Method for producing p-hydroxyphenylethanol and use of penicillium chrysogenum
A technology for p-hydroxyphenethyl alcohol and Penicillium chrysogenum, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., to achieve the effect of simple process
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Embodiment 1
[0043] The production method of p-hydroxyphenethyl alcohol comprises the steps:
[0044] (1) Steps in which Penicillium chrysogenum DXY-1 is fermented and cultivated to obtain a fermented liquid
[0045] Inoculate the plate-activated Penicillium chrysogenum DXY-1 (derived from the sediment in the Taiwan Strait) into the culture solution, and culture it with shaking at 28°C and 150rmp for 72 hours to obtain the seed culture solution; then transfer the seed culture solution to the culture medium On this basis, cultured with shaking at 28° C. and 150 rpm for 7 days to obtain a fermentation broth. The inoculum amount of the seed culture solution is 5wt% of the weight of the culture medium.
[0046] (2) The step of extracting the fermented liquid to obtain the extract
[0047] Break the mycelium balls in the fermentation broth, then add ethyl acetate to extract 3 times, combine the supernatants, and use a rotary evaporator to concentrate to obtain the extract; the amount of ethyl...
experiment example
[0051] The structure of the p-hydroxyphenethyl alcohol product obtained in Example 1 was identified.
[0052] The ultraviolet spectrum shows that the characteristic absorption peak of benzene appears at a wavelength of about 275nm, and it is speculated that the compound may contain a benzene ring. The K absorption band formed by the conjugated double bond system appears at a wavelength of 220nm, indicating that the compound contains more double bonds.
[0053] H NMR spectrum ( 1 H NMR) gave 10 hydrogen signals. An active hydrogen signal (δ H 9.11, s, 4-OH), four benzene ring hydrogen signals appear in the aromatic region (δ H 6.99, d, H-4; δ H 6.99, d, H-8; δ H 6.63, d, H-5; δ H 6.63, d, H- 7;), overlapping in pairs and splitting into d peaks, indicating that there may be a 1,4-disubstituted benzene ring structure, and a methylene signal appears in the high field area (δ H 2.60, t, H-2), an oxygen-linked methylene signal (δ H 3.50, q, H-1) and an active hydrogen ...
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