High-flux medicine automatic screening device and method

An automatic screening and high-throughput technology, applied in biochemical equipment and methods, measuring devices, biochemical cleaning devices, etc., can solve the problems of expensive production costs, reduce experimental costs, expand utilization, and improve screening efficiency Effect

Inactive Publication Date: 2019-08-13
苏州永沁泉智能设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Existing microfluidic chips usually use glass, plexiglass, silicone and other materials, which are relatively expensive to manufacture and require sophisticated pumps to drive reagents to flow in the channel for reaction.

Method used

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  • High-flux medicine automatic screening device and method
  • High-flux medicine automatic screening device and method
  • High-flux medicine automatic screening device and method

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Embodiment 2

[0047] Taking the drug screening of breast cancer cells as an example, the specific method of using the device of the present invention is explained in detail, figure 1 Schematic diagram of dropping the hydrogel solution with breast cancer cells into the well plate.

[0048] The preparation method of the hydrogel solution with breast cancer cells is as follows: the cells in the logarithmic growth phase were washed with 5 mL of PBS to remove the metabolites in the cell growth phase, and then 3 mL of trypsin with a concentration of 0.25% was added to the React at 37 degrees for three minutes, let the adherent cells be in a free state, add 3mL of medium trypsin to stop the digestion of the cells, put them in a centrifuge tube and centrifuge for a few minutes, let the cells sink in the centrifuge tube Below, take out the centrifuge tube, pour off the supernatant, add the medium mixed with hydrogel, and then use the counting plate to count the number of cells, so that the hydrogel ...

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Abstract

The invention provides a high-flux medicine automatic screening device and method. The automatic screening device comprises a three-dimensional moving device, an air channel system and an electrostatic field on-off control system; the three-dimensional moving device comprises four motor modules arranged at X, Y, and Z axial directions, a sprayer head and a baseplate platform ; the baseplate platform is arranged on an upper surface of an Y-axle motor module in a sliding manner; the sprayer head is arranged at a front end surface of the X-axle motor module and dead against the baseplate platform, and the three-dimensional moving device is controlled through a displacement control system; and the displacement system, the air channel system and the electrostatic field on-off control system arecontrolled through an upper computer control system. The device realizes the automatic control, the working intensity is reduced, the operation error is reduced, and the screening efficiency is improved; the technology for manufacturing micro-droplet through high-voltage electric field reduces the medicament dosage, and the experimental cost is greatly reduced, thereby satisfying a demand of quick, high-efficient and microscale medicine screening technology.

Description

technical field [0001] The invention relates to the field of biomechanics, in particular to a high-throughput drug automatic screening device and method. Background technique [0002] With the emergence of combinatorial chemistry, drug screeners are faced with more and more new targets or potential active ingredients, under which high-throughput screening technology came into being. High-throughput screening technology Since the 1990s, high-throughput screening based on 96-well plates has emerged due to the development of science and technology. Due to its fast and efficient characteristics, it is suitable for preliminary screening. Based on the advanced experimental method, the microplate form is used as the experimental tool carrier, and the automatic operating system is used to execute the experimental process. The application of this technology has greatly improved the screening speed of target molecules, active substances and drugs, and a large number of samples can be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15C12M1/36C12M1/34C12Q1/02
CPCG01N33/15G01N33/5008
Inventor 贺永陈路路高庆邵磊
Owner 苏州永沁泉智能设备有限公司
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