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Method for preparing ethanol by using furfural residue and oil-tea-cake as raw materials

A technology of camellia oleifera cake and furfural slag, which is applied in the field of biomass chemistry and chemical industry, to achieve the effects of reducing fermentation cost, low price and reducing use cost

Inactive Publication Date: 2019-08-27
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the direct application of camellia oleifera cake to the production of cellulosic ethanol

Method used

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  • Method for preparing ethanol by using furfural residue and oil-tea-cake as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] see process figure 1 .

[0026] 1) Furfural slag is not subjected to any detoxification treatment. It is only pulverized with a pulverizer, sieved through a 40-mesh sieve by an industrial vibrating sieve, and stored in a refrigerator at 4°C for use; After sieving through a 40-mesh sieve, put it in an oven at 50°C for 12 hours, and then put it in a desiccator for later use.

[0027] 2) Activation of yeast: Weigh 6.0 g of dry yeast and dissolve it in a sterilized 2% (w / v) glucose solution, place the mixture in a shaker at 36° C. for 15 minutes, and then shake the shaker The temperature was lowered to 34°C to continue culturing for 1h. After the activation is completed, accurately weigh 8.0 g of yeast cell culture medium, centrifuge at 4000 g for 5 min, discard the supernatant, and resuspend the centrifuged yeast cells with 1.2 mL of sterile water to prepare a 200 g / L high Concentration yeast cell seed solution.

[0028] 3) Preparation of fermentation medium: 10, 20 an...

Embodiment 2

[0031] Under the condition that the addition amount of camellia oleifera cake was 10g / L and the dosage of cellulase was 15FPU / g-cellulose, the effect of substrate concentration (10%, 15% and 20% w / w) on furfural residue and camellia oleifera cake was explored. Effects of mixed substrate fermentation processes. Other parameters of the fermentation process are as described in Example 1.

[0032] When the substrate concentration was 10% (w / w), the ethanol yield was the highest, reaching 93.39% of the theoretical value; when the substrate concentration was increased to 15% (w / w), the ethanol yield decreased slightly (89.89 %); but when the substrate concentration increased to 20% (w / w), the yield of ethanol decreased significantly (61.80%). In order to reduce the process cost of ethanol production and obtain a higher concentration of ethanol, the optimization of the subsequent fermentation process will be carried out on the basis of 15% (w / w) substrate concentration.

Embodiment 3

[0034] On the premise that the addition amount of camellia oleifera cake was 10g / L and the substrate concentration was 15% (w / w), the effect of the amount of cellulase (2.5, 5, 10, 15 FPU / g-cellulose) on the yield of ethanol was investigated. Impact. Other parameters of the fermentation process are as described in Example 1.

[0035] When the enzyme dosage was 10FPU / g-cellulose, the ethanol concentration in the solution was 30.54g / L after 120h of fermentation, and the ethanol yield reached 86.56% of the theoretical value. Compared with the fermentation system (89.89%) with an enzyme dosage of 15 FPU / g-cellulose, the fermentation efficiency was only slightly reduced.

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Abstract

The invention provides a method for preparing ethanol by using furfural residue and oil-tea-cake as raw materials. The production method provided by the invention can directly utilize tea protein in the oil-tea-cake as a nitrogen source required for the growth of yeast, and the cost of a culture media and the dosage of chemicals are reduced; meanwhile, tea saponin in the oil-tea-cake can be used as surfactants to promote the production of the ethanol. Chemical pretreatment does not exist in the whole reaction process, so that the use cost of the chemicals is reduced, and the whole technological process is environmentally friendly. Through the joint development of the furfural residue and the oil-tea-cake, further realization of the efficient use of forestry and agricultural residues is facilitated. Under the conditions that the adding amount of the oil-tea-cake is 10 g / L, the substrate concentration is 15% (w / w) and the use amount of cellulase is 10 FPU / g-cellulose, the concentration of the ethanol in a solution after fermentation for 120 h is 30.54 g / L, and the yield of the ethanol can reach 86.56% of a theoretical value.

Description

technical field [0001] The invention relates to the reuse of furfural residue and camellia oleifera cake. The furfural residue and camellia oleifera cake can produce ethanol by fermenting mixed substrates, belonging to the field of biomass chemistry and chemical industry. Background technique [0002] As an important branch of biomass resources, agricultural and forestry wastes play a very important role. Furfural slag is a by-product of furfural production and is a resource-rich industrial fiber waste. The content of cellulose and lignin in furfural residue is high, and it contains almost no hemicellulose, which makes it a potential raw material for fuel ethanol production. In recent years, the research on effectively degrading furfural slag has been widely concerned by scholars at home and abroad, but the industrial utilization of furfural slag has not been realized yet. This is mainly because the high lignin content in furfural residue leads to low enzymatic hydrolysis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/645
CPCC12P7/10Y02E50/10
Inventor 蒋建新郑天然李鹏飞
Owner BEIJING FORESTRY UNIVERSITY
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