Method for predicting joint toxicity interaction of typical antibiotics and pesticides

Abstract

The invention discloses a method for predicting the joint toxicity interaction of typical antibiotics and pesticides. The method designs a binary mixed system of antibiotics and pesticides by using a direct equipartition ray means, is provided with five rays with different concentration ratios, establishes toxicity microplate analysis of three mixed cultured green algae, and tests the joint toxicity of the binary mixture of antibiotics and pesticides to the mixed cultured green algae. The method utilizes the concentration addition and independent action model to analyze the toxicity interaction of the mixture to the mixed cultured green algae. The method not only combines the situation of the symbiosis of various aquatic algae in a natural water body, but also combines the situation that pollutants generally exist in a mixture form in an actual environment, thereby evaluating the potential threat of the pollutants in the actual environment to the environment and the ecology more objectively and comprehensively. In addition, compared with a standard cone flask green algae toxicity test, the method has the advantages of simple and rapid test, less required sample volume and convenience for multiple parallel toxicity tests by utilizing a 96-hole microplate light absorption method.

Description

technical field [0001] The invention belongs to the field of environmental toxicology, and particularly relates to a method for predicting two typical antibiotics (soil (OXY) and ciprofloxacin (CIP)), and a typical triazole fungicide pesticide (triadimefon (SZT)) combined toxic interaction method. Background technique [0002] Algae are primary producers of aquatic ecosystems, play an extremely important role in the balance and stability of water bodies, and are important indicators reflecting the quality of water bodies. Selenastrum capricornutum, Scenedesmus obliquus, and Chlorella pyrenoidosa are three common green algae in freshwater. Because of their sensitivity, they are often used as indicators for water safety assessment. The experimental material is also the standard algae species for water environmental toxicology tests. In natural water bodies, there is no monopoly of a single algae species, and there is a symbiotic situation of multiple algae. The study of the ...

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Problems solved by technology

[0004] At present, a single algae species under pure culture conditions has been widely used to conduct studies on the toxicity and safety evaluation of a single antibiotic and pesticide. However, in view of the fact that in natural water bodies, there is no monopoly of a single algae species, and there is a symbiosis of multiple algae. At the same time, in the actual environment, it is impossible for a single chemical substance to exist in the form of a mixture, and there are few reports on the combined toxicity of antibiotics and pesticides using a variety of algal species under mixed culture conditions.

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