0.025g / L, 0.1g / L, the pH of the prepared solution is 7.0.

Embodiment 3

[0025] Embodiment 3 bradyrhizobium substratum

[0026] The bradyrhizobium culture medium described in the present embodiment is made of fulvic acid, mannitol, yeast extract, K 2 HPO 4 、KH 2 PO 4 , MgSO 4 , NaCl, CaCl 2 , FeCl 3 、H 3 BO 3 、Na 2 MnO 4 and biotin dissolved in water and mixed evenly, wherein the mass concentrations of the components are 0.1g / L, 10g / L, 1g / L, 0.5g / L, 0.5g / L, 0.2g / L, 0.1g / L L, 0.04g / L, 0.03g / L, 0.025g / L, 0.025g / L, 0.1g / L, the pH of the prepared solution is 7.5.

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PUM

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Abstract

The invention discloses a bradyrhizobium liquid culture medium and preparation and culture methods thereof. The bradyrhizobium liquid culture medium is prepared from fulvic acid, mannitol, a yeast extract, K2HPO4, KH2PO4, MgSO4, NaCl, CaCl2, FeCl3, H3BO3, Na2MnO4 and biotin. The preparation method includes the step that the raw materials and water are mixed and prepared into a solution. The raw materials of the prepared culture medium for culturing bradyrhizobium have wide sources; the preparation method is easy to implement, fermentation only needs to be conducted once, and no materials needto be added halfway; on this basis, the effect of adopting the prepared culture medium to culture bradyrhizobium is better than that of adopting an ordinary culture medium for culturing, and the culture method includes the steps of activating strains, culturing seeds and conducting fermentation culture. The mycelium density of bradyrhizobium cultured by the culture medium in the same period of time is greater; on the premise of meeting a certain quantity of mycelia, the fermentation time can be shortened; therefore, the effect of the prepared culture medium on the bradyrhizobium growth efficiency is obviously improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bradyrhizobium liquid culture medium and its preparation and cultivation methods. Background technique [0002] The symbiotic nitrogen fixation system of rhizobia and leguminous plants is the biological nitrogen fixation system with the highest nitrogen fixation rate and the largest amount of nitrogen fixation in nature. Inoculation of nodulation agents has become a technical measure to increase the yield of leguminous crops in countries all over the world. This technology can increase the nitrogen content in the soil, effectively supply the nitrogen demand of legumes, and promote the increase of legumes production. YEM medium is often used for the cultivation of rhizobia in production, and its composition is: mannitol 10g / L, yeast extract 1.0g / L, K 2 HPO 4 0.5g / L, MgSO 4 0.5g / L, NaCl 0.2g / L, but Bradyrhizobium grows slowly in the above medium, which not only increases the ferm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/41
CPCC12N1/20
Inventor 高同国朱宝成侯慧云郭晓军
Owner HEBEI AGRICULTURAL UNIV.
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