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Method for detecting ascorbic acid

An ascorbic acid and lysine modification technology, which is applied in the field of electrochemistry, can solve the problems of complicated operation steps, expensive instruments, unfavorable rapid analysis, etc., and achieves the effects of good selectivity, low cost and convenient detection device.

Inactive Publication Date: 2019-10-25
BENGBU COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation steps of the above determination methods are cumbersome, time-consuming, expensive to use, and high in analysis costs, so it is not conducive to better rapid analysis

Method used

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  • Method for detecting ascorbic acid
  • Method for detecting ascorbic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of silver-doped poly-L-lysine modified electrode

[0017] A glassy carbon electrode was used as the working electrode, a saturated calomel electrode was used as the reference electrode, and a platinum wire was used as the counter electrode in a three-electrode system containing 1.00×10 -3 mol / L of L-lysine, 3.00×10 -4 In the solution of mol / L silver nitrate, 0.05mol / L nitric acid and 0.10mol / L potassium nitrate, in the potential range of -0.4~2.6V, let it stand still for 5s, and use cyclic voltammetry to scan at a scanning speed of 60mV / s 10 cycles to obtain a silver-doped poly-L-lysine modified electrode.

[0018] The principle of the silver-doped poly-L-lysine modified electrode prepared under the above-mentioned optimal conditions for detecting ascorbic acid is as follows:

[0019] figure 1 The cyclic voltammetry curve (1 to 10 means scanning 1 to 10 circles) of the silver-doped poly-L-lysine modified electrode was prepared under the optimal ...

Embodiment 2

[0021] Embodiment 2 directly measures 8.00×10 -4 mol / L ascorbic acid

[0022] The silver-doped poly-L-lysine modified electrode prepared in Example 1 was put into a phosphate buffer solution of pH=3.0 as a supporting electrolyte, and the phosphate buffer solution was composed of Na 3 PO 4 , NaH 2 PO 4 、Na 2 HPO 4 Composition, the concentration of each substance is 0.1moL / L, using H 3 PO 4 Adjust the pH to 3.0, and after standing still for 14s, in the potential range of -0.6 ~ 0.8V, use cyclic voltammetry at a scanning speed of 160mV / s to detect 8.00×10 -4 mol / L ascorbic acid solution for scanning determination, and then measure a series of ascorbic acid solutions containing known concentrations, and draw a standard curve.

[0023] According to the standard curve, the concentration of ascorbic acid obtained by using the oxidation peak current was 8.112×10 -4 mol / L, with a relative standard deviation of 1.4% from the true value.

Embodiment 3

[0024] Embodiment 3 standard addition method measures the ascorbic acid of unknown concentration

[0025] According to the conditions of Example 2, the modified electrode prepared in Example 1 was placed in a solution containing unknown concentration of ascorbic acid for measurement, and 2.00×10 -3 After mol / L ascorbic acid, measure again.

[0026] The test results showed that the concentration of ascorbic acid in the unknown sample was 4.15×10 -4 mol / L, the recovery rate measured by the standard addition method was 98.21%.

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Abstract

The invention discloses a method for detecting ascorbic acid. The method comprises a step of placing a three-electrode system with a glassy carbon electrode as a working electrode, a saturated calomelelectrode as a reference electrode and a platinum wire as a counter electrode into a solution containing 1.00*10(-3) mol / L of L-Lysine, 3.00*10(-4) mol / L of silver nitrate, 0.05 mol / L of nitric acidand 0.10 mol / L of potassium nitrate, standing for 5 seconds in a potential range of -0.4 to 2.6 V, scanning at a scanning speed of 60 mV / s for 10 cycles by a cyclic voltammetry method to obtain a silver-doped poly-L-lysine modified electrode, a step of using the above modified electrode and measuring an ascorbic acid solution with the phosphate buffer solution with pH of 3.0 as a support electrolyte by using the cyclic voltammetry method in a potential range of -0.6 to 0.8V at the scanning speed of 160 mV / s with the standing time of 14 seconds. A result shows that the oxidation peak current ofthe ascorbic acid shows a good linear relationship with the concentration in the range of 1.00*10(-4) to 5.00*10(-3)mol / L, and the limit of detection is 3.00*10(-5)mol / L. The method can be used for the sensitive detection of the ascorbic acid, and the method has the characteristics of high accuracy, good selectivity, simplicity and easy operation, low cost and fast measurement speed.

Description

technical field [0001] The invention belongs to the technical field of electrochemistry, and in particular relates to a method for detecting ascorbic acid. Background technique [0002] Ascorbic acid, also known as vitamin C (Vitamin C), is a colorless crystal with a sour taste and is easily soluble in water. It is an essential nutrient for higher primates and other few organisms. It plays a very important role in the organism. It can be used as an antioxidant to eliminate the threat of free radicals to the body; The formation of collagen in the body participates in the metabolism of substances in the body. However, vitamin C, as an indispensable nutrient for the human body, cannot be synthesized automatically in the body and can only be taken from the outside. It exists widely in food, animal and plant body fluids and tissues, especially in fresh vegetables and fruits such as kiwi, orange, tomato and pepper. When the human body takes in too much vitamin C, the chances of...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 胡文娜刘伟
Owner BENGBU COLLEGE