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Preparation method of fluorescent/photoacoustic/SPECT multi-mode imaging nano probe and application of probe in cancer diagnosis

A multi-mode imaging and nano-probe technology, which is applied in the field of nano-biomedical materials, can solve the problems of increasing the toxicity and side effects of normal tissues, low spatial resolution, and reducing imaging quality, etc., to achieve improved cancer diagnosis efficiency, high sensitivity, and high cancer The effect of diagnostic efficiency

Active Publication Date: 2019-07-30
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is characterized by strong tissue penetration of ray signals, but low spatial resolution
125 I is a commonly used SPECT imaging probe, but it is easy to accumulate in the thyroid gland during the systemic circulation, and the amount reaching the cancer site is small, which reduces the imaging quality and increases the toxic and side effects on normal tissues

Method used

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  • Preparation method of fluorescent/photoacoustic/SPECT multi-mode imaging nano probe and application of probe in cancer diagnosis
  • Preparation method of fluorescent/photoacoustic/SPECT multi-mode imaging nano probe and application of probe in cancer diagnosis
  • Preparation method of fluorescent/photoacoustic/SPECT multi-mode imaging nano probe and application of probe in cancer diagnosis

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Experimental program
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Embodiment 1

[0038] A preparation method of fluorescence / photoacoustic / SPECT multimode imaging nanoprobe, comprising the steps of: 9.0mgPEG-PTyr( 125 1)-ICG was dissolved in 1mL DMSO to obtain a polymer solution, and then the polymer solution was slowly added dropwise to 10mL phosphate buffered saline (PBS, 1Mm, pH=7.2) under electromagnetic stirring, and then the solution was placed in Stir at room temperature for 14 hours to stabilize the micelles, and then use a dialysis bag with a molecular weight cut-off of 1000 for 50 hours to completely remove the DMSO solvent in the system to obtain PEG-PTyr( 125 I)-ICG polymer micelles, which are the final multi-mode imaging nanoprobes.

[0039] Wherein, the PEG-PTyr ( 125 1)-ICG preparation method, comprises the steps:

[0040] 1) Weigh 0.6mmol PEG 2000 -NH 2 Put 4.8mmol Tyr-NCA in a 50mL round bottom flask, add 11mL dry DMF, and react under closed stirring at 40°C for 22 hours to obtain a reaction solution;

[0041] 2) Slowly add the reacti...

Embodiment 2

[0047] A preparation method of fluorescence / photoacoustic / SPECT multimode imaging nanoprobe, comprising the steps of: 11.0mgPEG-PTyr( 125 1)-ICG was dissolved in 1mL DMSO to obtain a polymer solution, and then the polymer solution was slowly added dropwise to 8mL phosphate buffer saline (PBS, 1Mm, pH=7.6) under electromagnetic stirring, and then the solution was placed in Stir at room temperature for 10 hours to stabilize the micelles, and then use a dialysis bag with a molecular weight cut-off of 1000 for 46 hours to completely remove the DMSO solvent in the system to obtain PEG-PTyr( 125 I)-ICG polymer micelles, which are the final multi-mode imaging nanoprobes.

[0048] Wherein, the PEG-PTyr ( 125 1)-ICG preparation method, comprises the steps:

[0049] 1) Weigh 0.4mmol PEG 2000 -NH 2 Put 3.2mmol Tyr-NCA in a 50mL round bottom flask, add 8mL dry DMF, and react under closed stirring at 40°C for 26 hours to obtain a reaction solution;

[0050] 2) Slowly add the reaction ...

Embodiment 3

[0056] A preparation method of fluorescence / photoacoustic / SPECT multimode imaging nanoprobe, comprising the following steps: 10.0mgPEG-PTyr( 125 1)-ICG was dissolved in 1mL DMSO to obtain a polymer solution, and then the polymer solution was slowly added dropwise to 9mL phosphate buffered saline (PBS, 1Mm, pH=7.4) under electromagnetic stirring, and then the solution was placed in Stir at room temperature for 12 hours to stabilize the micelles, and then use a dialysis bag with a molecular weight cut-off of 1000 for 48 hours to completely remove the DMSO solvent in the system to obtain PEG-PTyr( 125 I)-ICG polymer micelles, which are the final multi-mode imaging nanoprobes.

[0057] Wherein, the PEG-PTyr ( 125 1)-ICG preparation method, comprises the steps:

[0058] 1) Weigh 0.5mmol PEG 2000 -NH 2 Put 4mmol Tyr-NCA in a 50mL round bottom flask, add 10mL dry DMF, and react under closed stirring at 40°C for 24 hours to obtain a reaction solution;

[0059] 2) Slowly add the r...

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Abstract

The invention discloses a preparation method of a fluorescent / photoacoustic / SPECT multi-mode imaging nano probe and application of the probe in cancer diagnosis. The preparation method comprises the following steps that PEG-PTyr(125I)-ICG is dissolved in DMSO to obtain a polymer solution; the polymer solution is slowly added into a phosphate buffer solution under electromagnetic stirring and stirred for 10-14 hours at room temperature; dialysis and purification are carried out so as to obtain PEG-PTyr(125I)-ICG polymer micelle, namely the multi-mode imaging nano probe. ICG capable of carryingout fluorescent / photoacoustic imaging and 125I capable of carrying out SPECT imaging are fused to the same polymer, and the fluorescent / photoacoustic / SPECT multi-mode imaging nano probe with high sensitivity, high spatial resolution and low background signals at the same time is prepared. Comprehensive medical images can be obtained. Compared with an existing single-mode imaging probe, the methodcan improve the accuracy of cancer diagnosis.

Description

technical field [0001] The invention belongs to the field of nano biomedical materials, and relates to a preparation method of a fluorescence / photoacoustic / SPECT multi-mode imaging nanoprobe and its application in cancer diagnosis. Background technique [0002] According to the statistics of the World Health Organization, cancer has become the number one killer of human beings. Although the drugs and means of treating cancer have made great progress, the five-year survival rate of cancer patients has not been significantly improved compared with 20 years ago, and the most effective means to improve the cure rate of cancer is still "early detection, early treatment". Therefore, the development of diagnostic methods capable of early, rapid detection and accurate localization of cancer can greatly reduce the mortality of cancer patients. [0003] Molecular imaging technology can visualize the biological process in the living state and obtain intuitive medical images. It has b...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/22A61K51/06A61K51/08A61K51/12A61K101/02
CPCA61K49/0002A61K49/0034A61K49/0054A61K49/0082A61K49/227A61K51/06A61K51/08A61K51/1227
Inventor 刘鉴峰杨丽军黄帆刘金剑张玉民任春华杨翠红张从柔
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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