Time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine

Abstract

The invention provides a time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine. The reagent strip comprises a detection card and an optional fluorescence immunological quantitative analyzer, wherein the detection card comprises a sample pad, a bonding pad, a detection pad having a detection line and a quality control line, an absorption pad and a backing plate; a nano-microspheric time-resolved fluorescence-cyclosporin probe is sprayed on the bonding pad; the nano-microspheric time-resolved fluorescence-cyclosporin probe is prepared by nano fluorescent microspheres and a cyclosporine monoclonal antibody; the detection line is prepared by spraying of a cyclosporine protein derivative; the quality control line is prepared by spraying of a goat anti-mouse IgG antibody; and the cyclosporine antibody is a murine antibody. The time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine provided by the invention has high detection sensitivity, wide detection range, stable and reliable detection, simple and feasible operation, and has good commercial application prospects, and accurate and reliable measurement results can be obtained only in 5 minutes.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine. Background technique [0002] Cyclosporine A (CsA), as an immunosuppressant, has played an important role in the treatment of transplant rejection and promoted the development of organ transplantation worldwide. Although the use of CsA is often accompanied by a variety of adverse reactions, there are still hundreds of thousands of patients worldwide using CsA to suppress rejection after transplantation. [0003] Because the absorption and metabolism of CsA in different patients often show great individual differences, even with the same drug dose, the blood concentration of CsA in different patients often varies significantly; due to the liver and kidney toxicity of CsA and its narrow therapeutic dose The efficacy and toxicity of CsA are closely related to the blood conce...

AI Technical Summary

Problems solved by technology

Among them, the HPLC-MS method is accurate and sensitive for the determination of blood drug concentration, but its operation is cumbersome, the detection time is long, the detection cost is high, and expensive equipment is required. It is mainly used in the field of scientific research or as a reference method

MEIA and CMIA are commonly used detection methods at present. Their detection is highly automated and the detection results are accurate, but the reagents are expensive and need to use supporting large instruments.

The enzyme-linked immunosorbent assay has the disadvantages of long detection time, complicated operation, and poor repeatability.

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