Use of IFI44L gene and PI3 gene in preparation of diagnostic bacterial/viral infection kit

Abstract

The invention relates to use of an IFI44L gene and a PI3 gene in preparation of a diagnostic bacterial / viral infection kit. According to the use of the IFI44L gene and the PI3 gene in preparation of the diagnostic bacterial / viral infection kit, it is proved that characteristic change of the IFI44L gene and the PI3 gene exists in diseases infected through bacteria and virus for the first time, thekit for diagnosing bacterial / viral infection is provided, the kit comprises a TaqMan polymerase chain reaction system, a primer pair for amplifying the IFI44L gene and the PI3 gene and reference genes, and a TaqMan fluorescence probe, and the TaqMan polymerase chain reaction system comprises qPCR buffering liquid, dNTPs, TaqDNA polymerase. Compared with the prior art, the accuracy reaches above 90% by using the diagnostic kit for detecting bacterial infection and viral infection, the diagnostic kit has the characteristics of fastness, reliability, high specificity and high sensibility, and theearly diagnosis of infectious diseases is facilitated.

Description

technical field [0001] The invention relates to the use of IFI44L gene and PI3 gene in preparing a kit for diagnosing bacterial / virus infection, and belongs to the technical field of biomedicine. Background technique [0002] Bacterial infection is one of the main diseases that threaten human health. There are traces of bacterial infection on ancient Egyptian mummies. From the "Black Death" that ravaged Europe for many years in the Middle Ages to the multiple outbreaks of cholera in my country in the last century, they are all painful memories of bacterial infections that seriously threaten human health. According to 2017 data, more than 15 million people died directly from bacterial infection in the world. [0003] Since the British Fleming discovered penicillin in 1929, mankind has entered the era of antibiotics, and antibiotics have saved thousands of lives. However, today, more than half a century later, anti-infection treatment has not become easier, on the contrary ,...

AI Technical Summary

Problems solved by technology

According to an authoritative medical review in 2018, the diagnostic accuracy of blood routine is 57.4%, that of CRP is 64.3%, and that of PCT is 65.6%. The specificity and sensitivity of these indicators cannot meet the clinical needs, resulting in misdiagnosis and missed diagnosis of many patients.

The medical community has been trying to find better diagnostic indicators for bacterial infections, but detection methods at the protein level or cell level cannot meet clinical needs

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