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Preparation method of two-aqueous-phase micro liquid droplets with uniform and controllable sizes

A two-phase, micro-droplet technology, applied in the field of droplet microfluidics and microcapsules, can solve problems such as difficult mass industrial production, removal of solid particle templates, complex processes, etc., to achieve precise operation control, Controllable capsule size and uniform capsule size

Inactive Publication Date: 2019-09-13
INT ACAD OF OPTOELECTRONICS AT ZHAOQING SOUTH CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the disadvantages of complex process, long time-consuming, and the need to remove solid particle templates, it is difficult to mass-produce industrially.

Method used

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  • Preparation method of two-aqueous-phase micro liquid droplets with uniform and controllable sizes
  • Preparation method of two-aqueous-phase micro liquid droplets with uniform and controllable sizes
  • Preparation method of two-aqueous-phase micro liquid droplets with uniform and controllable sizes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The PDMS chip microfluidic device described in Comparative Example 1 was used to prepare two-phase aqueous microdroplets.

[0048] The attached drawings are the same figure 1 , and the labels are as follows: 1, inner phase syringe, 2, middle phase syringe, 3, outer phase syringe, 4, silica gel connecting tube, 5, upper part of PDMS chip, 6, slide glass, 7, mechanical wave vibration generator.

[0049] With 15% dextran aqueous solution as the inner phase, 17% polyethylene glycol aqueous solution as the middle phase and the outer phase, draw the above aqueous solution with a syringe, connect it to the corresponding feed port with a silicone tube, and use the pump to set the liquid flow rate of each phase , the flow rate of the outer phase is 120 μL / h, the flow rate of the middle phase is 120 μL / h, and the flow rate of the inner phase is 30 μL / h. The silicone tube of the inner phase is fixed on a mechanical wave vibration generator, so that the silicone tube follows the me...

Embodiment 2

[0051] use as Image 6 , Figure 7 with Figure 8 The glass capillary microfluidic device shown prepares aqueous two-phase microdroplets. The inner phase flows out from the tip of the round glass capillary at the left end, the middle phase flows out from the gap between the round glass capillary tube at the left end and the square glass capillary tube, the outer phase flows out from the gap between the round glass capillary tube at the right end and the square glass capillary tube, and finally undrawn from the right end together Outflow from a round glass capillary. The circular glass capillary at the left end of the square capillary was inserted into a tapered end drawn to a diameter of 30 μm, and the circular glass capillary at the right end of the square capillary was not drawn. The distance between two circular glass capillaries is about 1000 μm.

[0052] Reference numerals: 1, inner phase syringe, 2, middle phase syringe, 3, outer phase syringe, 4, silica gel connecti...

Embodiment 3

[0055] The glass capillary microfluidic device described in Example 2 was used to prepare two-phase aqueous microdroplets.

[0056] With 15% dextran aqueous solution as the inner phase, 17% polyethylene glycol aqueous solution as the middle phase and the outer phase, draw the above aqueous solution with a syringe, connect it to the corresponding feed port with a silicone tube, and use the pump to set the liquid flow rate of each phase , the flow rate of the outer phase is 1000 μL / h, the flow rate of the middle phase is 1000 μL / h, and the flow rate of the inner phase is 30 μL / h. The silicone tube of the inner phase is fixed on a mechanical wave vibration generator, so that the silicone tube follows the mechanical wave vibration generator Vibrate at a certain frequency, the vibration frequency is 25Hz, to obtain monodisperse two-phase micro-droplets with a diameter of 88 μm, see Figure 10 .

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Abstract

The invention discloses a preparation method of micro liquid droplets with uniform and controllable sizes based on an ultra-low interfacial tension two-aqueous-phase system. The method specifically comprises the following steps: a glucan aqueous solution is used as an inner phase, a polyethylene glycol aqueous solution is used as a middle phase and an outer phase, the three phases are merged in amicrofluidic device to form a laminar flow, and the monodisperse two-aqueous-phase liquid droplets are generated by utilizing action of mechanical wave vibration; and in addition, the obtained two-aqueous-phase liquid droplets are used as a template, polyelectrolytes in the inner phase and the outer phase meet at the liquid droplet interface and complexed through electrostatic force, and thereforepolyelectrolyte microcapsules are prepared. According to the method provided by the invention, the two-aqueous-phase liquid droplets prepared by the method have good monodispersity, and the method can effectively control the sizes of the liquid droplets by changing flow speeds of an inner-phase fluid, a middle-phase fluid and an outer-phase fluid and frequency and amplitude of applied vibration;and compared with a traditional method for preparing capsules by using oil / water liquid droplets, the method provided by the invention uses the two-aqueous-phase liquid droplets as templates to prepare the capsules without using organic solvents, and the two-aqueous-phase liquid droplets have higher biocompatibility and have wider application in the fields of food, cosmetics, medicine, biologicalmicroreactors and sensing analysis testing.

Description

technical field [0001] The invention relates to the field of droplet microfluidic technology and microcapsule technology, in particular to a method for uniformly and controllably preparing microdroplets and microcapsules based on a two-phase fluid system (two-phase fluid system) with extremely low interfacial tension. Background technique [0002] The two-phase aqueous system is usually composed of two aqueous polymer solutions or an aqueous polymer solution and a salt solution. When the concentration of the two solutions exceeds a certain threshold, the two mixed solutions will spontaneously separate into phases, forming a two-phase system in which the upper and lower phases are aqueous solutions, that is, a two-phase aqueous system. [0003] For example, a certain concentration of polyethylene glycol and dextran aqueous solution can spontaneously separate phases to form an aqueous two-phase system. Due to the good biocompatibility and selective distribution of this system,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F13/00B01L3/00B01J13/02
CPCB01L3/5027B01J13/02B01L2300/0861B01F33/3021
Inventor 水玲玲邹艺莹金名亮
Owner INT ACAD OF OPTOELECTRONICS AT ZHAOQING SOUTH CHINA NORMAL UNIV
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