Talaromycesveerkampii strain for degrading cellulose and adsorbing cadmium and application of strain

A technology for degrading cellulose and yellow-blue-like bacteria, which is applied in the field of environmental management to achieve the effect of repairing heavy metal pollution in soil

Pending Publication Date: 2019-10-01
湖南慧谷农业生态研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report about the yellow-blue bacteria that c

Method used

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  • Talaromycesveerkampii strain for degrading cellulose and adsorbing cadmium and application of strain
  • Talaromycesveerkampii strain for degrading cellulose and adsorbing cadmium and application of strain
  • Talaromycesveerkampii strain for degrading cellulose and adsorbing cadmium and application of strain

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: This embodiment illustrates that the present invention uses culture medium

[0028] (1) Separation medium: CMC medium: sodium carboxymethylcellulose (CMC-Na) 10.0g, KH2PO4 0.25g, potato juice 100mL, agar powder 18.0g, distilled water 1000mL.

[0029] (2) Purification and storage medium: PDA medium: 200 g of potatoes, 20.0 g of glucose, 18.0 g of agar powder, and 1000 mL of distilled water.

[0030] (3) Cadmium ion-containing medium: 200g of potatoes, 20.0g of glucose, 18.0g of agar powder, and 1000mL of distilled water. Add cadmium ion-containing mother liquor (20mg / mL) as needed to prepare solid medium with different concentrations of cadmium ions.

[0031] (4) Screening medium:

[0032] a. Congo red medium: CMC-Na 2.0g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.25g, (NH 4 ) 2 SO 4 1.0g, Congo red 0.1g, agar powder 18.0g, distilled water 1000mL;

[0033] b. Re-screening medium: CMC-Na 5.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, NaNO 3 3.0g, KCl0.5g...

Embodiment 2

[0035] Embodiment 2: the separation and purification of test bacterial strain

[0036] The specific method for the separation and purification of test bacterial strains in the present invention is: adopt the dilution plate coating method, the soil sample is made 10 by gradient dilution with sterile water -3 -10 -5 Bacterial suspension, and coated on the separation medium, placed in a constant temperature incubator at 26-28 ° C for 2-3 days, the isolated different fungal strains were streaked and purified in PDA medium, and the purified Good strains were stored in a refrigerator at 4°C.

[0037] The classification of the strain is named Talaromycesveerkampii (Talaromycesveerkampii) CAF2, its preservation number is: ACCC 32732, and its biological characteristics are: the colony texture is flocculent and fluffy, the edge is white to light yellow, the middle is light green, and produces yellow-green Spores; the optimum culture pH is 6.5; the optimum culture temperature is 28°C; ...

Embodiment 3

[0038] Example 3: Evaluation of cadmium ion tolerance of bacterial strain CAF2

[0039] The cadmium ion tolerance evaluation method of bacterial strain CAF2 of the present invention specifically comprises the following steps:

[0040] (1) Prepare PDA medium containing different cadmium ion concentrations, the concentration is set to 0, 10, 50, 80, 100, 150, 200 mg / L;

[0041] (2) Use a 5mm puncher to make bacterial cakes from the bacterial strain, inoculate it on the plate medium described in step (1) under aseptic conditions, and cultivate it in a constant temperature incubator at 26-28°C for 3-5 days to observe the growth of the bacterial strain. growing situation.

[0042] Strain CAF2 grows well on a plate with a cadmium ion concentration of 0-100 mg / L, but growth is completely inhibited on a plate with a cadmium ion concentration of 150 mg / L, so the cadmium ion concentration of the strain is tolerant The range is 0-100mg / L.

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Abstract

The invention provides a talaromycesveerkampii strain for degrading cellulose and adsorbing cadmium. The strain is classified and named as talaromycesveerkampii CAF2, and the preservation number of the strain is ACCC 32732. The cadmium ion concentration tolerance range of the talaromycesveerkampii strain is 0-100 mg/L. The talaromycesveerkampii strain can produce cellulase and has a good degradingeffect on rice straw, and when the strain is applied to degrading the rice straw, in the 15 th day, half of the rice straw is degraded; meanwhile, the strain has the capability of adsorbing heavy metal cadmium, and when the strain is applied to cadmium-polluted soil treatment, in the 30 th day, the adsorption rate of the talaromycesveerkampii strain on cadmium exceeds 74%. The strain is applied to bioremediation of cadmium-polluted rice fields, and can achieve the effect of solving soil heavy cadmium metal pollution while the rice straw is degraded.

Description

technical field [0001] The invention relates to the technical field of environmental treatment, in particular to a cyanobacterium strain capable of degrading cellulose and adsorbing cadmium and its application. Background technique [0002] With the rapid development of industry, soil heavy metal pollution is becoming more and more serious. The common heavy metals in soil pollution mainly include Hg, Cd, Pb, As, etc. As these heavy metals migrate, transform, and accumulate in the ecological environment, when they reach a certain limit, they will endanger animals, plants, and humans through the food chain cycle. life and health. [0003] Chinese patent CN 102286383A discloses the application of a yellow cyanobacterium in the control of plant pathogens. The strain can inhibit a variety of pathogenic bacteria, such as peanut net spot fungus, tomato early blight fungus, Rhizoctonia solani, corn sheath blight fungus , apple rot fungus, tobacco red spot fungus, etc.; in addition...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C05F11/08C05F11/00C12R1/645
CPCB09C1/105C05F11/08C05F11/00C12R2001/645C12N1/145
Inventor 张冬雪郭帅谭石勇文亚雄刘备罗志威
Owner 湖南慧谷农业生态研究院有限公司
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