SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof

An eye muscle area and molecular marker technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of restricting the application of molecular markers for complex traits and large confidence regions, and achieve increasing core competitiveness, increasing eye muscle area, and speeding up breeding. effect of the process

Active Publication Date: 2019-09-20
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the candidate gene method has the advantages of simple method and convenient operation, it can only target genes with known biological functions; while the confidence region of t

Method used

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  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof
  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof
  • SNP molecular marker related to lean meat rate, eye muscle area and eye muscle thickness on swine chromosome 6 and application thereof

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Experimental program
Comparison scheme
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Example Embodiment

[0035] Example 1

[0036] (1) Experimental animals

[0037] The experimental pig group used in the present invention is 3916 purebred American Duroc of the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch.

[0038] In this experiment, a total of Chinese and American Duroc breeding pigs were selected from this resource group. The pigs were free to eat and drink. The entire feeding method and feeding conditions were always consistent, which was a conventional method.

[0039] (2) Sample collection

[0040] Collect the piglet tail and ear tissues, soak them in a 75% ethanol solution, and store them in a refrigerator at -20°C for later use.

[0041] (3) 50K SNP type of pig whole genome

[0042] Ear tissues or tail tissues collected from each individual of the 3916 Duroc breeding pigs selected from the above resource groups were used to extract the whole genome DNA using the standard phenol-chloroform method, and each one was accurately meas...

Example Embodiment

[0059] Example 2 Amplification and sequencing of target DNA sequence

[0060] (1) Primer design

[0061] Download the DNA sequence of SEQ ID NO:1 on chromosome 6 of pigs through Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers. The DNA sequence of the designed primer is as follows:

[0062] P001-F: 5’-ACTCTTGCCTCCGACTTCTC-3’,

[0063] P002-R: 5’-AGACCTGGTGACATAGTTGATGA-3’;

[0064] (2) PCR amplification

[0065] Add 1 μL of DNA template, 3.4 μL of double-distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R to the 10 μL reaction system. The PCR reaction conditions were: after pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 45s, 35 cycles, and finally extension at 72°C for 5 minutes.

[0066] (3) DNA sequence determination

[0067] DNA sequence identification: conducted in Shenzhen Huada Gene Technol...

Example Embodiment

[0070] Example 3 Molecularly labeled SNP site g.333 T> C effect analysis

[0071] According to Tables 1 to 3, it can be seen that for lean meat rate, eye muscle area and eye muscle thickness, SNP site g.333T> The effect of C predominant allele (TT) was significantly increased by 0.62, 1.28 and 0.76 compared with the average value of CC phenotype. Therefore, through molecular marker-assisted selection, the gradual elimination of pigs with genotype CC in the population can significantly increase the allele frequency of the allele T, and increase the lean meat rate, eye muscle area, and eye muscle thickness of the population. The growth and production performance of pigs can be improved, and more high-quality commodity lean-type pork can be produced to meet people's demand for high-quality pork, thus driving the growth of pork sales, which will bring huge economic benefits to the enterprise.

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Abstract

The invention belongs to the fields of molecular biotechnology and molecular marker technology, and in particular, relates to an SNP molecular marker related to the lean meat rate, eye muscle area and eye muscle thickness on a swine chromosome 6 and an application thereof. The SNP locus of the molecular marker corresponds to the T>C mutation at a 4735966th site on chromosome 6 of a reference sequence of International Swine Genome 11.1. The invention also provides a primer pair for identifying the SNP molecular marker related to the lean meat rate, eye muscle area and eye muscle thickness on the swine chromosome 6. By optimizing a dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the lean meat rate of breeding pigs, the eye muscle area and the eye muscle thickness are increased, excellent breed pigs with the three traits are synergically bred, the progress of genetic improvement of pigs is accelerated, and the economic benefits of pig breeding are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker on pig chromosome 6 related to lean meat percentage, eye muscle area and eye muscle thickness and its application. Background technique [0002] In recent years, with the improvement of people's living standards and the demand for pork consumption, lean pork has occupied a large market share of pork consumption. Therefore, the breeding of high-quality lean pigs has become the focus of breeding work, and the relevant traits that affect pig growth are important breeding goals. The lean meat percentage of pigs refers to the percentage of lean meat weight in pigs to the whole carcass weight, which is an important indicator to measure the growth performance of pigs. For a long time, thanks to people's continuous pursuit of lean meat, the lean meat rate has been significantly improved. Eye muscle area and eye mus...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 吴珍芳杨杰庄站伟蔡更元郑恩琴徐铮
Owner SOUTH CHINA AGRI UNIV
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