Nucleic acid protein complex allosteric microbial whole-cell gas sensor used for heavy metal detection

Abstract

The invention discloses a nucleic acid protein complex allosteric microbial whole-cell gas sensor used for heavy metal detection, which is applicable to detection of the pollution degree of mercury ions in soil, and particularly relates to the microbial gas sensor for mercury ion detection and a use method of the microbial gas sensor. The microbial gas sensor comprises Escherichia coli serving as a host cell to support recombinant plasmid and the recombinant plasmid, wherein the recombinant plasmid is pENTR plasmid containing a mercury resistant system mer promoter, a mercury resistant system regulation gene merR, an ethylene production gene efe and a constitutive promoter serial sequence. The use method of the sensor comprises the steps as follows: a strain recovery method, a sensor detection method and a sample detection method. The sensor has mercury ion response time of 40 h, the minimum detection concentration of 1 mu M and the maximum detection concentration of 100 mu M. The sensor can be widely applied to detection and risk evaluation of mercury ion content in the soil.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a novel microbial whole-cell gas sensor for detecting mercury ions. Background technique [0002] Mercury is a common heavy metal with the element symbol Hg. It is located in the sixth period and Group IIB of the periodic table of chemical elements. It is the only metal that exists in a liquid state at normal temperature and pressure. Mercury is a silvery white shiny heavy liquid, chemically stable, insoluble in acid and alkali. Mercury can evaporate at room temperature, and mercury vapor and mercury compounds are highly toxic (chronic). The use of mercury has a long history and is widely used, but the pollution situation is relatively serious. Mercury metal continues to accumulate in the natural environment, causing water, soil, and air pollution. Like other heavy metals, mercury cannot be metabolized by microorganisms, nor can it participate in the chemical degrada...

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Problems solved by technology

However, these analysis methods are costly and time-consuming, not only require the support of expensive instruments, but also require complex pretreatment processes and large sample volumes, are not suitable for on-site and on-line analysis and testing, and are difficult to adapt to the environment and on-site random inspection of market products , production enterprise self-inspection and product import and export fast customs clearance requirements

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