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Method for rapidly detecting quality change of myofibrillar protein containing phlorotannin in UVA irradiation process

A technology of myofibrillar protein and irradiation process, which is applied in the field of rapid detection of quality change of myofibrillar protein containing brown algae polyphenols in the process of UVA irradiation, and can solve complex problems, low sensitivity and accuracy, and interference measurement results Accuracy and precision and other issues to achieve the effect of accurate methods

Active Publication Date: 2019-09-10
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing technology has the problems of low sensitivity and precision. At the same time, the final products of lipid peroxidation are more complicated. In addition to malondialdehyde, there are other aldehyde products, which will interfere with the accuracy and precision of the measurement results.

Method used

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  • Method for rapidly detecting quality change of myofibrillar protein containing phlorotannin in UVA irradiation process
  • Method for rapidly detecting quality change of myofibrillar protein containing phlorotannin in UVA irradiation process
  • Method for rapidly detecting quality change of myofibrillar protein containing phlorotannin in UVA irradiation process

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Embodiment 1

[0055] A method for rapidly detecting the quality change of myofibrillar protein containing brown algae polyphenols during UVA irradiation, comprising steps:

[0056] S1, extract myofibrillar protein from mackerel meat, use pH 6, 25mM phosphate buffer (containing 0.6MNaCl), prepare the myofibrillar protein solution that concentration is 50mg / mL; In described myofibrillar protein solution Add undaria sporophyll polyphenols to obtain a myofibrillar protein solution sample containing wakame sporophyll polyphenols; based on the total amount of myofibrillar protein in the myofibrillar protein solution, every gram of myofibrillar protein is added 625 μmol of wakame sporophyll polyphenol; the free radical scavenger POBN is added to the myofibrillar protein solution sample containing wakame spore leaf polyphenol, so that the final concentration of the free radical scavenger POBN is 40mM, and a mixed sample is obtained;

[0057] S2. UVA irradiation is performed on the mixed sample obta...

Embodiment 2

[0061] A method for rapidly detecting the quality change of myofibrillar protein containing brown algae polyphenols during UVA irradiation, comprising steps:

[0062] S1, extract myofibrillar protein from grass carp meat, use pH6, 25mM phosphate buffer (containing 0.6M NaCl), prepare the myofibrillar protein solution that concentration is 70mg / mL; Add to described myofibrillar protein solution Add kelp polyphenols to obtain a myofibrillar protein solution sample containing kelp polyphenols; based on the total amount of myofibrillar proteins in the myofibrillar protein solution, add 25 μmol of kelp polyphenols per gram of myofibrillar protein; The free radical scavenger POBN is added to the myofibrillar protein solution sample containing laminaria polyphenols, so that the final concentration of the free radical scavenger POBN is 30mM, and a mixed sample is obtained;

[0063] S2. UVA irradiation is performed on the mixed sample obtained in step S1 at 4°C; wherein, the UVA irradi...

Embodiment 3

[0067] A method for rapidly detecting the quality change of myofibrillar protein containing brown algae polyphenols during UVA irradiation, comprising steps:

[0068] S1, extract myofibrillar protein from mackerel meat, use pH6, 25mM phosphate buffer (containing 0.6MNaCl), prepare the myofibrillar protein solution that concentration is 35mg / mL; In described myofibrillar protein solution Add undaria sporophyll polyphenols to obtain a myofibrillar protein solution sample containing wakame sporophyll polyphenols; based on the total amount of myofibrillar protein in the myofibrillar protein solution, every gram of myofibrillar protein is added 125 μmol of wakame sporophyll polyphenols; the free radical scavenger POBN is added to the myofibrillar protein solution sample containing wakame sporophyll polyphenols, so that the final concentration of the free radical scavenger POBN is 20mM to obtain a mixed sample;

[0069] S2. UVA irradiation is performed on the mixed sample obtained i...

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Abstract

The invention discloses a method for rapidly detecting the quality change of myofibrillar protein containing phlorotannin in a UVA irradiation process. The method comprises the steps of: S1, taking amyofibrillar protein solution sample containing phlorotannin, and adding a radical scavenger a-(4-pyridyl-1-oxygen)-N-tert-butyl nitroketone, wherein the final concentration is 20-50 mM; S2, performing UVA irradiation on the mixed sample obtained in the step S1; and S3, absorbing the sample subjected to UVA irradiation by using a capillary tube, and performing electron spin resonance detection. According to the method in the invention, oxidation material generation of the myofibrillar protein containing phlorotannin in the UVA irradiation process is detected by adoption of an ESR technology; and a relatively rapid and accurate method and clear specifications are provided for quality detection in myofibrillar protein processing.

Description

technical field [0001] The invention belongs to the technical field of food quality detection, and in particular relates to a method for rapidly detecting the quality change of myofibrillar protein containing brown algae polyphenols during UVA irradiation. Background technique [0002] Phlorotannin is a kind of phenolic compound extracted from brown algae, which is a derivative of phloroglucinol, which has biological activities such as anti-oxidation, anti-allergy, anti-tumor, immunity, and scavenging free radicals. However, the current industrial utilization of brown algae is mainly to extract alginate, iodine, mannitol and potassium chloride from it, and rarely involves the extraction and utilization of brown algae polyphenols. [0003] Irradiation technology has been applied in food preservation, quality and safety control and quality improvement by more and more countries. The UVA band is part of the ultraviolet wavelength division, with a wavelength of 320-420nm. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/10G01N1/28
CPCG01N24/10G01N1/28
Inventor 启航蒋迪时逸馨乔昕昱谷月申平贺宝玉董秀萍姜鹏飞
Owner DALIAN POLYTECHNIC UNIVERSITY
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