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Walnut branch blight antagonizing bacteria and application thereof

A technology of walnut branches and Bacillus subtilis, applied in the fields of microbiology and plant disease prevention and control, can solve the problems of antagonizing Juglanconis juglandina biocontrol strains, etc., and achieve good biocontrol potential and significant effect

Active Publication Date: 2019-11-01
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Walnut branch blight (Juglanconis juglandina) is a newly discovered disease on walnut branches, fruits and leaves in Xinjiang in recent years, and there is no related research on biocontrol strains that can antagonize Juglanconis juglandina

Method used

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  • Walnut branch blight antagonizing bacteria and application thereof
  • Walnut branch blight antagonizing bacteria and application thereof
  • Walnut branch blight antagonizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and identification of Bacillus subtilis (Bacillus subtilis) QB002

[0031] 1. Isolation of antagonistic bacteria

[0032] Disease specimens were collected in different regions of Xinjiang, and bacterial strains were isolated by tissue separation method. Single colony plates with different shapes were picked and cultured and streaked on NA medium. Observation and record of edge shape, etc., pick a single colony and store it at 4°C for later use.

[0033] 2. Identification of antagonistic bacteria

[0034] 2.1 Bacterial morphology observation and Biolog analysis and identification

[0035] Observe the culture morphology and Gram staining of QB002 strain. After the strain QB002 was cultured on NA medium for 24 hours, the single colony was light yellow, irregular in shape, with folds in the middle, opaque, moist in texture and not smooth on the surface. Gram staining is purple, it is Gram-positive bacteria, and the bacteria are short rod-shaped ( f...

Embodiment 2

[0048] The growth inhibitory effect of embodiment 2 bacillus subtilis QB002 to walnut branch blight

[0049] The inhibitory effect of strain QB002 on walnut branch blight was determined by plate confrontation culture method. Take the purified cultured pathogenic bacteria cake with a diameter of 5 mm and place it in the center of the PDA plate. The strain QB002 was activated on NA medium, cultured at constant temperature for 24 hours, a single colony was picked and drawn at a distance of 2 cm from the pathogenic bacteria, and only the pathogenic bacteria were inoculated as a control, and each treatment was repeated 3 times. Cultivate at a constant temperature of 28°C. When the control colonies cover the plate, observe and measure the width of the inhibition zone and the growth diameter of the bacteria, calculate the growth inhibition rate of the colonies, and screen out the bacteria with the best antagonistic effect.

[0050]

[0051] A strain QB002 ( Figure 4 ), its inhi...

Embodiment 3

[0052] Example 3 Preliminary Study on the Antibacterial Mechanism of Bacillus subtilis QB002

[0053] The inhibitory effect of the active substance of the antagonistic strain QB002 on the hyphae of Juglanconis juglandina was carried out using the plate confrontation method. A 5mm diameter bacterial cake of pathogenic bacteria was placed in the center of the PDA plate, and a sterile cover glass was placed around 1cm away from the center of the plate. After the growth of hyphae, the bacteria QB002 was streaked at a distance of 1 cm from the cover glass, and cultured at 30°C for 3 days. Taking the hyphae only inoculated with the pathogen as a control, the effect of the active product of the antagonistic bacterium QB002 on the morphology of the mycelium of the pathogen was observed under an optical microscope. The results showed that the inhibitory rate of antagonistic bacteria on the growth of walnut branch blight pathogen reached 88.6%. The hyphae of the pathogen (Juglanconis j...

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Abstract

The invention relates to a walnut branch blight antagonizing bacteria and application thereof. The bacterium is bacillus subtilis QB002 with a preservation number of CGMCC NO.18218. According to the walnut branch blight antagonizing bacteria and the application thereof, the QB002 can inhibit growth of mycelia of walnut branch blight, causes the mycelia to be deformed and distorted and produce multiple branches, a growth inhibition rate, measured by plate confrontation culture, of QB002 on walnut branch blight pathogen is 88.6%, and moreover, QB002 has the certain inhibitory effect on apple canker, date fruit black spot and Lee brown rot and broad spectrum antimicrobial activity. The bacillus subtilis QB002 has good biological control potential for walnut branch blight, and can be used to control walnut branch blight.

Description

technical field [0001] The invention belongs to the fields of microbiology and plant disease prevention and control, and in particular relates to a bacillus subtilis with good inhibitory effects on various plant pathogens and an application thereof. Background technique [0002] Walnut (Juglans regia L.), also known as walnut, is a woody oil tree species of Juglans genus Juglans. It is the first of the four major dried fruits, and its nutritional value and medicinal value are very high. At present, there are large-scale cultivations in more than 20 provinces and cities in my country, and the planting quantity and output rank first in the world. Xinjiang is one of the origins and main production areas of walnuts. Xinjiang's walnut production has accounted for 15% of the country's total output. The walnut industry has become an important part of Xinjiang's economic development. However, the walnut branch blight caused by the fungus Juglanconis juglandina has occurred seriousl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
CPCC12N1/20C12N1/205C12R2001/125
Inventor 马荣赵颖王敏蔡桂芳王雪萍
Owner XINJIANG AGRI UNIV
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