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Detection of organophosphorus pesticides by ratiometric fluorescent sensor based on manganese dioxide nanosheets

A technology of organophosphorus pesticides and fluorescent sensors, which is applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of single response signal interference, etc., and achieve the effect of low raw material cost, short experiment cycle and simple principle

Active Publication Date: 2021-08-24
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most fluorescence assays focus on turn-off or turn-on for detection, and a single response signal is disturbed by various experimental factors

Method used

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  • Detection of organophosphorus pesticides by ratiometric fluorescent sensor based on manganese dioxide nanosheets
  • Detection of organophosphorus pesticides by ratiometric fluorescent sensor based on manganese dioxide nanosheets
  • Detection of organophosphorus pesticides by ratiometric fluorescent sensor based on manganese dioxide nanosheets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Manganese dioxide nanosheet (MnO 2 NS) synthesis

[0035] 10mL of 3wt% hydrogen peroxide was mixed with 10mL of 1.2M tetramethylammonium hydroxide solution and quickly added 10mL of 0.3M MnCl within 15 seconds 2 solution, the solution immediately turned dark brown, and then the suspension was vigorously stirred at room temperature for 12 h, and the resulting solution was centrifuged in a centrifuge for 20 minutes (2000 rpm), then washed three times with water and methanol, and dried at 60 ° C. Dry in an oven to obtain massive manganese dioxide. Weigh 10mg block manganese dioxide and dissolve it in 10mL ultrapure water and sonicate for 12h to completely disperse the manganese dioxide. The dispersion is centrifuged in a centrifuge for 30 minutes (2000rpm), and the supernatant is reserved for the next experiment.

[0036] image 3 A shows the synthesized MnO 2 TEM images of NS, from image 3 It can be seen in A that it has a two-dimensional sheet structu...

Embodiment 2

[0037] Example 2 Manganese dioxide nanosheets (MnO 2 NS) synthesis

[0038] 10mL of 2wt% hydrogen peroxide was mixed with 10mL of 1.1M tetramethylammonium hydroxide solution and quickly added 10mL of 0.2M MnCl within 30 seconds 2 solution, the solution immediately turned dark brown, and then the suspension was vigorously stirred at room temperature for 12 h, and the resulting solution was centrifuged in a centrifuge for 20 minutes (2000 rpm), then washed three times with water and methanol, and dried at 60 ° C. Dry in an oven to obtain massive manganese dioxide. Weigh 10mg block manganese dioxide and dissolve it in 10mL ultrapure water and sonicate for 12h to completely disperse the manganese dioxide. The dispersion is centrifuged in a centrifuge for 30 minutes (2000rpm), and the supernatant is reserved for the next experiment.

Embodiment 3

[0039] Example 3 Acetylcholinesterase induces MnO 2 Nanosheets (MnO 2 NS) decomposition and fluorescence changes of SC and AR

[0040] 0.5 U / mL AChE (50 μL) and 5 mM ATCh (20 μL) were mixed with 30 μL PBS buffer. After incubating at 37°C for 30 minutes, 30 μL of the MnO prepared in Example 1 was added 2 Nanosheet solution (0.5 mg / mL) and 54 μL PB buffer. The resulting mixture was incubated at room temperature for 30 minutes. Next, add 6 µL of SC (50 µM) and 10 µL of AR (50 µM) to the above mixture and make sure the final volume is 200 µL. Fluorescence spectra of different samples were recorded under excitation at 380 nm and 560 nm, respectively, at room temperature. figure 2 A is the fluorescence spectrum of SC and figure 2 B is the fluorescence spectrum of AR,

[0041] ATCh / MnO2 NS / SC / AR (a), ATCh / AChE / MnO2 NS / SC / AR (c).

[0042] 0.5 U / mL AChE (50 μL) and 5 mM ATCh (20 μL) were mixed with 30 μL PBS buffer. After incubating at 37°C for 30 minutes, 30 μL of the MnO p...

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Abstract

The invention discloses an analysis method for detecting organophosphorus pesticides by a ratiometric fluorescent sensor based on the response of manganese dioxide nanosheets. Add acetylcholine chloride solution and PBS buffer solution to react to obtain a solution, add manganese dioxide nanosheet solution and PB buffer solution to the solution and react at room temperature to obtain a mixture, and finally add Scopoletin and Amplex Red to the above mixture to react in a dark environment, record Solution fluorescence spectrum, the concentration of organophosphorus pesticides can be obtained through the fluorescence intensity. The present invention does not require the detection of expensive precision instruments, simplifies the detection method, and greatly reduces the detection cost of organophosphorus pesticides. The present invention has the advantages of low operating cost, quick and easy detection, good selectivity, and the like.

Description

technical field [0001] The invention relates to the field of biological technology, and specifically relates to an analysis method for detecting organophosphorus pesticides by a ratiometric fluorescent sensor based on the response of manganese dioxide nanosheets. Background technique [0002] As common pesticides, organophosphorus pesticides (OPs) are widely used in agricultural production. OPs play a huge role in increasing crop yield and controlling pests and diseases. However, excessive use of OPs can lead to different levels of residues in food, water and the environment. OPs can inhibit the activity of acetylcholinesterase (AChE), seriously affecting human health. Acetylcholinesterase (AChE) is an acetylcholine hydrolase mainly present in the central nervous system of humans and animals. Its basic function is to catalyze the hydrolysis of the neurotransmitter acetylcholine, leading to the termination of nerve impulse transmission, thereby maintaining the normal physi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 卫伟姚田田卫敏刘松琴
Owner SOUTHEAST UNIV
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