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Antibacterial composition, preparation method thereof, and application of antibacterial composition to mouthwash

An antibacterial composition and antimicrobial peptide technology, applied in the field of daily chemicals, can solve problems such as oral mucosal damage, oral flora imbalance, dry mouth, etc., reduce gingival inflammation and bleeding, slow down the formation of dental calculus, and improve the use of taste effect

Inactive Publication Date: 2019-07-16
王飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It can be seen that at present, chemical raw materials are generally used in the raw materials of mouthwash at home and abroad, which contain many ingredients that the human body cannot swallow. The side effects are large. Repeated use for a long time will also kill the beneficial bacteria in the mouth, and the bacteria in the mouth are prone to appear. Imbalance, leading to oral mucosal damage, causing many oral diseases and other diseases, children are not allowed to use
In addition, there are also some mouthwashes with natural plant essential oils as the main active ingredient on the market, but in order to improve product stability, a large amount of ethanol is generally added. Products can easily cause dry mouth, destroy the mucus protective layer that keeps the mouth moist, and cause a tingling sensation

Method used

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  • Antibacterial composition, preparation method thereof, and application of antibacterial composition to mouthwash
  • Antibacterial composition, preparation method thereof, and application of antibacterial composition to mouthwash
  • Antibacterial composition, preparation method thereof, and application of antibacterial composition to mouthwash

Examples

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Embodiment 1

[0027] This embodiment provides an antibacterial composition, the raw material components include: 0.001% antibacterial peptide, 0.1% glycerin, and the balance is water;

[0028] The antimicrobial peptide is prepared with reference to the method described in Example 1 of the Chinese invention patent CN103468673B specification, and the specific preparation method is as follows:

[0029] 1. Construction of pET28a-GBB vector

[0030] According to the literature Lee EK et al.Peptides.2011, 32(6):1123-30. The amino acid sequence of the antimicrobial peptide GBB disclosed: GRFKRFRKKFKKLFKKLS* (SEQ ID NO.1), the gene sequence P1 of the antimicrobial peptide GBB was translated and designed, using The software Genetyx designed the complementary sequence P2 of P1, using the annealed products of P1 and P2 as templates, and P3 and P4 as primers, by PCR (PCR conditions: 98°C for 1min; 98°C for 15s, 63°C for 15s, 72°C for 15s, 35cycles; 72°C for 5 min) to obtain the GBB gene, using Phusion...

Embodiment 2

[0049] This embodiment provides an antibacterial composition, the raw material components include: 0.05% antibacterial peptide, 10% glycerin, and the balance is water, wherein the preparation method of the antibacterial peptide is the same as that in Example 1.

[0050] The preparation method of described antibacterial composition, comprises the steps:

[0051] (1) Add glycerin to water, then heat to 100°C, keep warm for 20min, then cool down to 25°C to obtain a mixed solution;

[0052] (2) Add antimicrobial peptides to the mixed solution, stir for 20min at a stirring speed of 2000r / min, and vacuum degassing to obtain a pH of 7.2 and a density of 1.010g / cm 3 antibacterial composition.

Embodiment 3

[0054] This embodiment provides an antibacterial composition, the raw material components include: 0.02% antibacterial peptide, 1% glycerin, 1% apple juice, and the balance is water, wherein the preparation method of the antibacterial peptide is the same as that in Example 1.

[0055] The preparation method of described antibacterial composition, comprises the steps:

[0056] (1) Add glycerin to water, then heat to 100°C, keep warm for 20min, then cool down to 25°C to obtain a mixed solution;

[0057] (2) Add apple juice and antimicrobial peptides successively to the mixed solution, stir for 25min at a stirring speed of 1000r / min, and vacuum degassing to obtain a pH of 5.8 and a density of 0.913g / cm 3 antibacterial composition.

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Abstract

The invention relates to an antibacterial composition, a preparation method thereof, and an application of the antibacterial composition to mouthwash. The antibacterial composition comprises the following raw material components of antibacterial peptide, glycerine and water, wherein the mass of the antibacterial peptide is 0.001-0.05wt% of the total mass of the antibacterial composition, and the mass of the glycerine is 0.1-10wt% of the total mass of the antibacterial composition. The antibacterial composition disclosed by the invention can effectively restrain buccal inflammation pathogenic bacteria of albicans, streptococcus mutans and porphyromonas gingivalis, dental plaque can be effectively reduced, gum inflammation and bleeding can be alleviated, and the formation of calculus is slowed, so that the antibacterial composition can be used for preventing and controlling various buccal inflammations. The antibacterial composition disclosed by the invention does not contain any chemical additive ingredients, and is safe and free from toxic and side effects; and through a reasonable prescription, the eating moth feel of the product namely the antibacterial composition is notably improved, and the antibacterial composition has higher acceptance degree than products sold in the market, and is suitable for promotion and application.

Description

technical field [0001] The invention belongs to the technical field of daily chemicals, and in particular relates to an antibacterial composition, its preparation method and its application in mouthwash. Background technique [0002] Mouthwashes are generally divided into two types: prescription and over-the-counter. Prescription mouthwash contains more antibacterial ingredients, usually chlorhexidine, which can effectively inhibit the growth of dental plaque, prevent and control periodontal tissue inflammation, but long-term use is more likely to cause tooth surface staining And some changes in taste, so that taste buds reduce taste, and inhibit the secretion of saliva, resulting in dry mouth, burning pain and other symptoms. There are some prescription mouthwashes, such as "Koutai", which also contain antibiotic ingredients such as metronidazole. This kind of mouthwash needs to be used strictly according to the doctor's advice, and it is not suitable for the general popul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/34A61K8/9789A61K8/9794A61P1/02A61P31/04A61P31/10A61Q11/00
CPCA61K8/345A61K8/64A61K8/9789A61K8/9794A61P1/02A61P31/04A61P31/10A61Q11/00
Inventor 王飞
Owner 王飞
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