A kind of Leuconostoc enterococcus and its application in fermented sauerkraut
A technology for Leuconostoc enterica and sauerkraut, which is applied in the directions of Leuconostoc, application, bacteria, etc., can solve the problems of sauerkraut with a mellow and rich flavor, unstable product quality, perishable and stale and other problems, and achieves inhibition of miscellaneous bacteria and harmful bacteria. The effect of increasing the growth, reducing the hidden danger of eating, and increasing the yield of vegetables
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Embodiment 1
[0024] The screening and identification process of embodiment 1 bacterial classification
[0025] Strain isolation and purification
[0026] A small amount of traditional fermented sauerkraut made by many citizens in Harbin was selected, and the surface was washed with sterile water and 70% ethanol three times alternately, then rinsed with sterile water, and the excess water was drained. Get sauerkraut leaf and grind out juice, carry out gradient dilution with sterile water (10 -1 、10 -2 、10 -3 、10 -4 、10 -5 ), take 50 microliters of the culture solution of each dilution concentration and spread it on the MRS solid medium and culture it at a constant temperature of 37 ° C for 48 hours, pick a single colony with a large calcium dissolution circle and streak it on the MRS solid medium for purification. Eleven colonies producing calcium-dissolving circles were selected and named DN-LP-X1, DN-LP-X2, DN-LP-M1, DN-LP-M2, DN-LP-W1, DN-LP-H2, DN -LA-H7, DN-LM-Y2, DN-LM-Y3, DN-LM...
Embodiment 2
[0037] Example 2 Application of Leuconostoc mesenteroides DN-LM-D2 bacteria in pickled sauerkraut
[0038] Pick the Leuconostoc mesenteroides DN-LM-D2 bacterial solution stored in the glycerol tube and streak it on the MRS plate, culture at 37°C for 48-72h, pick a single colony and inoculate it into 20-30ml MRS liquid medium, shake at 37°C at 150r Bed culture 18-, as seed liquid, to be expanded culture. The formula of the expanded fermentation medium is peptone 10g / L, yeast powder 5g / L, beef extract 5g / L, tomato juice 100ml / L, and 1M NaOH is used to adjust the pH of the medium to 6.2-6.4, and the intestines are obtained after 18 hours of cultivation. Leuconostoc membranes. The bacterial liquid is used to ferment sauerkraut:
[0039] 1) Choose fresh Chinese cabbage, peel off the outer leaves (rotted or dehydrated leaves), and place in a cool, dry and ventilated place for 2-3 days.
[0040] 2) Cleaning: Wash the Chinese cabbage with clean water, drain the water, and place it ...
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