Preparation method and application of glycosylated neohesperidin dihydrochalcone

A technology of hesperidin dihydrochalcone and glycosylation, which is applied in the field of bioengineering, can solve the problems of low solubility, long after-sweet time, slow sweet taste, etc., and achieves increased solubility, improved before-sweet and after-sweet , the effect of improving utilization efficiency

Pending Publication Date: 2019-08-23
FOSHAN GOLDEN HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in food processing, the application of NHDC still has some limitations, such as: low solubility, slow sweetness, long after-sweet time, etc.

Method used

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  • Preparation method and application of glycosylated neohesperidin dihydrochalcone
  • Preparation method and application of glycosylated neohesperidin dihydrochalcone
  • Preparation method and application of glycosylated neohesperidin dihydrochalcone

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Experimental program
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Embodiment 1

[0030] A preparation method of glycosylated neomethylhesperidin dihydrochalcone, the specific steps comprising:

[0031] Using neohesperidin dihydrochalcone as a raw material, the reaction buffer is a disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 0.9%, the pH of the buffer is 4.9, and the ratio of the raw material to the reaction buffer is 1 : 25, the reaction temperature is 55°C, and the reaction time is controlled for 6h; then in the reaction system, rhamnosidase:glucosyltransferase=15:80, the mass fraction of the total enzyme is 10% of the input neohesperidin dihydrochalcone %, add 10g / L of glucose to the system. In this reaction system, the yield of glycosylated neomethylhesperidin dihydrochalcone is 98.2%. HPLC detection results such as figure 1 , it can be seen from the figure that the reaction system can effectively glycosylate neomethylhesperidin dihydrochalcone, because the number of sugar groups linked by polyglycosylated neomethylhesper...

Embodiment 2

[0033] A preparation method of glycosylated neomethylhesperidin dihydrochalcone, the specific steps comprising:

[0034] Using neohesperidin dihydrochalcone as raw material, the reaction buffer is disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 0.1%, the pH of the reaction buffer is 2, and the ratio of the raw material to the reaction buffer is 1 : 10, the reaction temperature is 50°C, and the reaction time is controlled for 4h. Then in the reaction system, rhamnosidase:glucosyltransferase=10:90, the mass fraction of the total enzyme is 15% of the input neohesperidin dihydrochalcone %, add 5g / L glucose in the system. In this reaction system, the yield of glycosylated neomethylhesperidin dihydrochalcone is 80.1%.

Embodiment 3

[0036] A preparation method of glycosylated neomethylhesperidin dihydrochalcone, the specific steps comprising:

[0037] Using neohesperidin dihydrochalcone as a raw material, the reaction buffer is a disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 1%, the pH of the reaction buffer is 8, and the ratio of the raw material to the reaction buffer is 1 : 50, reaction temperature 60 ℃, reaction time control 6h, then in the reaction system rhamnosidase: glucosyltransferase = 90: 10, the mass fraction of total enzyme is 20% of the input neohesperidin dihydrochalcone %, add 5g / L rhamnose to the system. In this reaction system, the yield of glycosylated neomethylhesperidin dihydrochalcone is 96.8%.

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Abstract

The invention provides a preparation method of glycosylated neohesperidin dihydrochalcone. The method is characterized by comprising the specific steps that raw materials and glycosyl donors are put into a reaction buffer solution; the material and the solution are uniformly mixed; then glycosidase and glycosyltransferase are added to a reaction system, wherein the raw materials are an extract orconversion product of glycosylated neohesperidin dihydrochalcone and a derivative of the extract or conversion product. The solubility of the glycosylated neohesperidin dihydrochalcone prepared in themethod is improved, the utilization efficiency is improved, the use cost is reduced, the pre-sweetness and after-sweetness are improved, the taste is closer to that of sucrose, and therefore the application range of the glycosylated neohesperidin dihydrochalcone is wider.

Description

technical field [0001] The disclosure of the invention relates to the field of bioengineering, in particular to a preparation method and application of glycosylated neomethylhesperidin dihydrochalcone. Background technique [0002] Sweeteners are very important food additives that impart sweetness to foods. Because of their high sweetness and low calories, the market prospect is very broad. With the change of people's dietary concepts, the trend of sweeteners replacing sucrose is becoming more and more significant. Non-caloric, non-nutritive high-intensity sweeteners are the most researched field by scientists from all over the world, so they are often called functional high-intensity sweeteners. [0003] However, high-intensity sweeteners also have certain defects. For example, many products have a bitter taste and metallic taste, and the taste is impure. The bitterness can be very strong, making their applications limited. There has been considerable interest in finding...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/00A61K31/7034A61K31/716A61P3/10A61P3/08A61P3/00A23L29/30A23L33/125A23K20/163A61K8/60A61Q19/00
CPCC07H15/203C07H1/00A61P3/10A61P3/08A61P3/00A23L29/30A23L33/125A23K20/163A61K8/60A61Q19/00A23V2002/00A61K2800/10A23V2200/328
Inventor 周金林卢宇靖林丽薇黄宝华叶德晓曾超
Owner FOSHAN GOLDEN HEALTH TECH CO LTD
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