Method for promoting spore production of gummy stem blight pathogenic bacteria

A technology of pathogenic bacteria and blight, which is applied in the field of microorganisms, can solve the problems of few pycnidia, difficulty in completing pathogenicity determination and drug screening tests, and restricting the application of pathogenic bacteria of blight, so as to promote the formation and promote nutrition. Effects on Growth and Reproductive Growth

Active Publication Date: 2019-10-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many mediums for cultivating fungi, such as PDA medium, oat medium, corn flour medium, carrot medium, etc., but these mediums cannot meet the requirements of rapid growth of the pathogenic bacteria of sweet potato blight, which has become The bottleneck in the research of this bacterium makes it difficult to complete the pathogenicity assay and drug screening test of this bacterium
In addition, it is difficult to induce the conidia of the fungus Blight blight. Although some specific media can meet the requirements of rapid growth of mycelia, there are few conidia produced, which seriously restricts the pathogenic bacteria Blight blight. Application in related research of sweet potato breeding and pathology

Method used

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  • Method for promoting spore production of gummy stem blight pathogenic bacteria
  • Method for promoting spore production of gummy stem blight pathogenic bacteria
  • Method for promoting spore production of gummy stem blight pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the influence of the culture medium of different carbon source, nitrogen source on the growth of sweet potato blight pathogenic bacteria

[0033] In this example, on the basis of Chapei's medium, the effects of different carbon and nitrogen sources on the pathogen of sweet potato blight were studied by changing the carbon and nitrogen sources.

[0034] The components of Cha's medium are: 1 g of dipotassium hydrogen phosphate, 0.5 g of potassium chloride, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 O) 0.5g, ferrous sulfate is 0.01g, potassium nitrate 3g, sucrose 30g, agar is 20g.

[0035] Maltose, xylose, fructose, lactose, mannose, soluble starch, dextrin, sorbitol, glucose, galactose, trehalose, ribose, cellulose, and rhamnose were used to replace sucrose in Chapei's medium, keeping The nitrogen source in each culture medium was potassium nitrate, and 14 kinds of culture media with different carbon sources were prepared.

[0036] L-cysteine, glycine,...

Embodiment 2

[0055] Embodiment 2: the influence of different mediums on the growth of sweet potato blight pathogenic bacteria

[0056] (1) The preparation of Cha's medium with maltose as the sugar source and peptone as the nitrogen source and the cultivation of the pathogenic bacteria of the blight

[0057] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (0) 0.5g, ferrous sulfate is 0.01g, peptone 3g, maltose 30g, agar are 20g;

[0058] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL, and adjust to pH=7;

[0059] Step 3: Place the prepared medium in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0060] Step 4: Pour the sterilized culture medium into the plate in a sterile environment for standby;

[0061] Step 5: In a sterile environment, use sterilized tweezers to pick up the sweet potato blight pathogenic bacteria cake with...

Embodiment 3

[0093] Embodiment 3: the impact of culture conditions on the spore production of sweet potato blight pathogenic bacteria

[0094] According to the experimental results of Example 2, it is found that dark culture can not induce sporulation of the pathogenic bacterium of the blight, although the growth rate of the pathogen can be improved through the optimization of the medium, it cannot effectively promote the sporulation. Therefore, the present invention further improves the sporulation of the blight The culture conditions of the pathogenic bacteria were optimized in order to obtain a method that can promote the spore production of the pathogenic bacteria of Blight blight.

[0095] In this example, several different culture media were taken as examples to study the mycelium growth and spore production under different culture conditions.

[0096] A: The culture medium with optimized carbon source and nitrogen source, the medium components are: dipotassium hydrogen phosphate 1...

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Abstract

The invention discloses a method for promoting spore production of gummy stem blight pathogenic bacteria. The method comprises the steps of inoculating culture mediums with gummy stem blight pathogenic bacteria, and performing illumination-dark alternate culturing in a constant-temperature incubator so as to obtain spores, wherein the illumination-dark alternate culturing is 12h illumination-12h dark alternate culturing, an illumination light source is an ultraviolet lamp, and the wave length of the ultraviolet lamp is 280nm. The illumination-dark culture time, the illuminant and the wave length of the illuminant are optimized, under the premise that the nutrient growth of mycelia of the gummy stem blight pathogenic bacteria is not influenced, the mycelia are transited from the nutrient growth phase to a generative growth phase, the formation of conidiospores of the gummy stem blight pathogenic bacteria is promoted, massive spores can be obtained within 7 days, and needs for quantity of the conidiospores of the gummy stem blight pathogenic bacteria in actual research work of pathology and breeding science can be sufficiently met.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a culture medium for cultivating the pathogenic bacteria of variegated blight and a cultivation method for promoting the spore production of the pathogenic bacterium of variegated blight. Background technique [0002] Sweet potato (Dioscorea esculenta (Lour.) Burkill) named sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc., is a twining herbaceous vine of Convolvulaceae Dioscorea. Native to tropical America, it was introduced into China at the end of the 16th century, and there are currently more than 500 species. Sweet potatoes are distributed from south to north and from east to west in China, and are widely planted in Sichuan, Henan, Shandong, Guangzhou, Anhui, Zhejiang and other places. my country's sweet potato planting area accounts for about 70% of the world's total. More than 100 million tons, accounting for 80% of the world's total output. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12N3/00C12R1/645
CPCC12N1/14C12N3/00C12N13/00
Inventor 谢昀烨王汉荣方丽王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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