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SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof

An eye muscle area and molecular marker technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of restricting the application of molecular markers for complex traits and large confidence regions, and achieve increasing core competitiveness, increasing eye muscle area, and speeding up breeding. effect of the process

Active Publication Date: 2019-09-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the candidate gene method has the advantages of simple method and convenient operation, it can only target genes with known biological functions; while the confidence region of the candidate gene identified by the QTL mapping method is also large, which greatly limits the ability of complex trait molecules Application of markers in livestock genetics and breeding

Method used

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  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof
  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof
  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Experimental animals

[0037] The group of experimental pigs used in the present invention is 3916 purebred American Durocs from the Breeding Pig Branch of Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.

[0038] In this experiment, Chinese and American Duroc pigs were selected from this resource group. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0039] (2) Sample collection

[0040] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0041] (3) 50K SNP typing in the whole pig genome

[0042] The ear tissue or docked tail tissue collected from each individual of the 3916 Duroc breeding pigs selected from the above-mentioned resource groups, the whole genome DNA was extracted by the standard phe...

Embodiment 2

[0059] Example 2 Target DNA sequence amplification and sequencing

[0060] (1) Primer design

[0061] The DNA sequence of SEQ ID NO: 1 on pig chromosome 6 was downloaded from Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers. The DNA sequences of the designed primers are as follows:

[0062] P001-F: 5'-ACTCTTGCCTCCGACTTCTC-3',

[0063] P002-R: 5'-AGACCTGGTGACATAGTTGATGA-3';

[0064] (2) PCR amplification

[0065] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0066] (3) DNA sequence determination

[0067] DNA sequence sequencing identification: carried out in Shenzh...

Embodiment 3

[0070] Example 3 SNP site g.333 T>C effect analysis of molecular markers

[0071] According to Tables 1 to 3, it can be seen that for lean meat percentage, eye muscle area and eye muscle thickness, the effect of SNP locus g. 1.28 and 0.76. Therefore, through molecular marker-assisted selection and gradually eliminating pigs with genotype CC in the population, the allele frequency of the allele T can be significantly increased, and the lean meat percentage, eye muscle area, and eye muscle thickness of the population can be improved. Pig growth and production performance are improved, and more high-quality commercial lean pork can be produced to meet people's demand for high-quality pork, thereby driving the growth of pork sales, which will bring huge economic benefits to enterprises.

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Abstract

The invention belongs to the fields of molecular biotechnology and molecular marker technology, and in particular, relates to an SNP molecular marker related to the lean meat rate, eye muscle area and eye muscle thickness on a swine chromosome 6 and an application thereof. The SNP locus of the molecular marker corresponds to the T>C mutation at a 4735966th site on chromosome 6 of a reference sequence of International Swine Genome 11.1. The invention also provides a primer pair for identifying the SNP molecular marker related to the lean meat rate, eye muscle area and eye muscle thickness on the swine chromosome 6. By optimizing a dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the lean meat rate of breeding pigs, the eye muscle area and the eye muscle thickness are increased, excellent breed pigs with the three traits are synergically bred, the progress of genetic improvement of pigs is accelerated, and the economic benefits of pig breeding are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker on pig chromosome 6 related to lean meat percentage, eye muscle area and eye muscle thickness and its application. Background technique [0002] In recent years, with the improvement of people's living standards and the demand for pork consumption, lean pork has occupied a large market share of pork consumption. Therefore, the breeding of high-quality lean pigs has become the focus of breeding work, and the relevant traits that affect pig growth are important breeding goals. The lean meat percentage of pigs refers to the percentage of lean meat weight in pigs to the whole carcass weight, which is an important indicator to measure the growth performance of pigs. For a long time, thanks to people's continuous pursuit of lean meat, the lean meat rate has been significantly improved. Eye muscle area and eye mus...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 吴珍芳杨杰庄站伟蔡更元郑恩琴徐铮
Owner SOUTH CHINA AGRI UNIV
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