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Preparation process of high-quality heparin sodium

A preparation process, the technology of heparin sodium, which is applied in the field of preparation process of high-quality heparin sodium, can solve the problems of low yield and achieve the effects of simple operation, reduced waste water treatment cost and high purification efficiency

Active Publication Date: 2019-08-30
GUANGYUAN HAITIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to refine heparin sodium through oxidative decolorization, and the yield is low. The purpose is to provide a preparation process of high-quality heparin sodium, which solves the problem of low yield of heparin sodium refined through oxidative decolorization in the traditional process

Method used

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  • Preparation process of high-quality heparin sodium
  • Preparation process of high-quality heparin sodium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of preparation technology of high-quality heparin sodium of the present invention, comprises the following steps:

[0031] S1. Dissolving the crude sodium heparin with water to obtain a crude sodium heparin aqueous solution, adding a complex protein precipitant to the crude sodium heparin aqueous solution for protein precipitation, and separating the precipitate after standing for 20-40 minutes;

[0032] S2. Filter the crude sodium heparin aqueous solution after separating the precipitate in step S1, lower the temperature to 10-15°C, add 85-95% ethanol to the crude sodium heparin aqueous solution at 10-15°C until the precipitate precipitates , let stand for 12-24 hours, centrifuge and dry to obtain heparin sodium wet product;

[0033] S3, drying the wet product of heparin sodium at 30-35°C to obtain a sub-fine product of heparin sodium;

[0034] S4, adding water to dissolve the sub-fine heparin sodium, adjusting the pH of the solution to 8-9, adding a protein co...

Embodiment 2

[0040] Based on Example 1, the composite protein precipitation agent includes the following components in parts by weight: 10 parts of 5-sulfosalicylic acid, 3 parts of trichloroacetic acid, 2 parts of acetonitrile, 5 parts of ethyl acetate, 10 parts of polyacrylic acid , 0.8 parts of sodium acetate buffer.

[0041]In the present invention, various components capable of precipitating proteins and having good compatibility are compounded, the effect of protein precipitation is good, and the acidity of the protein precipitating agent can also be reduced; the present invention optimizes the proportioning range of each component, and the pH of the composite protein precipitating agent is to Control it at 4-5, and it will not cause damage to the chromatographic column.

Embodiment 3

[0043] Based on Example 1, the composite protein precipitation agent includes the following components in parts by weight: 15 parts of 5-sulfosalicylic acid, 8 parts of trichloroacetic acid, 6 parts of acetonitrile, 10 parts of ethyl acetate, 20 parts of polyacrylic acid , 5 parts sodium acetate buffer.

[0044] In the present invention, various components capable of precipitating proteins and having good compatibility are compounded, the effect of protein precipitation is good, and the acidity of the protein precipitating agent can also be reduced; the present invention optimizes the proportioning range of each component, and the pH of the composite protein precipitating agent is to Control it at 4-5, and it will not cause damage to the chromatographic column.

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Abstract

The invention discloses a preparation process of high-quality heparin sodium, comprising the following steps of adding water to dissolve crude heparin sodium to obtain a crude heparin sodium water solution, adding a compound protein precipitant into the crude heparin sodium water solution for protein precipitation, standing for 20 to 40 min and then separating out the precipitate; filtering and cooling the crude heparin sodium water solution with the precipitate being separated out, adding ethyl alcohol into the crude heparin sodium water solution until the precipitate is separated out, then performing centrifuge dripping to obtain a heparin sodium wet product; drying the heparin sodium wet product to obtain inferior-quality heparin sodium; adding water to dissolve the inferior-quality heparin sodium, adding a protein complex enzyme preparation into the inferior-quality heparin sodium solution, centrifuging and filtering to obtain inferior-quality heparin sodium liquor; performing circulating adsorption on the inferior-quality heparin sodium liquor with dynamic adsorbent resin, eluting the resin, adding ethyl alcohol into the eluate, filtering, cooling and drying to obtain the high-quality heparin sodium. The preparation process of the high-quality heparin sodium provided by the invention improves the productive rate of the high-quality heparin sodium.

Description

technical field [0001] The invention relates to a preparation process of heparin sodium, in particular to a preparation process of high-quality heparin sodium. Background technique [0002] Heparin sodium is a mucopolysaccharide sulfate-based anticoagulant drug, which is widely used in clinical use to prevent and treat various cardiovascular and cerebrovascular diseases. It has always been the most effective anticoagulant drug, especially for arteriovenous thrombosis and blood clots. curative effect. Heparin sodium widely exists in the intestinal mucosa, lung, and liver of mammals, especially in the pig small intestinal mucosa, and the heparin extracted from the healthy pig small intestinal mucosa is basically consistent with the molecular structure of heparin in the human body. The production method of heparin sodium in the prior art mainly uses fresh porcine small intestinal mucosa as a raw material, and the crude heparin sodium is obtained through cleaning, enzymatic ext...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0003C08B37/0075
Inventor 李海生
Owner GUANGYUAN HAITIAN IND