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Hybrid peptide having functions of detoxification, anti-inflammatory, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and application thereof

A technology of hybrid peptides and polypeptides, which is applied in the field of genetic engineering and biological preparations, can solve problems such as unsuitable for continuous use, achieve strong anti-inflammatory activity, inhibit cell apoptosis and intestinal barrier damage, and reduce expression levels

Active Publication Date: 2019-10-08
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current antibiotic anti-infection and glucocorticoid anti-inflammatory drugs have obvious defects and should not be used continuously

Method used

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  • Hybrid peptide having functions of detoxification, anti-inflammatory, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and application thereof
  • Hybrid peptide having functions of detoxification, anti-inflammatory, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and application thereof
  • Hybrid peptide having functions of detoxification, anti-inflammatory, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Design and acquisition of embodiment 1 hybrid peptide C-L

[0044] By studying the sequence, structure and the relationship between sequence structure and function of polypeptide LL-37 and cecropin A, the protein molecular design technology was used to carry out polypeptide Cecropin A (its amino acid sequence is shown in SEQ ID NO.2) and LL- 37 (whose amino acid sequence is shown in SEQ ID NO.3) to obtain hybrid peptide C-L, whose amino acid sequence is shown in SEQ ID NO.1.

[0045] Myeloid differentiation protein-2 (MD-2) binds to TLR4 (Toll receptor 4) to form a TLR4 / MD2 complex receptor. LPS mainly binds to TLR4 / MD2 on the surface of the cell membrane to activate downstream inflammatory signaling pathways such as NF-κB and MAPK, leading to inflammation outbreaks. Therefore, TLR4 / MD2 is a regulatory molecule in innate immunity. It has a wide range of biological functions in pathophysiological processes.

[0046] The present invention studies the molecular dynamics ...

Embodiment 2

[0053] Example 2 The neutralization and digestion effect of hybrid peptide C-L on LPS

[0054] Dissolve and dilute the hybrid peptide C-L and its parent peptide LL-37 and Cecropin A into solutions of different concentrations (0-64 μg / mL) using pyrogen-free endotoxin test water, and take 100 μL of the above-mentioned peptide solutions of each concentration and LPS (1EU / mL) mixed. After incubation at 37°C for 30 min, the neutralization rate of peptides Cecropin A, LL-37, and C-L to LPS was detected using a chromogenic substrate Limulus kit. The results are shown in Figure 2. Peptide C-L has a higher neutralization and digestion activity of LPS, and its neutralization activity is higher than that of its parent peptide LL-37 and Cecropin A. When 50% LPS neutralization rate was reached, the concentrations of C-L, L, and C were close to 2, 4, and 8 μg / mL, respectively, indicating that the ability of C-L to neutralize LPS was about 2 times and 4 times that of its parent peptides L a...

Embodiment 3

[0055] Example 3 Effect of Hybrid Peptide C-L on Survival Rate of Mouse Macrophage Cells

[0056] The macrophage RAW264.7 in the logarithmic growth phase was inoculated in a 96-well plate, and the initial cell culture density was 1×10 4 cells / mL, 100 μL per well, at 37°C, 5% CO 2 After culturing overnight under certain conditions, a series of concentrations of C-L, LL-37 and Cecropin A (0-100 μg / mL) solutions were added, and after culturing for 24 hours, the CCK8 method was used to detect the effect of polypeptide C-L on the survival rate of mouse macrophages. influences. The result is as image 3 As shown, the cytotoxicity of hybrid peptide C-L was significantly lower than that of its parent peptide, and the survival rate of macrophages was greater than 80% in the concentration range of 0-70 μg / mL, indicating that C-L had lower cytotoxicity and higher security.

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Abstract

The invention relates to a hybrid peptide having functions of detoxification, anti-inflammatory, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and an application thereof. The hybrid peptide of the present invention is named C-L, which is obtained by bioinformatics technology design, heterozygosis, in-vitro and in-vivo test screening and verification for the antibacterial peptide LL-37 and Cecropin A, and an amino acid sequence thereof is shown as SEQ ID NO. 1. C-L not only neutralizes and digests endotoxin, but also inhibits the body's inflammatory response inan inflammatory state. In addition, C-L can alleviate apoptosis caused by exogenous pathogens and protect intestinal barrier integrity and promote wound healing, the C-L has the advantages of low cytotoxicity and high safety, and can be used as an ideal endotoxin antidote (a disinfectant), an anti-inflammatory agent, anti-apoptotic agent, an intestinal barrier integrity protector or a wound healing promoter, and has a good application potential and value.

Description

technical field [0001] The invention relates to the fields of genetic engineering and biological preparations, in particular to a hybrid peptide capable of detoxification, anti-inflammation, anti-apoptosis, protection of intestinal barrier and promotion of wound healing and its preparation method and application. Background technique [0002] Animals or humans often cause inflammatory reactions (redness, heat, swelling, pain, etc.) in the event of body injury, pathogenic bacteria or virus infection, and invasion of toxic and harmful molecules, which in turn lead to metabolic disorders and even death. At present, for infectious inflammation and non-infectious inflammation, the traditional prevention and treatment strategy is to use antibiotics and hormonal anti-inflammatory drugs (such as hydrocortisone, dexamethasone, etc.), but the long-term or excessive use of these drugs has serious risks of. [0003] Although antibiotics can play an anti-inflammatory role by reducing or...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/17A61P31/00A61P39/02A61P29/00A61P1/00A61P39/00A61P17/02A23L33/18A23K20/147
CPCA23V2002/00A61K38/00A23K20/147A23L33/18A61P1/00A61P17/02A61P29/00A61P31/00A61P39/00A61P39/02C07K14/43586C07K14/4723A23V2200/30A23V2250/55
Inventor 张日俊卫旭彪张璐璐斯大勇黄燕
Owner CHINA AGRI UNIV
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