Dewaxed garlic volatile essential oil and extraction method and application thereof

An extraction method, technology of garlic, applied in application, essential oil/fragrance, botanical equipment and method, etc.

Inactive Publication Date: 2019-08-06
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the control of crop soil-borne diseases caused by Phytophthora by biological fumigation

Method used

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  • Dewaxed garlic volatile essential oil and extraction method and application thereof
  • Dewaxed garlic volatile essential oil and extraction method and application thereof
  • Dewaxed garlic volatile essential oil and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation and chemical composition analysis of dewaxed garlic volatile essential oil

[0035] 1 Materials and methods

[0036] 1.1 Test material

[0037] Garlic is peeled, rinsed with cold water, freeze-dried, crushed and passed through an 80-mesh sieve to obtain garlic powder, which is stored in a ziplock bag at low temperature (4°C) and protected from light.

[0038] 1.2 Instruments and equipment

[0039] Gas chromatography-mass spectrometry (Shimadzu Corporation of Japan); rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); supercritical fluid extraction device (Waters, USA); SW-CJ-1FD clean bench (Sujing Antai Technology Co., Ltd. Ltd.).

[0040] 1.3 Method

[0041] 1.3.1 Essential oil extraction method

[0042] Supercritical fluid extraction method: get 70g freeze-dried garlic samples, put into supercritical extraction device; open water bath, make extraction temperature reach 35 ℃, extraction pressure 200bar (20MPa), CO Flow velocity 20g / min...

Embodiment 2

[0047] Identification of chemical constituents of volatile essential oil of dewaxed garlic:

[0048] 1. Gas chromatography analysis (GC-MS)

[0049] The essential oil was diluted to 100g / L with n-hexanol, and 1 μL was injected into Agilent7890-5975GC-MS, using 30×0.25mmi.d, 0.25μm, DB-5MS fused silica capillary column (Agilent Technologies). The inlet temperature was 250°C, helium was used as the carrier gas, and the flow rate was 1.0 mL / min. The initial oven temperature was held at 45°C for 2 minutes, raised to 200°C at 5°C / min, held for 2 minutes, raised to 320°C at 15°C / min, held for 5 minutes. The ionization mode is 70eV electron impact. The full-scan monitoring mode is adopted, the mass scanning range is 35-500m / z, and the mass spectrum is obtained, and the accelerating voltage is turned on after a solvent delay of 5 minutes.

[0050] 2. Headspace Solid Phase Microextraction (HS-SPME) of Volatile Compounds

[0051] Volatile compounds were absorbed from EOD using sandw...

Embodiment 3

[0059] Inhibition test of dewaxed garlic volatile essential oil and its main components against tobacco black shank

[0060] 1.1 Inhibitory test of dewaxed garlic volatile essential oil on tobacco black shank

[0061] Fumigation test: prepare an oat medium plate, connect the black shank bacterial cake in the center of the medium, turn the petri dish upside down, add the volatile essential oil of dewaxed garlic to the lid of the petri dish, and the final concentration of the essential oil in the dish lid is 1600, 1400, 1200 , 1000, 800, 0μL / L, observe and record the colony diameter after 4 days, and calculate Ec50.

[0062] 1.2 Inhibitory test of diallyl disulfide (85%) on tobacco black shank bacteria

[0063] Fumigation test: Prepare an oat culture medium plate (glass dish), connect the black shank bacteria cake in the center of the medium, turn the culture dish upside down, and drop 8, 7, 6, 5, 4, 3, 2, 1 μL into the lid of the culture dish respectively Diallyl disulfide (8...

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Abstract

The invention provides dewaxed garlic volatile essential oil and an extraction method and application thereof, and relates to the technical field of volatile oil application. The extraction method takes garlic as a raw material to obtain a mixture containing essential oil through one-step extraction by a supercritical fluid extraction method, and performing elution, dehydration and ethanol recovery to obtain the dewaxed garlic volatile essential oil. The obtained dewaxed garlic volatile essential oil has a better inhibiting effect on tobacco black shank, the main active ingredient of the dewaxed garlic volatile essential oil is diallyl disulfide, and the dewaxed garlic volatile essential oil has a stronger fumigation effect on the tobacco black shank. Pot experiments show the diallyl disulfide treatment could significantly reduce the incidence of the tobacco black shank and the number of the tobacco black shank in rhizosphere soil. In addition, the compound use of carvacrol and the diallyl disulfide can significantly improve the control effect on the tobacco black shank, reduce the number of black shank bacteria in the soil, and also relieve garlic odor to some extent.

Description

technical field [0001] The invention belongs to the technical field of volatile oil application, and in particular relates to a dewaxed garlic volatile essential oil and its extraction method and application. Background technique [0002] Phytophthora oomycetes are important plant pathogens, infecting almost all dicotyledonous plants and some grasses, and according to statistics, cause global economic losses of nearly 50 billion US dollars every year. Due to the wide range of hosts and multiple transmission routes, and the fact that pathogenic bacteria can form oospores and other dormant bodies to survive in the soil for a long time, so far, the prevention and control of Phytophthora is still a worldwide problem. Soil fumigation is one of the most effective and direct methods to control Phytophthora capsici. In the past few decades, methyl bromide has been widely used as a high-efficiency soil fumigant in the prevention and control of soil-borne diseases, but it has serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A01N65/42A01P3/00
CPCC11B9/025C11B9/022A01N65/00A01N65/42
Inventor 张成省芶剑渝荆常亮刘明宏赵栋霖韩小斌李义强赵建徐宗昌彭玉龙邹平刘京袁源
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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