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Method for evaluating reproductive toxicity of organic solvent by utilizing drosophila melanogaster

An organic solvent and reproductive toxicity technology, applied in the field of toxicity research, can solve the problems of less research on terrestrial insects, cell apoptosis, affecting gene expression, etc., and achieve the effect of accurate reproductive toxicity

Pending Publication Date: 2019-08-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, organic solvents will cause a series of negative effects on organisms, such as inducing apoptosis, affecting gene expression and even changing motor behavior.
[0003] At present, the research on toxic effects mainly focuses on plants, aquatic animals and mammals, and there are fewer studies on terrestrial insects.

Method used

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  • Method for evaluating reproductive toxicity of organic solvent by utilizing drosophila melanogaster
  • Method for evaluating reproductive toxicity of organic solvent by utilizing drosophila melanogaster
  • Method for evaluating reproductive toxicity of organic solvent by utilizing drosophila melanogaster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Reagent preparation: Dimethyl sulfoxide (DMSO), with a purity of 99.5%, was purchased from Tianjin Concord Technology Co., Ltd. and diluted with distilled water. Add equal volumes of stock solutions with different concentrations to the specified concentration in the standard culture medium, and add equal volumes of distilled water in the blank control group.

[0041] Exposure conditions: set up 2 experimental groups, 5 parallels in each group; blank control, DMSO (0.1% v / v). In each parallel experimental group, 20 pairs of newly eclovened male and female Drosophila were placed in exposure culture and placed under standard culture conditions. On the 10th day of culture, the female Drosophila was removed from the culture medium and placed on a carbon dioxide anesthesia table to separate male and female Drosophila. Medium: 77.7g / L cornmeal, 32.19g / L yeast, 10.6g / L agar, 0.726g / L CaCl 2 , 31.62g / L sucrose, 63.2g / L glucose, 2g / L potassium sorbate and 15ml / L 5% butylparaben...

Embodiment 2

[0044] Reagent preparation: Dimethyl sulfoxide (DMSO), with a purity of 99.5%, was purchased from Tianjin Concord Technology Co., Ltd. and diluted with distilled water. Add equal volumes of stock solutions with different concentrations to the specified concentration in the standard culture medium, and add equal volumes of distilled water in the blank control group.

[0045] Exposure conditions: set up 2 experimental groups, 5 parallels in each group; blank control, DMSO (0.5% v / v). In each parallel experimental group, 20 pairs of newly eclovened male and female Drosophila were placed in exposure culture and placed under standard culture conditions. On the 10th day of culture, the female Drosophila was removed from the culture medium and placed on a carbon dioxide anesthesia table to separate male and female Drosophila. Medium: 77.7g / L cornmeal, 32.19g / L yeast, 10.6g / L agar, 0.726g / L CaCl 2 , 31.62g / L sucrose, 63.2g / L glucose, 2g / L potassium sorbate and 15ml / L 5% butylparaben...

Embodiment 3

[0048] Reagent preparation: Dimethyl sulfoxide (DMSO), with a purity of 99.5%, was purchased from Tianjin Concord Technology Co., Ltd. and diluted with distilled water. Add equal volumes of stock solutions with different concentrations to the specified concentration in the standard culture medium, and add equal volumes of distilled water in the blank control group.

[0049] Exposure conditions: set up 2 experimental groups, 5 parallels in each group; blank control, DMSO (1.0% v / v). In each parallel experimental group, 20 pairs of newly eclovened male and female Drosophila were placed in exposure culture and placed under standard culture conditions. On the 10th day of culture, the female Drosophila was removed from the culture medium and placed on a carbon dioxide anesthesia table to separate male and female Drosophila. Medium: 77.7g / L cornmeal, 32.19g / L yeast, 10.6g / L agar, 0.726g / L CaCl 2 , 31.62g / L sucrose, 63.2g / L glucose, 2g / L potassium sorbate and 15ml / L 5% butylparaben...

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Abstract

The invention provides a method for evaluating reproductive toxicity of an organic solvent by utilizing drosophila melanogaster, and belongs to the technical field of toxicity research. The method comprises the following steps: when the drosophila melanogaster is placed on a solid culture medium containing the organic solvent for 10 days, observing the ovaries of female drosophila melanogaster, wherein it shows that the organic solvent has reproductive toxicity when the ovaries are abnormal. The volume concentration of the organic solvent in the solid culture medium containing the organic solvent is 0.1-1.25%. By adopting the method provided by the invention, the reproductive toxicity of the organic solvent can be accurately evaluated, and theoretical and experimental data support is provided for the dosage and concentration of the organic solvent reasonably used in agriculture.

Description

technical field [0001] The invention belongs to the technical field of toxicity research, and in particular relates to a method for evaluating the reproductive toxicity of organic solvents by using Drosophila melanogaster. Background technique [0002] Organic solvents are a class of organic compounds widely used in life and production, and can dissolve some organic compounds that are insoluble in water. Among them, the most commonly used organic solvents include styrene, trichlorethylene, ethylene glycol ether, perchlorethylene and triethanolamine, etc., which are widely used in the fields of medicine, petroleum, chemical industry, metallurgy, electronics, coatings and polymer materials. . The characteristic of organic solvents is that they are liquid at normal temperature and pressure, and have high volatility. During the dissolution process, the properties of solute and solvent do not change. However, organic solvents will cause a series of negative effects on organisms...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K10/18A23K20/163A23K20/105
CPCA01K67/033A23K10/18A23K10/30A23K20/105A23K20/163A23K50/90
Inventor 洪喻张晴路雯多郝立翀
Owner BEIJING FORESTRY UNIVERSITY
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