Unlock instant, AI-driven research and patent intelligence for your innovation.

Apple fruit 'ester type' character identification SNP (single nucleotide polymorphism) molecular marker, primer and application of marker

A technology of molecular markers and apples, which is applied in the field of crop genetics and breeding, can solve problems such as no relevant records, achieve the effects of improving efficiency, improving breeding work efficiency, and realizing early selection

Active Publication Date: 2019-07-26
SHANDONG INST OF POMOLOGY
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant record about the relevant molecular markers of apple fruit ester aroma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apple fruit 'ester type' character identification SNP (single nucleotide polymorphism) molecular marker, primer and application of marker
  • Apple fruit 'ester type' character identification SNP (single nucleotide polymorphism) molecular marker, primer and application of marker
  • Apple fruit 'ester type' character identification SNP (single nucleotide polymorphism) molecular marker, primer and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The SNP molecular markers identified by the apple fruit ester volatile components of the present invention are obtained by the following methods:

[0036] 1. Reagents and instruments used

[0037] Genomic DNA (gDNA) of individuals in the test population was extracted using the Plant Genomic DNA Kit (TIANGEN) kit, which was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; restriction enzymes for enzyme digestion EcoRI Purchased from Sigma-Aldrich Company in the United States; reagents required for post-processing of enzyme-digested samples: adapters, ATP, ligase, blunt-end enzyme, etc. were purchased from Illumina Company in the United States; The IlluminaHiseq 2500 sequencing platform (USA) was used.

[0038] 2. SNP molecular marker acquisition method

[0039] (1) Construction of genetic linkage map of 'Red Star' X 'Guoguang'

[0040] The "ester-flavored" apple variety 'Red Star' with higher fruit volatile components was used as the female parent, ...

Embodiment 2

[0061] In order to efficiently, quickly and accurately detect the SNP molecular marker of the present invention, it can be made into a SNP gene chip. The above-mentioned PCR primers for amplifying the SNP molecular markers are fixed on the SNP gene chip, and the gene chip is manufactured by Affymetrix Company of the United States. The genomic DNA of the test population was extracted, KASP reaction was performed on the SNP gene chip, the SNP molecular marker was amplified, and the content of volatile esters in the fruit of the test population was quickly identified through the genotype of the SNP molecular marker. The specific operation is as follows:

[0062] The young leaves of 88 'Hongxing'×'Guoguang' mapping population strains were collected, and the genomic DNA of the experimental population and varieties were extracted using the Plant Genomic DNA Extraction Kit (Plant Genomic DNA Kit, Beijing Tiangen Company). KASP reaction was performed on the SNP gene chip. KASP ampli...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an apple fruit 'ester type' character identification SNP (single nucleotide polymorphism) molecular marker, a primer and an application of the marker. A molecule is marked foran A-G conversion type. An amplification primer and an SNP gene chip are developed, and early identification of apple fruit ester volatility component characters is achieved. The content of apple fruit ester volatility components can be rapidly and accurately identified by the SNP molecular marker and the SNP gene chip, apple hybrid seedling breeding time is shortened, and economic costs are reduced. Marker sources are provided for assisted breeding of apple flavor quality molecular markers bases are provided for high-quality new germplasms bred by genetic engineering approaches, and the molecular marker has important theoretical significance and practical application values.

Description

technical field [0001] The present invention relates to a SNP (single nucleotide polymorphism) molecular marker and its primers for the identification of "ester flavor" traits of apple fruit, and also relates to a SNP chip and a method for quickly identifying the "ester flavor" of apple fruit. The invention belongs to the technical field of crop genetic breeding. Background technique [0002] Quality breeding is the main theme of apple breeding in the world, and the primary goal of new variety breeding is to have excellent fruit quality. The composition of fruit quality mainly includes the appearance of fruit surface, fruit flavor, pulp texture and nutritional components. The direct factor of purchasing fruit. The components that determine the aroma and smell of ripe apple fruit are very complex, including about 350 volatile components, and the aroma components in these components are mainly low-molecular esters, such as acetate, propionate, butyrate, caproate , butanol e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 王海波常源升何平王森何晓文王传增孙家正李慧峰李林光
Owner SHANDONG INST OF POMOLOGY