A Method for Improving Egg Production Performance of Goose
An egg-laying performance and protein technology, which is applied in the field of goose egg-laying, can solve problems such as time-consuming and labor-intensive, and achieve the effects of increasing the number of small follicles and egg production, improving egg-laying performance, and having good application prospects.
Active Publication Date: 2021-08-31
YANGZHOU UNIV
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Problems solved by technology
Huo Mingdong and Chen Yonghua, etc. regulate the light time and light cycle to increase the egg production of geese (how to increase the egg production of mother geese, Huo Mingdong, etc., Waterfowl World, 2011, (1): 18-19; through the regulation of light cycle to improve the egg production of geese Egg-laying performance, Chen Yonghua et al., Waterfowl World, 2011, (2): 41-44); these methods can only improve the egg-laying performance of geese to a certain extent, and are time-consuming and labor-intensive
[0003] Therefore, at present, technical measures such as feed optimization and environmental control are generally used to improve the egg production performance of geese, but these methods can only improve the egg production performance of geese to a certain extent, and are time-consuming and laborious.
Method used
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Embodiment
[0028] (1) Ovary tissues of Zhejiang white geese at the nesting stage were obtained. Total ovarian RNA was extracted by TRIzol method, and cDNA was synthesized.
[0029] (2) Use PCR to amplify the CDS region of the INH-α gene, and the primer information is as follows:
[0030]
[0031] PCR results such as figure 1 As shown, the PCR product was purified, inserted into the NdeI / HindIII restriction site of the PET-30a(+) vector, and double-digested for identification. The result of the double-digestion is as follows figure 2 As shown, the restriction site is NdeI / HindIII.
[0032] (3) Transform the constructed recombinant fragment into a recombinant product to construct a recombinant expression plasmid.
[0033] (4) Transform the recombinant expression plasmid into Escherichia coli E.coli BL21 (purchased from Novagen) and grow to OD 600 When = 0.6-0.8, IPTG with a final concentration of 0.2mM was added to the culture medium to induce at 15°C for 16 hours, and the bacteria...
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The invention discloses a method for improving egg-laying performance of goose, comprising the following steps: (1) extracting total RNA from goose ovary tissue and amplifying the sequence of the CDS region of goose INH-α gene, and inserting it into the prokaryotic expression vector PET-30a( +) In the NdeI / HindIII restriction site of the vector; (2) Transform the above-mentioned vector into the recombinant product to construct a recombinant expression plasmid; (3) Transform the above-mentioned recombinant expression plasmid into E. After crushing, centrifuge to obtain inclusion body containing protein; (4) separate and purify protein from above inclusion body to obtain recombinant fusion protein; (5) make vaccine from purified recombinant fusion protein to immunize laying goose. Compared with the prior art, the method of the present invention can significantly increase the number of small follicles and egg production in laying goose, improve the egg production performance of goose, and is simple and easy to implement, providing a new method for solving the technical bottleneck of low egg production of goose , provides effective technical support for the development of goose industry, and has a good application prospect to immunize laying goose.
Description
technical field [0001] The invention relates to a method for improving goose egg-laying performance, and belongs to the technical field of goose egg-laying. Background technique [0002] China is a big goose breeding and consumption country, and its breeding volume accounts for 93.2% of the world's total, occupying an absolute position. However, the low egg production performance of goose is the key bottleneck restricting the development of goose industry. The heritability of egg production traits of geese is low, and it is difficult to improve the yield performance of geese through conventional breeding. Some scientists are also trying to improve the egg production performance of geese through technological means such as feed optimization and environmental control. For example, Zhao Xin and Long Yingzi improved the egg production of geese by changing the levels of vitamins, daidzein and energy protein and feeding methods (the effect of vitamin, daidzein and energy protein...
Claims
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IPC IPC(8): C12N15/12C12N15/70C07K14/465C07K1/22A61K39/00A61P37/04A01K67/02
CPCA01K67/02A61K39/0005A61K2039/552A61P37/04C07K14/465C12N15/70
Inventor 安晨徐琪陈国宏曹正锋张扬张钰
Owner YANGZHOU UNIV