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Glycopeptide having function of resisting depression and preparation method and application of glycopeptide

An antidepressant and glycopeptide technology, applied in the biological field, can solve problems such as cognitive impairment and sexual dysfunction

Active Publication Date: 2019-08-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A large amount of research data shows that the formation mechanism of depression is caused by various factors. Modern clinical treatment of depression mainly uses monoamine oxidase inhibitors, norepinephrine inhibitors and 5-hydroxytryptamine inhibitors, etc., accompanied by many side effects, such as: Sleep disorders, fatigue, cognitive impairment, sexual dysfunction, etc., bring a lot of trouble to patients

Method used

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  • Glycopeptide having function of resisting depression and preparation method and application of glycopeptide
  • Glycopeptide having function of resisting depression and preparation method and application of glycopeptide
  • Glycopeptide having function of resisting depression and preparation method and application of glycopeptide

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1, preparation of ganoderma lucidum glycopeptide (glycopeptide PGL)

[0046] 1. Preparation of Crude Extract

[0047]Add Ganoderma lucidum spore powder to deionized water, the mass ratio of Ganoderma lucidum spore powder to deionized water is 1:40, extract at 90°C for 4 hours, repeat the extraction three times, and combine the extracts to obtain Ganoderma lucidum water extract. Use the Seveage method to remove the protein in the water extract of Ganoderma lucidum to obtain an extract that removes the protein. The specific method is as follows: Sevag reagent that is 1 / 3 volume of the water extract of Ganoderma lucidum (by chloroform and n-butanol according to the volume of 4:1 (formed by mixing) into the water extract of Ganoderma lucidum, vortexed for 5 minutes, centrifuged at 4500g for 15 minutes, absorbed the supernatant, and removed the gelatinous precipitate produced by the free protein. Add Sevag reagent which is 1 / 3 of the volume of the supernatant to ...

Embodiment 2

[0102] Example 2. Glycopeptide PGL has antidepressant function

[0103] 1. Evaluation of anti-acute depression function

[0104] Male C57BL / 6 mice (8 weeks old, weighing 22-28 g) were purchased from Shanghai Slack Experimental Animals Co., Ltd., and the animals started the experiment after a one-week adaptation period. Mice were randomly divided into 5 groups, 10 animals in each group, respectively blank control group, 100mg / kg PGL group, 200mg / kg PGL group, 400mg / kg PGL group and positive control group. Each mouse in the 100mg / kg PGL group was intragastrically administered the glycopeptide PGL of Example 1 so that the dosage of the glycopeptide PGL was 100mg / kg body weight; each mouse in the 200mg / kg PGL group was administered once a time The glycopeptide PGL of Example 1 was administered intragastrically, so that the dosage of the glycopeptide PGL was 200 mg / kg body weight; each mouse in the 400 mg / kg PGL group was intragastrically administered the glycopeptide PGL of Examp...

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Abstract

The invention discloses glycopeptide having the function of resisting depression and a preparation method and application of the glycopeptide. The glycopeptide having the function of resisting depression is from glossy ganoderma, the N-terminal sequence of the glycopeptide is as shown in a sequence 1 in a sequence table, and the glycopeptide has 4 peptide segments as shown in 1-4 in the sequence table. The glycopeptide can reduce the tail suspension fixing time of mice, reduce the swimming dead time of the mice, increase the sucrose preference ratio of depression mice, increase the level of 5-hydroxytryptamine in prefrontal cortex of the depression mice, increase the level of noradrenaline in the prefrontal cortex of the depression mice, increase the expression level of BDNF protein in theprefrontal cortex of the depression mice and reduce the content of corticosterone of the depression mice. The glycopeptide can be used for preventing and / or treating depression.

Description

technical field [0001] The invention relates to a glycopeptide with antidepressant function in the biological field, its preparation method and application. Background technique [0002] Depression is one of the most common mental illnesses, defined as low mood, lack of initiative, low self-esteem, strong feelings of guilt, and suicidal tendencies, and it includes emotional, cognitive, and physical symptoms. The global disease epidemiology report shows that depression affects more than 3.5 billion people worldwide, with an estimated prevalence of 5.8% in men and 9.5% in women, and more than 10% of the world's population is experiencing emotional anxiety and The torment of chronic chronic stress. [0003] A large amount of research data shows that the formation mechanism of depression is caused by various factors. Modern clinical treatment of depression mainly uses monoamine oxidase inhibitors, norepinephrine inhibitors and 5-hydroxytryptamine inhibitors, etc., accompanied b...

Claims

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Application Information

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IPC IPC(8): C07K14/375C07K1/14C07K1/30C07K1/18A61K38/16A61P25/24A23L33/18A23L31/00
CPCA23L31/00A23L33/18A23V2002/00A61K36/074A61K38/00A61P25/24C07K14/375A23V2200/322A23V2250/55
Inventor 赵爽高宜王贺祥马树贵荣成博高琪苏哲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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