Unlock instant, AI-driven research and patent intelligence for your innovation.

Construction method of lung flora transplanted rodent animal model

A technology for rodents and construction methods, applied in animal husbandry and other directions, to achieve significant effects, short modeling time, and good repeatability

Inactive Publication Date: 2019-08-16
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, intestinal flora is one of the hot spots in academia, but the research on lung flora is still in the exploratory stage, and many issues need to be further studied, such as: the pathway and mechanism of lung flora affecting the body's immunity and metabolism; lung flora Screening and identification of pathogenic bacteria or pathogenic metabolites; mechanism of action of pathogenic bacteria or pathogenic metabolites and cross-linking with other factors (such as genotype); isolation of beneficial bacteria or beneficial metabolites in lung flora Identification and research on the mechanism of action, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of lung flora transplanted rodent animal model
  • Construction method of lung flora transplanted rodent animal model
  • Construction method of lung flora transplanted rodent animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) The establishment method of the flora transplantation model for lung flora research:

[0031] (1) 2-month-old C57BL / 6 mice were used as experimental animals, 15 males, weighing 30±2.0g, were randomly divided into three groups: control group, antibiotic group and transplantation group (5 each); In the environment, they were fed with basic maintenance feed; the daily food and water were sterilized, and they had free access to food and water;

[0032] (2) Preparation of antibiotics: the antibiotics used contained 0.5 mg of cefotaxime, 0.5 mg of cefuroxime, and 0.25 mg of gentamicin, mixed and dissolved in 10 mL of double-distilled water, and fully mixed;

[0033] (3) Acquisition of alveolar lavage fluid: Inject 1 mL of normal saline into the lungs of mice through the trachea using an intravenous indwelling needle, and perform repeated suction until the normal saline becomes viscous, and the alveolar lavage fluid is obtained;

[0034] Control group: mice were instilled...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a construction method of a lung flora transplanted rodent animal model and relates to the technical field of study of lung florae. According to the method, mice are taken as experiment objects, and after antibiotics are dripped to tracheae of the mice, other mouse pulmonary alveoli irrigating solutions are dripped to the tracheae of the mice, and the flora transplanted model is obtained. The method has the benefits as follows: the method is rapid, simple, feasible and remarkable in effect, has no side effects and is high in repetition rate.

Description

technical field [0001] The invention relates to the technical field of lung flora research, in particular to a method for constructing a rodent model of lung flora transplantation. Background technique [0002] Under normal physiological conditions, the human body contains as many as tens of thousands of different bacterial species, and the number of genes carried by these bacterial groups is more than 100 times that of the human body's own genes. function plays a very important role. With the deepening of research, the relationship between microorganisms and the health of the body has become increasingly clear, and they play an irreplaceable role in maintaining the normal functions of the body's immune system, circulatory system, central nervous system and digestive system. [0003] At present, intestinal flora is one of the academic hotspots, but the study of lung flora is still in the exploratory stage, and many issues need to be further studied, such as: the pathway and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 许安刘颖程雷杨振汪童丁晗
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI