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A method for co-producing cellobiase and chitin

A technology of cellobiase and chitin, applied in the field of bioengineering, can solve the problem that cellobiase and chitin cannot be obtained at the same time, achieve the effects of enriching product varieties, sharing production costs, and reducing the pressure of environmental protection treatment

Active Publication Date: 2020-07-21
ZHONGRONG TECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the strain of this patent cannot obtain cellobiase and chitin at the same time

Method used

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  • A method for co-producing cellobiase and chitin
  • A method for co-producing cellobiase and chitin

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Prepare basal medium, glucose 10g / L, cellulose powder 20g / L, peptone 10g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.2g / L, pH5.0, in a 10L fermenter at 121-126 Sterilize at ℃ for 30min.

[0034] Add calcium citrate of different concentrations in the basal medium, inoculate Aspergillus niger with 10% inoculum size (v / v) again, carry out fermentation in the fermentor, the fermentor medium filling capacity is 7L, and fermentation condition is: Temperature 30°C (automatic control), pH 5.0 (automatic control by feeding 20% ​​ammonia solution), stirring speed 350r / min, air flow 18L / min, tank pressure 0.04MPa.

[0035] In this example, the Aspergillus niger used was the Aspergillus niger purchased from China General Microorganism Culture Collection Center with the preservation number CGMCC NO.3.11447.

[0036] After the fermentation is completed, pass the fermented liquid through a 0.2 μm microfiltration membrane, the permeated liquid is the cellobiose enzyme ...

Embodiment 2

[0043] Prepare basal medium, glucose 10g / L, cellulose powder 20g / L, peptone 10g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.2g / L, pH 5.0, in a 10L fermenter at 121-126°C Sterilize for 30min.

[0044] Add 1.5% calcium citrate in the basal medium, inoculate Aspergillus niger with 10% inoculum size (v / v) again, carry out fermentation in fermenter, fermentation condition is: temperature 30 ℃ (self-control), pH 5.0 ( With 20% ammonia solution feeding automatic control), the stirring speed is 350r / min, the ventilation rate is 18L / min, and the tank pressure is 0.04MPa.

[0045] In this example, the Aspergillus niger used was the Aspergillus niger purchased from China General Microorganism Culture Collection Center with the preservation number CGMCC NO.3.11447.

[0046] After the fermentation is completed, the fermentation liquid is passed through a 0.2 μm microfiltration membrane, and the permeated liquid is the cellobiase liquid, and the retentate is separated from ...

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Abstract

The invention relates to a method for co-producing cellobiase and chitin. The method comprises the step that aspergillus niger is inoculated into a fermentation culture medium added with calcium citrate for fermentation, wherein the obtained fermentation liquor contains cellobiase, and the fermented aspergillus niger body contains chitin. Compared with the prior art, the production promoting factor, namely, calcium citrate, which is suitable for producing chitin is added into the culture medium, and an effective separation and extraction method is used, so that chitin is prepared by utilizing waste thalli produced by fermentation while cellobiase is produced, co-production of cellobiase and high-added-value product chitin is realized, the product variety is enriched, and therefore the production cost of cellobiase is shared; by-product thalli are also utilized, so that the environment-friendly treatment pressure is reduced, and a new direction is provided for the development of the cellobiase industry.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for co-producing cellobiase and chitin. Background technique [0002] At present, countries all over the world are actively researching the use of non-food means to produce biofuels to solve the increasingly serious energy crisis, climate problems and food shortages. Lignocellulose is the most abundant polysaccharide on the earth, and the production of fuel ethanol from lignocellulose has become a hot research field in various countries. [0003] However, due to the dense and complex structure of lignocellulose, most microorganisms cannot use it as a direct carbon source to produce ethanol. Only after it is hydrolyzed into fermentable monosaccharides can it be used by microorganisms. Enzymatic hydrolysis has the advantages of mild reaction conditions, high efficiency, low energy consumption, strong selectivity and good environmental protection effect, especially cellobiase...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12P19/26C12R1/685
CPCC12N9/2437C12P19/26C12Y302/01091
Inventor 杨明李秋园代淑梅
Owner ZHONGRONG TECH CORP LTD
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