Composite agarose microsphere and preparation method and application thereof
A technology of agarose microspheres and agarose, which is applied in the field of materials, can solve the problems of short drug action time, large toxic and side effects in the human body, poor biological stability, etc., and achieve the effect of easy promotion and application, cheap and large coating volume
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[0034] Example 1:
[0035] A composite agarose microsphere of the present invention, comprising hydroxyurea, β-D-glucose oxidoreductase and horseradish peroxidase, hydroxyurea, β-D-glucose oxidoreductase and horseradish peroxidase package The compound agarose microspheres are formed in the agarose. figure 1 It is a schematic diagram of agarose microspheres coated with Cascade enzyme. The preparation method includes the following steps:
[0036] (1) Prepare the organic phase: mix 18 mL of toluene, 7 mL of chloroform and 0.3 mL of sorbitan fatty acid ester to form the organic phase. Add the above organic phase to a 100 mL flask and preheat to 55°C at 1000 rpm.
[0037] (2) Prepare the water phase: Take 5 ml of water and add 84 mg agarose, heat to dissolve, and get an agarose solution. When the temperature of the agarose solution drops to 55°C, add 0.1 mL of β-D-glucose oxidoreductase solution with a pH of 7.0 and a concentration of 1 mg / mL and 0.5 mL of agarose with a pH of 7.0 and a...
Example Embodiment
[0039] Example 2:
[0040] A composite agarose microsphere of the present invention, comprising hydroxyurea, β-D-glucose oxidoreductase and iron tetroxide nanoparticles, hydroxyurea, β-D-glucose oxidoreductase and iron tetroxide nanoparticles package The compound agarose microspheres are formed in the agarose. The preparation method includes the following steps:
[0041] (1) Prepare the organic phase: mix 18 mL of toluene, 7 mL of chloroform and 0.3 mL of sorbitan fatty acid ester to form the organic phase. Add the above organic phase to a 100 mL flask and preheat to 55°C at 1000 rpm.
[0042] (2) Prepare the water phase: Take 5 ml of water and add 84 mg agarose, heat to dissolve, and get an agarose solution. When the temperature of the agarose solution dropped to 55°C, 0.1 mL of β-D-glucose oxidoreductase solution with pH 7.0 and concentration of 1 mg / mL and 0.5 mL of pH 7.0 and concentration of 1 mg / mL were added. The ferroferric oxide nanoparticle solution is ultrasonically di...
Example Embodiment
[0044] Experimental example 1:
[0045] Take 200 μL of the agarose composite microspheres of Example 1 (the total gel mass is 0.3 mg, and the number of microspheres is 2.0×10 5 ) Add a glucose solution with a concentration of 10 mmol / L, react for 30 min, add Griess reagent (nitrogen monoxide detection reagent), put it into an ultraviolet spectrophotometer to detect the characteristic absorption peak, and use the unsupported nitric oxide precursor Enzyme-coated agarose microspheres served as a blank control.
[0046] figure 2 This is the UV spectrum of the agarose composite microspheres. It can be seen from the figure that the agarose composite microspheres produce nitric oxide, which makes the Griess reagent have a characteristic absorption peak at 540nm.
[0047] image 3 It is the curve of nitric oxide production and time. It can be seen from the figure that as time goes by, the content of nitric oxide shows an S curve growth.
[0048] From figure 2 with image 3 It is known that...
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