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Detection method of vitamin K2 in multiple-vitamin-mineral compound preparation

A compound preparation and detection method technology, applied in the field of analytical chemistry, can solve problems such as development and quality control obstacles, complex mobile phase, unsuitable detection, etc., and achieve scientific and effective results in product development and quality control assurance and determination

Active Publication Date: 2019-07-26
HANGZHOU MINSHENG HEALTHCARE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the method mentioned in the above-mentioned patent is just a simple compound preparation of vitamin K 2 It is not suitable for the detection of complex formulas, especially vitamin K in multi-vitamin and mineral compound preparations 2 detection; the pretreatment is just ordinary constant volume, constant temperature water bath oscillation treatment, for samples with high content of insoluble components such as calcium, it is difficult to obtain vitamin K 2 The extraction is complete and the accurate quantification of the extract is guaranteed; in addition, the mobile phase is more complicated
[0007] In addition, there is an invention patent of Jinan Yingsheng Biotechnology Co., Ltd. "A method for simultaneously detecting multiple fat-soluble vitamins in blood samples" (application number 201510581956.X), which is only applicable to the detection of fat-soluble vitamins in blood samples. Not suitable for vitamin K in multivitamin mineral compositions 2 detection
[0008] In summary, there is currently no vitamin K suitable for use in multivitamin mineral compositions. 2 The national standard detection method, the published relevant patents, and the methods provided in the papers all have certain defects, and cannot really meet the requirements of vitamin K in multivitamin and mineral compositions (especially compositions with high insoluble components such as calcium and magnesium). 2 This has brought obstacles and inconvenience to the development and quality control of vitamin mineral functional foods and drugs

Method used

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  • Detection method of vitamin K2 in multiple-vitamin-mineral compound preparation
  • Detection method of vitamin K2 in multiple-vitamin-mineral compound preparation
  • Detection method of vitamin K2 in multiple-vitamin-mineral compound preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Take 50 tablets (1g / tablet) of multi-vitamin and mineral compound preparations, grind them to 40 mesh with a traditional Chinese medicine grinder, and use them as samples.

[0038] (2) Accurately weigh 25g of the sample into a 200mL brown volumetric flask, accurately measure 40mL of hydrochloric acid-isopropanol with an HCl concentration of 1mol / L, shake well (do not need to constant volume), place in frequency 35Hz, power 0.5W Under the conditions of ultrasonic oscillation for 10min. After the sonication, centrifuge at room temperature and 5000r / min for 10min, and take the supernatant. The supernatant was filtered with a 0.45 μm filter membrane, and the filtrate was used to determine vitamin K in the sample by high performance liquid chromatography 2 (Menadione) relative peak area A, calculate vitamin K in sample according to formula (1) 2 content c, as vitamin K 2 The retention time is qualitative, the external standard method of peak area is quantitative, the ...

Embodiment 2

[0052] (1) Take 50 tablets (1g / tablet) of multi-vitamin and mineral compound preparations, grind them to 60 mesh with a traditional Chinese medicine grinder, and use them as samples.

[0053] (2) Accurately weigh 25g of the sample into a 200mL brown volumetric flask, accurately measure 50mL of hydrochloric acid-isopropanol with an HCl concentration of 3mol / L, shake well (no constant volume required), and place in a frequency 45Hz, power 1W Under the condition of ultrasonic oscillation for 20min. After the ultrasound is over, centrifuge for 10 minutes at room temperature and 5000r / min, take the supernatant and filter it with a 0.45μm filter membrane, and use high-performance liquid chromatography to determine vitamin K in the sample. 2 (Menadione) relative peak area, adopt embodiment 1 formula (1) to calculate vitamin K 2 The content c is qualitative by retention time, and quantified by the external standard method of peak area, and the conditions are: C 18 ODS column (150*4....

Embodiment 3

[0059] (1) Take 50 tablets (1g / tablet) of multi-vitamin and mineral compound preparations, grind them to 80 mesh with a traditional Chinese medicine grinder, and use them as samples.

[0060] (2) Accurately weigh 25g of the sample into a 200mL brown volumetric flask, accurately measure 60mL of hydrochloric acid-isopropanol with an HCl concentration of 5mol / L, shake well (do not need to constant volume), place in frequency 50Hz, power 1.5W Under the condition of ultrasonic oscillation for 30min. After ultrasonication, centrifuge at room temperature and 6000r / min for 30min, take the supernatant and filter it with a 0.45μm filter membrane, and use high-performance liquid chromatography to determine vitamin K in the sample. 2 (Menadione) relative peak area, adopt embodiment 1 formula (1) to calculate vitamin K 2 The content c is qualitative by retention time, and quantified by the external standard method of peak area, and the conditions are: C 18 ODS column (150*4.6, 5 μm), the...

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Abstract

The invention particularly relates to a detection method of vitamin K2 in a multiple-vitamin-mineral compound preparation and application of the detection method. The method comprises the steps that firstly the vitamin K2 in the multiple-vitamin-mineral compound preparation is ultrasonically extracted by an organic solvent, and then liquid phase detection is carried out. The detection method has the characteristics of high accuracy, good repeatability and easy operation. A national standard test method applied to the detection of the vitamin K2 in the multiple-vitamin-mineral compound preparation does not exist currently, methods provided by published relevant patents and documents have certain defects and cannot actually meet the detection requirements of the vitamin K2 of the multivitamin mineral composition multiple-vitamin-mineral compound preparation, so that obstacles and inconvenience are brought for the development and quality control of vitamin-mineral functional food and medicine. Based on the reasons, the detection method for the vitamin K2 in the multiple-vitamin-mineral compound preparation is provided, a gap in the detection of the vitamin K2 in the multiple-vitamin-mineral compound preparation is filled, and a guarantee is provided for the development and quality control of the vitamin-mineral functional food and medicine.

Description

[0001] (1) Technical field [0002] The invention relates to vitamin K in a multivitamin mineral composition 2 The detection method belongs to the field of analytical chemistry. [0003] (2) Background technology [0004] Vitamin K 2 It is a fat-soluble vitamin, a derivative of the naphthoquinone group with the biological activity of phylloquinone. It is one of the indispensable important vitamins in the human body. It is the only biologically active form of vitamin K. It is mostly used to accelerate blood coagulation and maintain blood coagulation. Time, treatment of bleeding caused by vitamin K deficiency. [0005] Vitamin K 2 It is unstable when exposed to light and alkali, easy to decompose, but resistant to acid and heat. According to this characteristic, the current vitamin K 2 All are processed by microcapsule embedding technology to increase its stability. The embedded shell is a polymer material, and vitamin K 2 It is a fat-soluble vitamin, so when testing, firs...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨善岩钟建荣李睿杰黄凤琴刘丽云陈稳竹
Owner HANGZHOU MINSHENG HEALTHCARE CO LTD
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