Kit for respiratory pathogen multiple nucleic acid detection

A kit and pathogen technology, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of long cycle, short detection cycle, cumbersome operation, etc.

Active Publication Date: 2019-10-15
DAAN GENE CO LTD
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is complex, cumbersome to operate, long cycle, low sensitivity, relatively high requirements for samples, and prone to false negative results
[0006] Serological testing is based on the principle of antigen-antibody reaction. The detection cycle is short, but the specificity and sensitivity are low, and false negative and false positive results are prone to occur, which has certain limitations.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for respiratory pathogen multiple nucleic acid detection
  • Kit for respiratory pathogen multiple nucleic acid detection
  • Kit for respiratory pathogen multiple nucleic acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] 1. Materials and methods

[0127] 1.1 Simulation samples

[0128] Virus fluid: Influenza A virus, Influenza B virus, Respiratory syncytial virus; Plasmids: Adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Legionella pneumophila, Haemophilus influenzae, plasmids provided by Shanghai Sangon Bioengineering Co., Ltd., and the virus liquid was prepared by Sun Yat-Sen University Daan Gene Co., Ltd.

[0129] 1.2 Clinical samples

[0130] 50 positive samples for respiratory pathogens and 20 negative samples.

[0131] 1.3 Primers and probes

[0132] Through the sequence comparison analysis of the Genebank database and the nucleic acid sequences related to respiratory pathogens reported in published literature at home and abroad, the highly conserved segment with no secondary structure was selected as the amplified target segment, and multiple pairs of primers and probes were artificially designed. Both primers and probes were synthesized by ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a kit for respiratory pathogen multiple nucleic acid detection. In specific, a multiple respiratory pathogen PCR amplification system is designed and verified with experiments to obtain a multiple fluorescence PCR amplification system for detecting a plurality of respiratory pathogens, and 9 respiratory pathogens can be detected simultaneously. The kit has high sensitivity and specificity, and can achieve rapid detection and analysis on multiple respiratory pathogens in nasopharyngeal swabs, alveolar fluid, sputum and other samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a multiple nucleic acid detection kit for respiratory pathogens. Background technique [0002] Acute respiratory tract infection (ARTI) is one of the most common infectious diseases in humans worldwide, and its incidence rate ranks first among acute infectious diseases. On average, lower respiratory tract infection causes about 4000000 patients to die every year. Globally, respiratory infections are the leading cause of death in children under 5 years of age. ARTI is generally caused by bacteria and viruses, about 90% of which are caused by respiratory viruses, but death and severe cases are mainly caused by bacterial infections. In the face of the current complex situation of prevention and control of respiratory infectious diseases, strengthening the monitoring of epidemic information of infectious epidemic strains can provide a basis for formulating prevent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/689C12N15/11
CPCC12Q1/701C12Q1/705C12Q1/689C12Q2600/16
Inventor 蒋析文黄桃生李欣钰郑若楠
Owner DAAN GENE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap